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HiCT: High Throughput Protocols For CPE Cloning And Transformation
The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High Throughput Circular Extension Cloning and Transformation (HiCT).
Description of construct libraries generated by the HiCT method can be found at http://2013.igem.org/Team:Heidelberg/Indigoidine.
This RFC also points out further optimization strategies with regard to construct stability, reduction of transformation background and the generation of competent cells