FMR Study of Co/Ti Bilayer Thin Films

. We focused on the interaction between two ferromagnetic cobalt layers through a non-magnetic titanium layer. The magnetic properties of the structure were characterized by ferromagnetic resonance technique (FMR). The data were collected as a function of non-magnetic titanium layer thickness. Co/Ti multilayer (Ti (50 {\AA})/Co(45 {\AA})/Ti(2-40 {\AA})/Co(40 {\AA})/Ti(100 {\AA}))films were grown onto naturally oxidized p-type single crystal Si (100) substrate at UHV condition with magnetron sputtering system at room temperature. The thickness of Ti spacer layer ranges from 2 to 40 {\AA} with 2 {\AA} steps. We did not observe usual optic and acoustic modes; instead we had two broad overlapped peaks for the films ranged from 6 {\AA} to 40 {\AA}. One interesting result was the high anisotropic resonance field values for these films. Exchange coupling between ferromagnetic layers causes shift on resonance field values but these shifts in our samples were much larger than expected. This large anisotropic behavior is not clear at the moment. Our theoretical model was not able to determine a value for the exchange coupling parameter. One reason can be the close thickness values for Co sublayers. The other reason can be the Ti non-magnetic layer. If titanium did not grow layer by layer on cobalt, the cobalt ferromagnetic layers may behave as a single layer. As a result one cannot observe exchange interaction between ferromagnetic layers through non-magnetic spacer.Comment: 6 pages, 5 figure

Assessing the in vivo biocompatibility of molecularly imprinted polymer nanoparticles

Molecularly imprinted polymer nanoparticles (nanoMIPs) are high affinity synthetic receptors which show promise as imaging and therapeutic agents. Comprehensive analysis of the in vivo behaviour of nanoMIPs must be performed before they can be considered for clinical applications. This work reports the solid-phase synthesis of nanoMIPs and an investigation of their biodistribution, clearance and cytotoxicity in a rat model following both intravenous and oral administration. These nanoMIPs were found in each harvested tissue type, including brain tissue, implying their ability to cross the blood brain barrier. The nanoMIPs were cleared from the body via both faeces and urine. Furthermore, we describe an immunogenicity study in mice, demonstrating that nanoMIPs specific for a cell surface protein showed moderate adjuvant properties, whilst those imprinted for a scrambled peptide showed no such behaviour. Given their ability to access all tissue types and their relatively low cytotoxicity, these results pave the way for in vivo applications of nanoMIPs

Inactivation of the Complement Lectin Pathway by Candida tropicalis Secreted Aspartyl Protease-1

Copyright © 2022 The Authors. Candida tropicalis is an opportunistic fungal pathogen and is one of the most frequently isolated non-albicans species. It can cause localised as well as invasive systemic infections particularly in immunocompromised patients. Increased resistance to common anti-fungal drugs is an emerging problem. In order to establish disseminated infections, Candida has evolved several strategies to escape the host immune system. A detailed understanding of how C. tropicalis escapes the host immune attack is needed as it can help develop novel anti-fungal therapies. Secreted aspartyl proteinases (Saps) of C. albicans have been shown to be determinants of virulence and immune evasion. However, the immune evasion properties of C. tropicalis Saps have been poorly characterised. This study investigated the immune evasion properties of C. tropicalis secreted aspartic protease 1 (Sapt1). Sapt1 was recombinantly produced using a Kluyveromyces lactis yeast expression system. A range of complement proteins and immunogloublins were screened to test if Sapt1 had any proteolytic activity. Sapt1 efficiently cleaved human mannose-binding lectin (MBL) and collectin-11, which are the initiating molecules of the lectin pathway of the complement system, but not L-ficolin. In addition, Sapt1 cleaved DC-SIGN, the receptor on antigen presenting dendritic cells. Proteolysis was prominent in acidic condition (pH 5.2), a characteristic of aspartyl protease. No proteolytic activity was detected against complement proteins C1q, C3, C3b, IgG and IgA. In view of the ability of Sapt1 to cleave MBL and collectin-11, we found that Sapt1 could prevent activation of the complement lectin pathway. RT-qPCR analysis using three different C. tropicalis clinical isolates (oral, blood and peritoneal dialysis fluid) revealed relatively higher levels of mRNA expression of Sapt1 gene when compared to a reference strain; Sapt1 protein was found to be secreted by all the tested strains. Lectin pathway and its initiating components are crucial to provide front line defence against Candida infections. For the first time, we have shown that a Candida protease can proteolytically degrade the key initiating components of lectin pathway and inhibit complement activation. Findings from this study highlight the importance of exploring Sapt1 as a potential therapeutic target. We conclude that C. tropicalis secretes Sapt1 to target the complement lectin pathway, a key pattern recognition and clearance mechanism, for its survival and pathogenesis

Erratum to: 36th International Symposium on Intensive Care and Emergency Medicine: Brussels, Belgium. 15-18 March 2016.

[This corrects the article DOI: 10.1186/s13054-016-1208-6.]

Erratum to: 36th International Symposium on Intensive Care and Emergency Medicine

[This corrects the article DOI: 10.1186/s13054-016-1208-6.]

Sediment and water influxes into the Black Sea by Anatolian rivers

Average annual sediment load and water inputs of the Anatolian rivers are estimated at 24 million t/y (ca 20 % of the total sediment carried into the Black Sea) and 40 km(3)/y. This load was 53 million t/y before 1988, when two dam constructions were completed near the mouths of the Yesilirmak and Kizilirmak Rivers. Most of the sediment is carried by rivers of the central and eastern sections of the Black Sea coast. The North Anatolian (Pontic) Mountain ranges parallel to the coast are the major sources of sediment. Sediment yields and water discharges reflect altitude and amount of precipitation, decreasing from east to west in the Black Sea coastal Region. A major part of the fluvial sediment yield from the central and eastern regions is first deposited in the continental shelf and then rapidly transported to the deep basin via submarine canyons. Seasonal variation of the fluvial sediment inputs matches well with the cyclicity of the black lamination in the last 3,000 years

COMPLICATED HYDATID CYSTS OF THE LIVER

In 328 cases of hydatid disease of the liver operated between 1979 and 1989, 51 (15.8 %) had complicated cysts. The most frequent complication was intrabiliary rupture (2/3 of all complications). The others were intraperitoneal rupture in 7 cases (13.8 %), suppuration in 7 cases (13.8 %) and intrathoracic rupture in 3 cases (5.8 %). Among the intrabiliary ruptured cysts, T-drainage was performed in 15 cases, choledochoduodenostomy was performed in 12 cases, and sphincteroplasty was performed in 7 cases. The cases of intraabdominal rupture were operated immediately. The suppurated cysts were marsupialized in 3 cases and drained in 4 cases. The 3 cases of bronchobiliary fistula were treated by T-tube drainage of the common bile duct, drainage of the pleural space, repair of the diaphragm and closure of the communicating bronchus. In this series, the mortality rate was 7.8 % and the reccurrence rate was 9.8 %

The effect of prolactin and bromocriptine on human peripheral immune status

The aim of this study was to determine the influence of elevated serum prolactin (PRL) levels on the peripheral lymphocyte subsets in patients with hyperprolartinemia. For this purpose we studied 20 hyperprolactinemic patient lymphocyte subsets by flow cytometry on their hyperprolactinemic state and after their serum prolactin concentration was normalized with bromocriptine (BC) alone or BC and surgery. We observed decreased absolute numbers and percentage of Natural Killer (p=0.0009 and 0.0001, respectively) and CD3/CD25 lymphocytes (p= 0.009 and 0.002) in hyperprolactinemic patients, compared to 8 sex- and age-matched normal controls. There was no correlation between PRL levels and CD16/56 and CD3/CD25 numbers (p=0.72 and 0.33, respectively). We did not find any significant difference in absolute numbers (p=0.95) and percentage (p=0.84) of B-lymphocytes of hyperprolactinemic patients, as compared with normal controls. We did not detect any increase in absolute cell numbers of CD16/CD56 (p=0.21) and CD3/CD25 (p=0.61) of BC-treated patients when compared to their hyperprolactinemic state. We demonstrated an increase in CD8-cells (p=0.0173) and a decrease in CD4/CD8 ratio (p=0.036) in hyperprolactinemic patients treated with BC. There was also an increase in the number of activated T-cells (CD3/HLA DR) in this group, compared to normal controls and the hyperprolactinemic state of the same patients (p=0.04). In conclusion, elevated PRL levels do not lead to an "overstimulation" of the B-cells, but deteriorate the cytotoxic function

Giant Cell Tumor of the Frontal Bone in an 18-Month-Old Girl: A Case Report

Introduction: Giant cell tumors (GCT) are benign, but locally aggressive primary bone neoplasms, that frequently occur in the epiphyses of the long bones. Less than 1% of all GCTs primarily involve the skull where they are preferentially seen in the sphenoid and temporal bones. In the pediatric age group they are exceptionally rare