implication for assessing the risk of arbovirus outbreaks

Funding Information: This work was also supported by the U.S. Centers for Disease Control and Prevention through a cooperative agreement number 5NU14GH001237-03-00. The views expressed in this written publication do not necessarily reflect the official policies of the U.S. Department of Health and Human Services. European Foundation Initiative into Neglected Tropical Disease also supported this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank all community leaders, household heads staff from the provincial health directorates, staff from the Medical Entomology Laboratory from the National Institute of Health, staff from National Malaria Control Programme, and staff from the Laboratório de Transmissores de Hematozoários of the Oswaldo Cruz Institute for their support during field work, laboratory testing and identification of Aedes spp. We also thank José Feriano Américo who produced the maps of this work. Ours special thanks goes to Professor J. Dereck Charlwood who revised the English grammar and typos of the entire manuscript. Consent for publication Our manuscript does not present any individual person's data. Publisher Copyright: © 2018 Abílio et al. http://creativecommons.org/licenses/by/4.0/.Background: Aedes-borne arboviruses have emerged as an important public health problem worldwide and, in Mozambique, the number of cases and its geographical spread have been growing. However, information on the occurrence, distribution and ecology of Aedes aegypti and Ae. albopictus mosquitoes remain poorly known in the country. Methods: Between March and April 2016, a cross-sectional study was conducted in 32 districts in Mozambique to determine the distribution and breeding sites of Ae. aegypti and Ae. albopictus. Larvae and pupae were collected from a total of 2,807 water-holding containers using pipette, dipper, funnel and sweeping procedures, depending on the container type and location. Both outdoor and indoor water-holding containers were inspected. The immature forms were reared to adults and the identifications of the mosquito species was carried out with a stereomicroscope using a taxonomic key. Results: Aedes aegypti was found in every district sampled, while Ae. albopictus was only found in Moatize district, situated in Tete Province in the central part of the country. Six hundred and twenty-eight of 2,807 (22.4%) containers were positive for Ae. aegypti but only one (0.03%) was positive for Ae. albopictus. The Container Index (CI) of Aedes was highest in densely populated suburban areas of the central region (260/604; 43.0%), followed by suburban areas in northern areas (228/617; 36.9%) whilst the lowest proportion was found in urbanized southern areas (140/1586; 8.8%). The highest CI of Aedes was found in used tires (448/1268; 35.3%), cement tanks (20/62; 32.3%) and drums (21/95; 22.1%). Conclusion: Data from our study showed that Ae. aegypti is present nation-wide, since it occurred in every sampled district, whilst Ae. albopictus had a limited distribution. Therefore, the risk of transmission of dengue and chikungunya is likely to have been underestimated in Mozambique. This study highlights the need for the establishment of a national entomological surveillance program for Aedes spp. in Mozambique in order to gain a better understanding about vector bionomics and to support the development of informed effective vector control strategies.publishersversionpublishe

Distribution and breeding sites of Aedes aegypti and Aedes albopictus in 32 urban/peri-urban districts of Mozambique: implication for assessing the risk of arbovirus outbreaks

Submitted by Sandra Infurna ([email protected]) on 2019-02-19T13:42:20Z No. of bitstreams: 1 gabrielagarcia_marianafdavid_etal_IOC_2018.pdf: 3042517 bytes, checksum: 816c1d8c25307b7acf7d49089e5ffbad (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2019-02-19T13:53:12Z (GMT) No. of bitstreams: 1 gabrielagarcia_marianafdavid_etal_IOC_2018.pdf: 3042517 bytes, checksum: 816c1d8c25307b7acf7d49089e5ffbad (MD5)Made available in DSpace on 2019-02-19T13:53:12Z (GMT). No. of bitstreams: 1 gabrielagarcia_marianafdavid_etal_IOC_2018.pdf: 3042517 bytes, checksum: 816c1d8c25307b7acf7d49089e5ffbad (MD5) Previous issue date: 2018Ministry of Health. National Institute of Health. Maputo, Mozambique.Ministry of Health. National Institute of Health. Maputo, Mozambique.Ministry of Health. National Institute of Health. Maputo, Mozambique.Ministry of Health. National Directorate of Public Health. Maputo, Mozambique.Ministry of Health. National Institute of Health. Maputo, Mozambique.Ministry of Health. National Institute of Health. Maputo, Mozambique.Ministry of Health. National Institute of Health. Maputo, Mozambique.Ministry of Health. National Institute of Health. Maputo, Mozambique.Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. GHTM. Lisboa, Portugal.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários Rio de Janeiro, RJ. Brasil.Ministry of Health. National Institute of Health. Maputo, Mozambique.Aedes-borne arboviruses have emerged as an important public health problem worldwide and, in Mozambique, the number of cases and its geographical spread have been growing. However, information on the occurrence, distribution and ecology of Aedes aegypti and Ae. albopictus mosquitoes remain poorly known in the country