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    Effect of multiwalled carbon nanotubes on the growth of bacteria of the genus Bacillus and on the production of biosurfactants

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    Celem bada艅 by艂a ocena wzrostu oraz zdolno艣ci do produkcji biosurfaktant贸w trzech szczep贸w bakterii z rodzaju Bacillus hodowanych w obecno艣ci wielo艣ciennych nanorurek w臋glowych. W wyniku bada艅 stwierdzono, 偶e bakterie te bardzo dobrze ros艂y w obecno艣ci nanorurek w臋glowych oraz produkowa艂y biosurfaktanty. Wytworzone przez bakterie biosurfaktanty powodowa艂y dyspersj臋 nanorurek w臋glowych w hodowlach p艂ynnych. Wielo艣cienne nanorurki w臋glowe nie wp艂ywa艂y na morfologi臋 i wzrost bakterii, stymulowa艂y jednak tworzenie przetrwalnik贸w. Badania obejmowa艂y r贸wnie偶 ocen臋 w艂a艣ciwo艣ci produkowanych biosurfaktant贸w przez bakterie rosn膮ce w obecno艣ci nanorurek w臋glowych, tj. napi臋cia powierzchniowego, zdolno艣ci emulguj膮cych i pieni膮cych. Nie stwierdzono wp艂ywu badanych nanorurek w臋glowych na w艂a艣ciwo艣ci powierzchniowe biosurfaktant贸w produkowanych przez te bakterie. Biosurfaktanty zmniejsza艂y napi臋cie powierzchniowe, mia艂y zdolno艣ci emulguj膮ce w stosunku do wybranych substancji/mieszanin hydrofobowych oraz zdolno艣ci pieni膮ce por贸wnywalne z pr贸bk膮 kontroln膮.Three bacterial strains of the genus Bacillus were tested in order to ascertain how the presence of nanotubes in the culture medium influenced their growth, as well as their ability to produce biosurfactants. It was found that the bacteria grew very well in the presence of the nanotubes, and that they were capable of producing biosurfactants. The biosurfactants synthesized by the bacteria accounted for the dispersion of the carbon nanotubes in liquid cultures. Although the multiwalled carbon nanotubes did not affect the morphology or the growth of the bacteria, they were found to stimulate endospore production. The objective of the study was also to assess the properties of the biosurfactants produced by the bacteria growing in the presence of the carbon nanotubes, i.e. surface tension, emulsification and foamability. The nanotubes examined were found to exert no influence on the surface activity of the biosurfactants produced by the bacteria. The biosurfactants reduced surface tension, showed emulsifying properties towards some hydrophobic substances/mixtures, and displayed foamabilities comparable to those of the control
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