Biomimetic Electrospun Self-Assembling Peptide Scaffolds for Neural Stem Cell Transplantation in Neural Tissue Engineering

Spinal cord regeneration using stem cell transplantation is a promising strategy for regenerative therapy. Stem cells transplanted onto scaffolds that can mimic natural extracellular matrix (ECM) have the potential to significantly improve outcomes. In this study, we strived to develop a cell carrier by culturing neural stem cells (NSCs) onto electrospun 2D and 3D constructs made up of specific crosslinked functionalized self-assembling peptides (SAPs) featuring enhanced biomimetic and biomechanical properties. Morphology, architecture, and secondary structures of electrospun scaffolds in the solid-state and electrospinning solution were studied step by step. Morphological studies showed the benefit of mixed peptides and surfactants as additives to form thinner, uniform, and defect-free fibers. It has been observed that β-sheet conformation as evidence of self-assembling has been predominant throughout the process except for the electrospinning solution. In vitro NSCs seeded on electrospun SAP scaffolds in 2D and 3D conditions displayed desirable proliferation, viability, and differentiation in comparison to the gold standard. In vivo biocompatibility assay confirmed the permissibility of implanted fibrous channels by foreign body reaction. The results of this study demonstrated that fibrous 2D/3D electrospun SAP scaffolds, when shaped as micro-channels, can be suitable to support NSC transplantation for regeneration following spinal cord injury

New records of the rufous-tailed hawk (Buteo ventralis) in Argentine Patagonia

Presentamos nuevos datos acerca de la distribución del Aguilucho Cola Rojiza (Buteo ventralis) en el oeste de la Patagonia argentina. Se describe un avistaje en la provincia de Río Negro. Se señalan nuevas localidades en las provincias de Neuquén, Río Negro y Chubut, en las que la especie ha sido observada. La especie está asociada a ambientes boscosos, especialmente bosques puros o mixtos de Nothofagus spp.We present new data on the distribution of the Rufous-tailed Hawk (Buteo ventralis) in western Argentine Patagonia. We describe a sighting in Río Negro province. We report new sites in Neuquén, Río Negro and Chubut provinces where this hawk has been observed. The species is associated to forests, especially pure or mixed Nothofagus spp. forests

Novel peptidomimetics related to Gonadotropin releasing hormone (GnRH)

Novel GnRH I and II analogues were designed and synthesized by Solid Phase Peptides Synthesis (SPPS), since GnRH has antiproliferative property, but poor metabolic stability. To rationalize synthetic difficulties, molecular dynamics simulations were performed, showing the conformational behavior of three derivatives. Among the two peptidomimetics series (Ie,f and IIe,f , GnRH I and GnRH II analogues respectively) several compounds (Id-f and IIc-e) showed a significant binding affinity. In particular, derivative Ie has an increased metabolic stability with respect to the physiological ligand (Ie t1/2= 3.96 h versus GnRH I t1/2 = 2.63 h)

Nuevos datos acerca del estatus del Aguilucho Andino Buteo albigula en la Patagonia Argentina

New data on the status of Buteo albigula in Argentina Patagonia. We list new records of B. albigula in Nahuel Huapi National Park, and neighboring regions (Argentine Patagonia). Data show that the species is more common thas previously thought, perhaps because it is poorly know and hard to observe. B albigula is found in forested areas and its survival is cleary tied to that one of the forest in Argentine Patagonia and elsewhere

Towards the Inhibition of Protein–Protein Interactions (PPIs) in STAT3: Insights into a New Class of Benzothiadiazole Derivatives

Signal transducer and activator of transcription 3 (STAT3) is a validated anticancer target due to the relationship between its constitutive activation and malignant tumors. Through a virtual screening approach on the STAT3-SH2 domain, 5,6-dimethyl-1H,3H-2,1,3-benzothiadiazole-2,2-dioxide (1) was identified as a potential STAT3 inhibitor. Some benzothiadiazole derivatives were synthesized by employing a versatile methodology, and they were tested by an AlphaScreen-based assay. Among them, benzosulfamide 1 showed a significant activity with an IC50 = 15.8 ± 0.6 µM as a direct STAT3 inhibitor. Notably, we discovered that compound 1 was also able to interact with cysteine residues located around the SH2 domain. By applying mass spectrometry, liquid chromatography, NMR, and UV spectroscopy, an in-depth investigation was carried out, shedding light on its intriguing and unexpected mechanism of interaction

Preliminary investigation on feline coronavirus presence in the reproductive tract of the tom cat as a potential route of viral transmission

Objectives: Feline infectious peritonitis (FIP) is an immune-mediated disease initiated by feline coronavirus (FCoV) infection. To date, the only proven route of transmission is the faecal\u2013oral route, but a possible localisation of FCoV in the reproductive tract of tom cats is of concern, owing to the involvement of the male reproductive tract during FIP and to the presence of reproduction disorders in FCoV-endemic feline catteries. The aim of the study was to investigate the presence and localisation of FCoV in semen and/or in the reproductive tract of tom cats, and its possible association with seroconversion and viraemic phase. Methods: Blood, serum, semen and/or testicle samples were obtained from 46 tom cats. Serology was performed on 38 serum samples, nested reverse transcriptase PCR (nRT-PCR) and reverse transcriptase quantitative PCR (RT-qPCR) were performed on 39 blood samples and on 17 semen samples, and histology, immunohistochemistry and nRT-PCR were performed on 39 testicles. Results: Twenty-four of 38 serum samples were positive on serology. Semen samples were negative on RT-PCR and RT-qPCR for FCoV, while all blood samples were negative at both molecular methods, except for one sample positive at RT-qPCR with a very low viral load. All testicles were negative at immunohistochemistry, while six were positive at nRT-PCR for FCoV. Serology and blood PCR results suggest that the virus was present in the environment, stimulating transient seroconversion. FCoV seems not to localise in the semen of tom cats, making the venereal route as a way of transmission unlikely. Although viral RNA was found in some testicles, it could not be correlated with the viraemic phase. Conclusions and relevance: In the light of these preliminary results, artificial insemination appears safer than natural mating as it eliminates the direct contact between animals, thus diminishing the probability of faecal\u2013oral FCoV transmission

6-Hydroxy-2-methylbenzofuran-4-carboxylic acid

6-Hydroxy-2-methylbenzofuran-4-carboxylic acid was synthesized in two steps, starting from 3,5-dihydroxybenzoate. The product was obtained through a direct thermal one-pot cyclization with propargyl bromide, followed by a base-catalyzed hydrolysis. Its molecular structure was elucidated by means of mono- and bidimensional NMR techniques, ESI-MS, FT-IR and single-crystal X-ray diffraction

Towards the Inhibition of Protein–Protein Interactions (PPIs) in STAT3: Insights into a New Class of Benzothiadiazole Derivatives

Signal transducer and activator of transcription 3 (STAT3) is a validated anticancer target due to the relationship between its constitutive activation and malignant tumors. Through a virtual screening approach on the STAT3-SH2 domain, 5,6-dimethyl-1H,3H-2,1,3-benzothiadiazole-2,2-dioxide (1) was identified as a potential STAT3 inhibitor. Some benzothiadiazole derivatives were synthesized by employing a versatile methodology, and they were tested by an AlphaScreen-based assay. Among them, benzosulfamide 1 showed a significant activity with an IC50 = 15.8 \ub1 0.6 \ub5M as a direct STAT3 inhibitor. Notably, we discovered that compound 1 was also able to interact with cysteine residues located around the SH2 domain. By applying mass spectrometry, liquid chromatography, NMR, and UV spectroscopy, an in-depth investigation was carried out, shedding light on its intriguing and unexpected mechanism of interaction

Novel self-assembling cyclic peptides with reversible supramolecular nanostructures

Self-assembly peptides (SAPs) are an important class of hydrogels used in nanomedicine for tissue repair and neural regeneration. Due to their unique properties, SAPs may be used in a wide range of applications but some limitations, such as low bioavailability and rapid hydrolysis degradation, need to be overcome. Here, we describe the synthesis and characterization of two novel cyclic SAPs without the use of d/l-alternating amino acids, showing a reversible transition of their supramolecular nanostructures, from nanotubes/nanofibers into nanovesicles/nanospheres. The investigation, characterization and optimization are performed using atomic force microscopy (AFM), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, Raman analysis, circular dichroism (CD), and rheology measurements. Also, in vitro cell viability assays show negligible toxicity of the representative optimized cyclic SAP towards human neural stem cells (hNSCs). Our results suggest that linear SAP theoretical background can be applied to develop cyclic SAPs, with important implications in the scalable fabrication of inter-changeable nanostructures, as well as for biomedical applications, including tissue regeneration, drug-delivery, drug-design, sensing, imaging, and size selectivity

Experimental aerothermal characterization of surface air-cooled oil coolers for turbofan engines

[EN] Thermal management is a major challenge for new generation turbofan aero-engines. One of the most promising heat exchangers are the so-called surface air-cooled oil coolers (SACOCs). In this study, an experimental methodology is proposed and implemented in order to characterize SACOCs mounted in turbofan bypass ducts. Three different SACOC geometries have been characterized under the same nominal operating point, while the actual velocity profile in the bypass was reproduced by means of a distortion screen upstream the test section. The heat exchangers were mounted in counterflow configuration and feature the same fin geometry in the oil side. The three prototypes varied only in the air side, being the first a baseline flat plate, the second a SACOC with standard trapezoidal fins and the third featuring optimized fins designed to reduce the pressure drop. Aerothermal results demonstrated that the effect of the SACOC on the bypass flow was confined to a region about the same height and width of the finned area, avoiding the need of reproducing the whole bypass duct. However, for this reduced-height experimental approach to be valid, we show that the velocity profile needs to be rearranged to match the specific section of the whole bypass. We also demonstrate how the optimized fin geometry achieved a 10% lower friction factor than the standard one at nominal flow conditions while increasing the overall heat transfer coefficient by 5.2%.This project has received funding from the Clean Sky 2 Joint Undertaking under the European Union's Horizon 2020 research and innovation programme under grant agreement n° 831977: Aerodynamic upgrade of Surface Air-Cooled Oil Coolers (SACOC). A. Felgueroso is supported through the Programa de Apoyo para la Investigación y Desarrollo of the Universitat Politècnica de València under grant PAID-01-20 n° 21589. The authors also wish to thank Safran Aircraft Engines for their kind permission to share the data presented in this publication. Special thanks are also given to Mr. Adolfo Guzmán for his inestimable support during the experimental campaign.Broatch, A.; Olmeda, P.; Garcia Tiscar, J.; Felgueroso-Rodríguez, A.; Chávez-Modena, M.; González, LM.; Gelain, M.... (2022). Experimental aerothermal characterization of surface air-cooled oil coolers for turbofan engines. International Journal of Heat and Mass Transfer. 190:1-13. https://doi.org/10.1016/j.ijheatmasstransfer.2022.12277511319