Effects of synbiotic supplement on human gut microbiota, body composition and weight loss in obesity

Targeting gut microbiota with synbiotics (probiotic supplements containing prebiotic components) is emerging as a promising intervention in the comprehensive nutritional approach to reducing obesity. Weight loss resulting from low-carbohydrate high-protein diets can be significant but has also been linked to potentially negative health effects due to increased bacterial fermentation of undigested protein within the colon and subsequent changes in gut microbiota composition. Correcting obesity-induced disruption of gut microbiota with synbiotics can be more effective than supplementation with probiotics alone because prebiotic components of synbiotics support the growth and survival of positive bacteria therein. The purpose of this placebo-controlled intervention clinical trial was to evaluate the effects of a synbiotic supplement on the composition, richness and diversity of gut microbiota and associations of microbial species with body composition parameters and biomarkers of obesity in human subjects participating in a weight loss program. The probiotic component of the synbiotic used in the study contained Lactobacillus acidophilus, Bifidobacterium lactis, Bifidobacterium longum, and Bifidobacterium bifidum and the prebiotic component was a galactooligosaccharide mixture. The results showed no statistically significant differences in body composition (body mass, BMI, body fat mass, body fat percentage, body lean mass, and bone mineral content) between the placebo and synbiotic groups at the end of the clinical trial (3-month intervention, 20 human subjects participating in weight loss intervention based on a low-carbohydrate, high-protein, reduced energy diet). Synbiotic supplementation increased the abundance of gut bacteria associated with positive health effects, especially Bifidobacterium and Lactobacillus, and it also appeared to increase the gut microbiota richness. A decreasing trend in the gut microbiota diversity in the placebo and synbiotic groups was observed at the end of trial, which may imply the effect of the high-protein low-carbohydrate diet used in the weight loss program. Regression analysis performed to correlate abundance of species following supplementation with body composition parameters and biomarkers of obesity found an association between a decrease over time in blood glucose and an increase in Lactobacillus abundance, particularly in the synbiotic group. However, the decrease over time in body mass, BMI, waist circumstance, and body fat mass was associated with a decrease in Bifidobacterium abundance. The results obtained support the conclusion that synbiotic supplement used in this clinical trial modulates human gut microbiota by increasing abundance of potentially beneficial microbial species

"Per la molt magnífica senyora e de mi cara jermana la senyora Catarina Çabastida en lo castell de la Brucola, en Sicília". Lettere di donne catalane del quattrocento

The work illustrates some aspects of woman’s Middle Ages with help of unpublished letters found in the Archives of "Centre Borja" of S. Cugat del Vallés (Barcelona). The documents wrote by two catalan women show the life and the mentality of themselves who lived between Sicily and Barcelona at the end of fifteenth century

Assessing the Sensitivity of the Non-Hydrostatic Regional Climate Model to Boundary Conditions and Convective Schemes over the Philippines

Regional climate models have been useful in climate studies and in downscaling climate projections from global climate models, especially for areas characterized by complex topography and coastline features, such as the Philippines. However, several factors may affect model skill, such as uncertainties related to the boundary conditions and model configuration. This study evaluates the performance of the non-hydrostatic regional climate model (NHRCM) over the Philippines. Present-day climate simulations at 50 km resolution are conducted using two sets of boundary conditions (ECMWF ERA-Interim and the NCEP/NCAR Reanalysis Project NNRP1), as well as two convective parameterization schemes in the model (Grell and Kain-Fritsch). Results show that the seasonal changes in the spatial distribution of temperature, rainfall, and winds over the Philippines are simulated reasonably well. NHRCM has an overall cold and dry bias over land, the degree of which depends on the boundary condition and the convective scheme used. After adjusting the simulated temperature because of the difference in topography, the temperature differs from that observed by -0.90°C to -0.42°C on average. The rainfall bias in NHRCM ranges from -62.13 % to -25.20 %. Regardless of the boundary condition, the Grell scheme results in the lowest temperature bias with high skill scores, while the Kain-Fritsch scheme gives the lowest rainfall bias with high correlation and skill scores. The boundary conditions also influence model skill, such that the model bias is lower for temperature when ERA-Interim is used, but lower for rainfall with NNRP1. NHRCM represents the seasonal cycles of temperature and rainfall for all regions, but tends to generate more occurrences of cold and dry months. Improvements in the model are still possible, but these results indicate the potential of the model to be used for providing essential information for describing historical and future changes in the Philippine climate

Effects of glaucoma and snoring on cerebral oxygenation in the visual cortex: a study using functional Near Infrared Spectroscopy (fNIRS)

Purpose: The purpose of this study was to investigate the effects of snoring and glaucoma on the visual Haemodynamic Response (HDR) using functional Near Infrared Spectroscopy (fNIRS). Methods: We recruited 8 glaucoma patients (aged 56-79), 6 habitual snorers (aged 26-61) and 10 healthy control participants (aged 21-78). Glaucoma patients were of varying subtypes and under care of ophthalmologists. Prior to testing visual acuity, blood pressure, heart rate and a medical history were taken. HDRs were recorded over the primary visual cortex (V1) using a reversing checkerboard paradigm. Results & Discussion: All participants showed the characteristic increase of Oxyhaemoglobin concentration ([HbO]) and decrease of Deoxyhaemoglobin concentration ([HbR]) during visual stimulation (p < 0.001, η2 = 0.78). Despite this, there were signifi cant group differences with a large effect size (η2 = 0.28). During visual stimulation normal participants had greater [HbO] compared to snorers and glaucoma patients (p < 0.01). Both glaucoma patients and snorers presented with comparable HDR for [HbO] and [HbR] in V1. Importantly, during visual stimulation, the increased [HbO] in glaucoma patients correlated well with their visual fi elds and self-reported activities of daily living (r = -0.98, r = -0.82, p < 0.05). Both glaucoma patients and snorers presented with an attenuated HDR in V1. Our results suggest a possible vascular link between these conditions

Increased risk of depression in non-depressed HIV infected men with sleep disturbance: Prospective findings from the Multicenter AIDS Cohort Study.

ObjectiveSleep disturbance is a known risk factor for depression, but it is not known whether sleep disturbance contributes to greater risk of depression in those infected with human immunodeficiency virus (HIV+) as compared to those uninfected with HIV (HIV-).MethodsUsing data from the Multicenter AIDS Cohort Study, a population-based prospective study of men who have sex with men (MSM), self-reported sleep disturbance (>2 weeks) and depressive symptoms (Clinical Epidemiologic Scale for Depression, CES-D) were assessed every 6 months over 12 years of follow-up. Adjusted mixed effects logistic regression analyses tested whether sleep disturbance predicted depression (CES-D ≥ 16) at the immediate subsequent visit, and so on over 12 years, in non-depressed HIV+(N = 1054; 9556 person-visits) and non-depressed HIV- (N = 1217; 12,680 person-visits). In HIV+ vs. HIV- MSM, linearly estimated average incidence of depression and normalized cumulative rate of depression over 12 years were compared.ResultsIn the HIV+ MSM, sleep disturbance was associated with a significant increase in depression 6 months later (OR = 1.6; 95% CI, 1.30, 1.96), which was significantly greater (P < .05) than in HIV- MSM (OR = 1.16; 95% CI, 0.94, 1.44). HIV status and sleep disturbance interacted (P < .001), such that incidence of depression and normalized cumulative rate of depression were greater in HIV+ with sleep disturbance than in HIV+ without sleep disturbance and HIV- groups (all P's < 0.001).ConclusionsHIV+ persons who report sleep disturbance represent a high risk group to be monitored for depression, and possibly targeted for insomnia treatment to prevent depression. FUND: National Institute of Allergy and Infectious Diseases

Practices of place-making through locative media artworks

Peer-reviewedIn recent years, the vast increase in information flows has made it possible to instantly connect location-dependent information with physical spaces. These technologies have provided new forms of the representation of space as much as new forms of perception through tools and techniques used in land surveying, remote sensing, etc. From a critical point of view, pervasive computing, location-based applications, or, in other words, "locative media" provide an interesting framework to understand how these technologies relate to our understanding of space and place. Concretely, we want to examine how the uses of locative media in social-oriented artworks interact with people's sense of place. This article therefore discusses contemporary theories on space related to media and technology with a specific focus on the conceptualization of the notion of place. It also relates these theories to the study of different locative media artworks: Canal Accessible (2006), Bio Mapping (2004), Disappearing Places (2007), and Coffee Deposits (2010). We contend that locative media artworks act upon distinctive ways to understand the mediation of technology in current placemaking practices.En los últimos años, el aumento de los flujos de información ha hecho posible conectar instantáneamente la información dependiente de ubicación con los espacios físicos. Estas tecnologías han facilitado tanto nuevas formas de representación del espacio como nuevas formas de percepción a través de herramientas y técnicas usadas en topografía, teledetección, etc. Desde un punto de vista crítico, la computación ubicua, las aplicaciones de localización, o en otras palabras, los "medios locativos" proporcionan un marco interesante para entender cómo estas tecnologías se relacionan con nuestra comprensión del espacio y el lugar. Concretamente, queremos examinar cómo los usos de los medios locativos en la vida social orientados a obras de arte interactuan con el sentimiento de lugar. En este artículo se analizan tanto las teorías contemporáneas sobre el espacio en relación con los medios de comunicación y la tecnología con un enfoque específico en la conceptualización de la noción de lugar. Estas teorías se refieren también al estudio de diferentes obras de arte de medios locativos: Canal Accesible (2006), Bio Mapping (2004), Lugares Disappearing (2007), y los restos de café (2010). Sostenemos que las obras de arte de medios locativos actuan de maneras distintas para entender la mediación de la tecnología en las prácticas de Placemaking actuales.En els últims anys, l'augment dels fluxos d'informació ha fet possible connectar instantàniament la informació dependent d'ubicació amb els espais físics. Aquestes tecnologies han facilitat tant noves formes de representació de l'espai com noves formes de percepció a través d'eines i tècniques utilitzades en topografia, teledetecció, etc. Des d'un punt de vista crític, la computació ubiqua, les aplicacions de localització o, en altres paraules, els "mitjans locatius" proporcionen un marc interessant per entendre com aquestes tecnologies es relacionen amb la nostra comprensió de l'espai i el lloc. Concretament, volem examinar com els usos dels mitjans locatius en la vida social orientats a obres d'art interactuen amb el sentiment de lloc. En aquest article s'analitzen tant les teories contemporànies sobre l'espai en relació amb els mitjans de comunicació i la tecnologia amb un enfocament específic en la conceptualització de la noció de lloc. Aquestes teories es refereixen també a l'estudi de diferents obres d'art de mitjans locatius: Canal Accessible (2006), Bio Mapping (2004), Llocs Disappearing (2007), i les restes de cafè (2010). Sostenim que les obres d'art de mitjans locatius actuen de maneres diferents per entendre la mediació de la tecnologia en les pràctiques de Placemaking actuals

Approaches to Avoid Proteolysis During Protein Expression and Purification

All cells contain proteases, which hydrolyze the peptide bonds between amino acids of a protein backbone. Typically, proteases are prevented from nonspecific proteolysis by regulation and by their physical separation into different subcellular compartments; however, this segregation is not retained during cell lysis, which is the initial step in any protein isolation procedure. Prevention of proteolysis during protein purification often takes the form of a two-pronged approach: first, inhibition of proteolysis in situ, followed by the early separation of the protease from the protein of interest via chromatographic purification. Protease inhibitors are routinely used to limit the effect of the proteases before they are physically separated from the protein of interest via column chromatography. In this chapter, commonly used approaches to reducing or avoiding proteolysis during protein expression and purification are reviewed

Protein Extraction and Purification by Differential Solubilization

The preparation of purified soluble proteins for biochemical studies is essential and the solubility of a protein of interest in various media is central to this process. Selectively altering the solubility of a protein is a rapid and economical step in protein purification and is based on exploiting the inherent physicochemical properties of a polypeptide. Precipitation of proteins, released from cells upon lysis, is often used to concentrate a protein of interest before further purification steps (e.g., ion exchange chromatography, size exclusion chromatography etc). Recombinant proteins may be expressed in host cells as insoluble inclusion bodies due to various influences during overexpression. Such inclusion bodies can often be solubilized to be reconstituted as functional, correctly folded proteins. In this chapter, we examine strategies for extraction/precipitation/solubilization of proteins for protein purification. We also present bioinformatic tools to aid in understanding a protein’s propensity to aggregate/solubilize that will be a useful starting point for the development of protein extraction, precipitation, and selective re-solubilization procedures