Immunophenotyping of the XY donor-derived cells in the endometria of transplanted patients.

<p>(A) XY donor-derived cells (indicated by arrows, orange and green signals) in the absence of the hematopoietic marker CD45+ (blue signal) in the recipients' endometria. Lower panel: spleen as a positive control for CD45 (blue). (B) Co-localization of XY donor-derived cells (indicated by arrows) with epithelial marker CD9 (blue) in recipients' endometria indicate the coexistence of XY+CD9+ cells in epithelial glands. Lower panel: epithelial endometrium as a positive control for CD9 (blue). (C) Co-localization of XY donor-derived cells (indicated by arrows) with stromal marker Vm (blue) in recipients' endometria, indicated by the overlapping of the XY+Vm+ expression in the stromal fraction. Lower panel: stromal endometrium as a positive control for Vm (blue). All the images were acquired using a 63×/1.7× oil immersion objective with a confocal microscope. Note that DAPI signals are shown in gray in all the images.</p

Characteristics of transplant recipients.

<p>CBSC, Cord Blood Stem Cell; BM, Bone Marrow; PBSC, Peripheral Blood Stem Cells; HRT, Hormone Replacement Therapy. (*atrophic endometrium).</p

Isolation of endometrial SP cells and FISH analyses from BMT recipients.

<p>(A) Table indicating the percentages of freshly endometrial SP cells obtained in all 5 patients by a cell sorter analysis. Percentages are provided for both the epithelial and stromal fractions. (B) Diagram showing a typical SP graph and the results of the FISH analyses of epithelial and stromal SP cells, revealing the absence of XY donor-derived cells.</p

Analysis of the telomere length regions in the endometria of BMT recipients.

<p>Assessment of telomere length in the endometrial sections from BMT recipients: note that patients 1 and 2 are represented. A color code on the left-hand side of the image symbolizes the degree of colors corresponding to telomeric length, where long and short telomeres are represented in red and blue, respectively. Also note how the regions associated with long telomeres are located mainly in the stromal compartment.</p

Detection and quantification of XY cells from donor origin in the endometria of BMT recipients.

<p>(A&B) Human endometrial sections from 2 different patients, who underwent BMT, showing a positive FISH signal corresponding to chromosome Y (green signal) and chromosome X (orange signal) along both the epithelial and stromal compartments. Presence of donor-derived cells (chromosome Y-positive cells) in the endometrium is indicated by arrows. Lower panels containing control slides: (A) endometrium as a positive control for the chromosome X signal and (B) fetal testes as a positive control for the chromosome XY signal. (C) Table summarizing the characteristics of the recipients, the percentages of XY-positive cells obtained in the whole endometrium, plus the epithelial and stromal fractions. All the images were acquired using a 63×/1.6× oil immersion objective.</p