Life history traits of <i>P</i>. <i>pacificus</i> cultured on the yeasts <i>C</i>. <i>albidus</i> and C. <i>curvatus</i>.

<p>The boxplots show the median, first and third quartile values from different life history traits experiments. A) Brood size of <i>P</i>. <i>pacificus</i> grown on <i>C</i>. <i>albidus</i> (C3), <i>C</i>. <i>curvatus</i> (C5) and <i>E</i>. <i>coli</i> OP50. Brood size was calculated as average number of progeny of 10 J4 larvae. B) Pharyngeal pumping rate results of <i>P</i>. <i>pacificus</i> exposed to <i>Cryptococcus</i> species. Assay was performed using <i>E</i>. <i>coli</i> OP50 as control. C) Defecation time (10 mins) of <i>P</i>. <i>pacificus</i> exposed to <i>C</i>. <i>albidus</i> (C3) and <i>C</i>. <i>curvatus</i> (C5) using <i>E</i>. <i>coli</i> OP50 as control. D) Eurystomatous (Eu) ratio of <i>P</i>. <i>pacificus</i> adults grown on <i>C</i>. <i>albidus</i> (C3) and <i>C</i>. <i>curvatus</i> (C5). Eu ratio was calculated from five biological replicates. Statistical significance was calculated using student t-test.</p

Developmental timing and survival curves of <i>P</i>. <i>pacificus</i> after exposure to <i>C</i>. <i>albidus</i> and <i>C</i>. <i>curvatus</i>.

<p>A) The boxplots show the time needed for <i>P</i>. <i>pacificus</i> nematodes to enter different developmental stage when growing on <i>E</i>. <i>coli</i> OP50 or <i>Cryptococcus</i> strains. The inlay shows the individual data points representing ten plates per food source. B) Survival of <i>P</i>. <i>pacificus</i> exposed to <i>C</i>. <i>albidus</i> (C3) and <i>C</i>. <i>curvatus</i> (C5) for 10 days. Standard lab food <i>E</i>. <i>coli</i> OP50 was used as a control.</p

Differential Interference Contrast microscopy (DIC) to study nature of yeast clumps.

<p>A) Yeast cells clumps of <i>C</i>. <i>albidus (C3)</i> in intestine of <i>P</i>. <i>pacificus</i>. B), C) Absence of yeast clumps in defecated worms after 3 hours off food, suggesting that yeasts cells can be fully digested or excreted by <i>P</i>. <i>pacificus</i>.</p

Gene expressions profiling of <i>P</i>. <i>pacificus</i> after a <i>Cryptoccocus</i> or <i>E</i>. <i>coli</i> diet.

<p>A) PCA of transcriptomes obtained from <i>P</i>. <i>pacificus</i> adult worms growing on <i>C</i>. <i>albidus</i> (C3), <i>C</i>. <i>curvatus</i> (C5) and <i>E</i>. <i>coli</i> OP50. Despite substantial variation in the control samples grown on <i>E</i>. <i>coli</i> OP50, different samples cluster together according to their food source indicating robust expression changes under different environments. B) Genes that are down-regulated upon <i>Cryptococcus</i> diet show a bias towards higher expression at early developmental stages. The graph shows mean expression and standard error of down-regulated genes in 10 developmental transcriptomes representing dauer, J2, J3, J4, and adult worms. C) Genes that are up regulated upon <i>Cryptococcus</i> tend to reflect transcriptomes from later developmental stages. D) Enrichment of significantly differentially expressed genes upon exposure to <i>C</i>. <i>albidus (C3)</i> in previous gene expression profiling studies. E) Enrichment of significantly differentially expressed genes upon exposure to <i>C</i>. <i>curvatus (C5)</i> in previous gene expression profiling studies. F) KEGG pathway enrichment of down-regulated genes under exposure to <i>C</i>. <i>albidus (C3)</i>. The x-axis shows the enrichment score indicating how much more genes of a given pathway are found as differentially expressed when compared to random gene sets. The y-axis shows the negative logarithm of the p-value indicating the significance of the overrepresentation. G) KEGG pathway enrichment of up-regulated genes under exposure to <i>C</i>. <i>albidus</i> (C3). H) Enrichment of KEGG pathways among down-regulated genes when fed with <i>C</i>. <i>curvatus</i> (C5). I) Enrichment of KEGG pathways among up-regulated genes after exposure to <i>C</i>. <i>curvatus</i> (C5).</p