Dynamic scaffolds for neuronal signaling: in silico analysis of the TANC protein family

AbstractThe emergence of genes implicated across multiple comorbid neurologic disorders allows to identify shared underlying molecular pathways. Recently, investigation of patients with diverse neurologic disorders found TANC1 and TANC2 as possible candidate disease genes. While the TANC proteins have been reported as postsynaptic scaffolds influencing synaptic spines and excitatory synapse strength, their molecular functions remain unknown. Here, we conducted a comprehensive in silico analysis of the TANC protein family to characterize their molecular role and understand possible neurobiological consequences of their disruption. The known Ankyrin and tetratricopeptide repeat (TPR) domains have been modeled. The newly predicted N-terminal ATPase domain may function as a regulated molecular switch for downstream signaling. Several putative conserved protein binding motifs allowed to extend the TANC interaction network. Interestingly, we highlighted connections with different signaling pathways converging to modulate neuronal activity. Beyond a known role for TANC family members in the glutamate receptor pathway, they seem linked to planar cell polarity signaling, Hippo pathway, and cilium assembly. This suggests an important role in neuron projection, extension and differentiation.</jats:p

Suppression of cell-spreading and phagocytic activity on nano-pillared surface: in vitro experiment using hemocytes of the colonial ascidian Botryllus schlosseri.

Nano-scale nipple array on the body surface has been described from various invertebrates including endoparasitic and mesoparasitic copepods, but the functions of the nipple array is not well understood. Using the hydrophilized nanopillar sheets made of polystyrene as a mimetic material of the nipple arrays on the parasites\u2019 body surface, we assayed the cell spreading and phagocytosis of the hemocytes of the colonial ascidian Botryllus schlosseri. On the pillared surface, the number of spreading amebocytes and the number of phagocytizing hemocytes per unit area were always smaller than those on the flat surface (Mann-Whitney test, p < 0.05 - 0.001), probably because the effective area for the cell attachment on the pillared surface is much smaller than the area on the flat sheet. The present results supports the idea that the nipple array on the parasites' body surface reduces the innate immune reaction from the host hemocytes

Enhancement of superconductivity near the ferromagnetic quantum critical point in UCoGe

We report a high-pressure single crystal study of the superconducting ferromagnet UCoGe. Ac-susceptibility and resistivity measurements under pressures up to 2.2 GPa show ferromagnetism is smoothly depressed and vanishes at a critical pressure $p_c = 1.4$ GPa. Near the ferromagnetic critical point superconductivity is enhanced. Upper-critical field measurements under pressure show $B_{c2}(0)$ attains remarkably large values, which provides solid evidence for spin-triplet superconductivity over the whole pressure range. The obtained $p-T$ phase diagram reveals superconductivity is closely connected to a ferromagnetic quantum critical point hidden under the superconducting `dome'.Comment: 4 pages, 3 figures; accepted for publication in PR

Performance of a large limited streamer tube cell in drift mode

The performance of a large (3x3 $cm^2$) streamer tube cell in drift mode is shown. The detector space resolution has been studied using cosmic muons crossing an high precision silicon telescope. The experimental results are compared with a GARFIELD simulation.Comment: 18 pages, 7 figures. Accepted by Nucl. Instr. and Methods

The Spread of MaaS Initiatives in Europe: The Leading Role of Public Governance Emerging from an Italian Regional Application

Mobility as a service (MaaS) is a promising lever to achieve a more sustainable, co-modal, and flexible urban system for daily mobility. MaaS is crucial to addressing the challenges imposed by hyper-urbanization, social changes, saturation of public soil with private motor vehicles, and local and global environmental issues. The aim of this paper is to analyse important characteristics of MaaS, namely public governance, regional scale, and high level of development. A total of 13 MaaS initiatives implemented in some European cities were compared using multi-criteria analysis. The results show that, although the MaaS implementation process is faster for private initiatives, public governance is shown to be the most appropriate entity to develop stable MaaS services, as it can enable the achievement of a higher level of integration, coupled with a high rate of service adoption, as well as reduce market entry barriers for service providers while also guaranteeing fair competition, and consequently fostering a higher level of service provided to users. The regional coverage of MaaS is crucial to ensure service provision to a wider population, living in both metropolitan and rural areas. These important features are included in the BIPforMaaS project run by the Italian region of Piedmont, described in detail in this paper

Muon spin rotation and relaxation in the superconducting ferromagnet UCoGe

We report zero-field muon spin rotation and relaxation measurements on the superconducting ferromagnet UCoGe. Weak itinerant ferromagnetic order is detected by a spontaneous muon spin precession frequency below the Curie temperature $T_C = 3$ K. The $\mu^+$ precession frequency persists below the bulk superconducting transition temperature $T_{sc} = 0.5$ K, where it measures a local magnetic field $B_{loc} = 0.015$ T. The amplitude of the $\mu$SR signal provides unambiguous proof for ferromagnetism present in the whole sample volume. We conclude ferromagnetism coexists with superconductivity on the microscopic scale.Comment: 4 pages, 3 figures, accepted for publication in PR

Dynamic scaffolds for neuronal signaling: in silico analysis of the TANC protein family.

Fiesta de Tosantos (1101001

Surveillance for Antimicrobial Resistance in Croatia

This study intended to verify, through microbiological techniques and TEM investigations, the killing of bacterial spores after treatment in steam autoclave, and to propose strictly morphological considerations about the target of this sterilisation process. Autoclave is the most common device for sterilising instruments in order to prevent cross infections in dental offices. The autoclave efficiency has been improved in the last years and part of this improvement is related to both a better and more correct use of the autoclave system and to the technological innovations introduced in the last generation of devices. However, associations as ADA or CDC suggest to regularly verify the process of 'autoclaving' through biological indicators (BI). The most commonly used BI are made of spores strips or suspensions of Bacillus Subtilis (pb 168) and Bacillus Stearothermophilus (ATCC 10149). They visually prove, changing colours on enzymatic base, the death of micro-organism and if the physical parameters, necessary for sterilisation, have been achieved. These two strains of endospore-forming bacteria were processed and prepared following two different techniques: Karnovsky fixed and epon embedded--phosphotungstic acid fixed for direct observation. The kind and the extent of analysed modifications are extremely various: from deep lacerations, which changed the spore structure, to little clefts which let the cytoplasm go out

NMR quantification of 16-O-methylcafestol and kahweol in Coffea canephora var. robusta beans from different geographical origins

Diterpenes have recently received a great deal of interest as tools to investigate the botanical origin of coffee. Specifically, kahweol has been proposed as a marker of Coffea arabica while 16-O-methylcafestol (16-OMC) is a Coffea canephora specific marker and its detection and quantification allow the authenticity of pure C. arabica roasted coffee blends to be assessed. In this study, we evaluated the possibility of the industrial use of the quantification of these diterpenes to assess the relative amounts of the two coffee species in blends. The content of 16-OMC and kahweol was determined in 78 samples (i.e., 39 green and the corresponding 39 roasted beans) of C. canephora from different geographical origins using a recently published NMR approach. Our results show a small natural variability in 16-OMC content for the Asian samples (average content = 1837 \ub1 113 mg/kg) while a much larger spread was found for the African samples (average content = 1744 \ub1 322 mg/kg). This large variability prevents the use of 16-OMC to quantify C. canephora in unknown roasted coffee blends. We also show that kahweol cannot be considered a specific C. arabica marker since it was detected almost all coffees and quantified in about 30% of the C. canephora samples