Subcomplex Iλ Specifically Controls Integrated Mitochondrial Functions in Caenorhabditis elegans

Complex I dysfunction is a common, heterogeneous cause of human mitochondrial disease having poorly understood pathogenesis. The extensive conservation of complex I composition between humans and Caenorhabditis elegans permits analysis of individual subunit contribution to mitochondrial functions at both the whole animal and mitochondrial levels. We provide the first experimentally-verified compilation of complex I composition in C. elegans, demonstrating 84% conservation with human complex I. Individual subunit contribution to mitochondrial respiratory capacity, holocomplex I assembly, and animal anesthetic behavior was studied in C. elegans by RNA interference-generated knockdown of nuclear genes encoding 28 complex I structural subunits and 2 assembly factors. Not all complex I subunits directly impact respiratory capacity. Subcomplex Iλ subunits along the electron transfer pathway specifically control whole animal anesthetic sensitivity and complex II upregulation, proportionate to their relative impairment of complex I-dependent oxidative capacity. Translational analysis of complex I dysfunction facilitates mechanistic understanding of individual gene contribution to mitochondrial disease. We demonstrate that functional consequences of complex I deficiency vary with the particular subunit that is defective

Interaction of low-intensity laserm light with human blood

The processes of light attenuation in biological media are considered in details. The techniques of defining attenuation coefficient, plotting scattering indicatrix, calculating erythrocytes, measuring blood temperature under the action of laser radiation are described. The coefficient values depending on the radiation wave length and concentration of scattering centre have been obtained in the experiments. The angular radiation dispersion is plotted, the temperature dynamics is found out. Influence of erythrocytes on laser light attenuation when passing through blood is described. The graphs of attenuation coefficient dependence on the quantity of red cells per 1 mkl of blood for the wavelength of 632 and 890 nm are plotted. Plotting indicatrix and temperature measurement is performed for the wavelength of 632 nm