The Measurement of Activity of the Ca2+_Mg2+ ATPase in Membranous Vesicles Isolated from Smooth Muscle of Ileum in Rats Treated with Ultraviolet Radiation

The Ca2+_Mg2+ ATPase are high attraction calcium pump, that contributes in maintaining plasma membrane of cytoplasm Ca2+, Mg2+ homeostasis by source to the outside of cell. The aim of the study is to evaluate the effect of the ultraviolet radiation on the activity of the Ca2+_Mg2+ ATPase in the membranous vesicles of ileum in rats. Thirty adult Sprague–Dawley rats (age, 3-4 months, weight range, 180 – 200 g) were used in this experiment, which divided in to 5 groups (n = 6 / group). The membrane vesicles isolated from smooth muscles of rats showed high activity Ca2+_Mg2+ ATPase. All isolated membranous vesicles are irradiated with Ultraviolet radiation of 250 nanometers except control group. The irradiation period for each group was (5, 10, 30 and 45) minutes, respectively. The activity of Ca2+_Mg2+ ATPase was decreased with increased time of irradiation. In conclusion, the increased time of irradiation inhibited Ca2+_Mg2+ ATPase activity isolated from ileum smooth muscles of rats. The recommendations are to expose other organs like liver and kidney to UV radiation to explain its effect or using other range of UV radiation to reflect its effect

Detection Some Trace Elements in Human Milk and Effect of Some Factors on its Concentrations

Study aim to determination some trace elements in lactating mothers human milk and study effect different factors on its concentration, Age , Body mass index, Home and Smoking habitat, atomic absorption flame less used to measured (Mn, Cd, Co and Zn) in 70 sample of lactating mothers milk which digested using tricolor acetic acid 24%. Result show that concentration of these elements increasing than normal value of these elements,Mn was 1801.40, Cd was 114.64, Co was 288.52 and Zn was 212.10.Also factors were studied effect on minerals concentration,  increasing age was  non effected on minerals concentration, over weight causes increasing it, urban resident causesincreasing in Mn concentration while rural residence causes increasing in others minerals, negative smoking habitat causes increasing in Cd and Co concentrations. Key words: trace element, lactating mother’s human milk, atomic absorptio

The effect of Cyclophosphamide on spermatogenesis in rats

This study aimed to evaluate the effects of cyclophosphamide on spermatogenesis; we used three doses with variable time interval to determine the effect of the low and high doses of cyclophosphamide. The results showed that low doses for long time interval caused a considerable increase in the percentage of sperm head abnormalities (Tertatospermia), without any significant changes in tissue sections, the percentage of sperm head abnormalities was increased to 20.72% with the dose 5mg/Kg, while high doses caused a significant tissue changes in testes, and epididymis as well as head sperm abnormalities but less than the 5mg/Kg dose. The percentage of head sperm abnormalities were 14.75 and 13.19 for the doses 15mg/Kg and 10mg/Kg respectively