Técnicas de gestión de conocimiento aplicadas a la evaluación de impacto ambiental

AMBIENTALIA: MEDIO RURAL Y SOSTENIBILIDAD IV CONGRESO ANDALUZ DE DESARROLLO SOSTENIBLE VIII CONGRESO ANDALUZ DE CIENCIAS AMBIENTALE

Turismo micológico y desarrollo sostenible del medio rural en Soria

XII Coloquio de Geografía del Turismo, Ocio y Recreación de la Asociación de Geógrafos Españoles. Colmenarejo (Madrid), del 17 al 19 de junio de 2010.Este trabajo se ha realizado dentro del proyecto de investigación CS02008-03877 (Ministerio de Educación y Ciencia y PEDER

Each Mycobacterium Requires a Specific Culture Medium Composition for Triggering an Optimized Immunomodulatory and Antitumoral Effect

Mycobacterium bovis bacillus Calmette-Guérin (BCG) remains the first treatment option for non-muscle-invasive bladder cancer (BC) patients. In research laboratories, M. bovis BCG is mainly grown in commercially available media supplemented with animal-derived agents that favor its growth, while biomass production for patient treatment is performed in Sauton medium which lacks animal-derived components. However, there is not a standardized formulation of Sauton medium, which could affect mycobacterial characteristics. Here, the impact of culture composition on the immunomodulatory and antitumor capacity of M. bovis BCG and Mycolicibacterium brumae, recently described as efficacious for BC treatment, has been addressed. Both mycobacteria grown in Middlebrook and different Sauton formulations, differing in the source of nitrogen and amount of carbon source, were studied. Our results indicate the relevance of culture medium composition on the antitumor effect triggered by mycobacteria, indicating that the most productive culture medium is not necessarily the formulation that provides the most favorable immunomodulatory profile and the highest capacity to inhibit BC cell growth. Strikingly, each mycobacterial species requires a specific culture medium composition to provide the best profile as an immunotherapeutic agent for BC treatment. Our results highlight the relevance of meticulousness in mycobacteria production, providing insight into the application of these bacteria in BC research

Challenge 6: Open Science: reproducibility, transparency and reliability

Open Science is becoming a new paradigm in scientific research and complex changes are being done. This new way in knowledge development requires a great transformation that will allow science to adapt efficiently and effectively to the urgency of the problems to be solved while ensuring the reproducibility, transparency and reliability of scientific results. This chapter analyzes the impact of this change of model, the challenges to be addressed and the expected benefits.Peer reviewe

Sistema de guiado para movimiento autónomo de vehículos en entornos estructurados

Número de publicación: ES2401509 A1 (22.04.2013) También publicado como: ES2401509 B1 (05.03.2014) Número de Solicitud: Consulta de Expedientes OEPM (C.E.O.) P201101119 (05.10.2011)El objetivo de la invención es un sistema de guiado de vehículos autónomos mediante cámaras y/o fotodetectores para seguir una trayectoria, que se determina por un conjunto de emisores láser dispuestos en un entorno estructurado, por ejemplo, en los distintos pasillos de un invernadero, y que determina la trayectoria a seguir. Para el establecimiento de la trayectoria a seguir, se dispone de diversos emisores láser colocados en los pasillos del invernadero, que estarán activos en función de los pasillos que deba recorrer el vehículo para describir la trayectoria prevista.Universidad de Almerí

The AMIGA sample of isolated galaxies XIV. Disc breaks and interactions through ultra-deep optical imaging

Context. In the standard cosmological model of galaxy evolution, mergers and interactions play a fundamental role in shaping galaxies. Galaxies that are currently isolated are thus interesting because they allow us to distinguish between internal and external processes that affect the galactic structure. However, current observational limits may obscure crucial information in the low-mass or low-brightness regime. Aims. We use optical imaging of a subsample of the AMIGA catalogue of isolated galaxies to explore the impact of different factors on the structure of these galaxies. In particular, we study the type of disc break as a function of the degree of isolation and the presence of interaction indicators such as tidal streams or plumes, which are only detectable in the ultra-low surface brightness regime. Methods. We present ultra-deep optical imaging in the r band of a sample of 25 low-redshift (z< 0.035) isolated galaxies. Through careful data processing and analysis techniques, the nominal surface brightness limits achieved are comparable to those to be obtained on the ten-year LSST coadds (μr,lim ≳ 29.5 mag arcsec-2 [3σ; 10'' × 10'']). We place special emphasis on preserving the low surface brightness features throughout the processing. Results. The extreme depth of our imaging allows us to study the interaction signatures of 20 galaxies since Galactic cirrus is a strong limiting factor in the characterisation of interactions for the remaining 5 of them. We detect previously unreported interaction features in 8 (40% ± 14%) galaxies in our sample. We identify 9 galaxies (36% ± 10%) with an exponential disc (Type I), 14 galaxies (56% ± 10%) with a down-bending (Type II) profile, and only 2 galaxies (8% ± 5%) with up-bending (Type III) profiles. Isolated galaxies have considerably more purely exponential discs and fewer up-bending surface brightness profiles than field or cluster galaxies. We find clear minor merger activity in some of the galaxies with single exponential or down-bending profiles, and both of the galaxies with up-bending profiles show signatures of a past interaction. Conclusions. We show the importance of ultra-deep optical imaging in revealing faint external features in galaxies that indicate a probable history of interaction. We confirm that up-bending profiles are likely produced by major mergers, while down-bending profiles are probably formed by a threshold in star formation. Unperturbed galaxies that slowly evolve with a low star formation rate could induce the high rate of Type I discs in isolated galaxies. © The Authors 2023.We thank Ignacio Trujillo for helpful insights about this work and Aaron Watkins for providing us with the implementation of the automatic break detection method. PMSA, JHK, and JR acknowledge financial support from the State Research Agency (AEI-MCINN) of the Spanish Ministry of Science and Innovation under the grant "The structure and evolution of galaxies and their central regions" with reference PID2019-105602GBI00/10.13039/501100011033, from the ACIISI, Consejeria de Economia, Conocimiento y Empleo del Gobierno de Canarias and the European Regional Development Fund (ERDF) under grant with reference PROID2021010044, and from IAC project P/300724, financed by the Ministry of Science and Innovation, through the State Budget and by the Canary Islands Department of Economy, Knowledge and Employment, through the Regional Budget of the Autonomous Community. JR acknowledges funding from University of La Laguna through the Margarita Salas Program from the Spanish Ministry of Universities ref. UNI/551/2021-May 26, and under the EU Next Generation. LVM acknowledges financial support from grants CEX2021-001131-S funded by MCIN/AEI/ 10.13039/501100011033, RTI2018-096228-B-C31 and PID2021-123930OB-C21 by MCIN/AEI/ 10.13039/501100011033, by "ERDF A way of making Europe" and by the "European Union" and from IAA4SKA (R18-RT-3082) funded by the Economic Transformation, Industry, Knowledge and Universities Council of the Regional Government of Andalusia and the European Regional Development Fund from the European Union. SC acknowledges funding from the State Research Agency (AEI-MCINN) of the Spanish Ministry of Science and Innovation under the grant "Thick discs, relics of the infancy of galaxies" with reference PID2020-113213GA-I00. MAF acknowledges support from FONDECYT iniciacion project 11200107 and the Emergia program (EMERGIA20_38888) from Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades and University of Granada. PMSA and LVM acknowledge the Spanish Prototype of an SRC (SPSRC) service and support funded by the Spanish Ministry of Science, Innovation and Universities, by the Regional Government of Andalusia, by the European Regional Development Funds and by the European Union NextGenerationEU/PRTR. The SPSRC acknowledges financial support from the State Agency for Research of the Spanish MCIU through the "Center of Excellence Severo Ochoa" award to the Instituto de Astrofisica de Andalucia (SEV-2017-0709) and from the grant CEX2021-001131-S funded by MCIN/AEI/ 10.13039/501100011033. Based on observations made with the Isaac Newton Telescope operated on the island of La Palma by the Isaac Newton Group of Telescopes in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofisica de Canarias. The WFC imaging was obtained as part of the programs C163/13A, C106/13B, and C106/14A. Based on observations collected at the European Organisation for Astronomical Research in the Southern Hemisphere under ESO programme(s) 098.B-0775(A), 093.B-0894(A). Based on data collected at the Subaru Telescope and retrieved from the HSC data archive system, which is operated by Subaru Telescope and Astronomy Data Center at National Astronomical Observatory of Japan

Mycobacteria emulsified in olive oil-in-water trigger a robust immune response in bladder cancer treatment

The hydrophobic composition of mycobacterial cell walls leads to the formation of clumps when attempting to resuspend mycobacteria in aqueous solutions. Such aggregation may interfere in the mycobacteria-host cells interaction and, consequently, influence their antitumor effect. To improve the immunotherapeutic activity of Mycobacterium brumae, we designed different emulsions and demonstrated their efficacy. The best formulation was initially selected based on homogeneity and stability. Both olive oil (OO)- and mineral oil-in-water emulsions better preserved the mycobacteria viability and provided higher disaggregation rates compared to the others. But, among both emulsions, the OO emulsion increased the mycobacteria capacity to induce cytokines' production in bladder tumor cell cultures. The OO-mycobacteria emulsion properties: less hydrophobic, lower pH, more neutralized zeta potential, and increased affinity to fibronectin than non-emulsified mycobacteria, indicated favorable conditions for reaching the bladder epithelium in vivo. Finally, intravesical OO-M. brumae-treated mice showed a significantly higher systemic immune response, together with a trend toward increased tumor-bearing mouse survival rates compared to the rest of the treated mice. The physicochemical characteristics and the induction of a robust immune response in vitro and in vivo highlight the potential of the OO emulsion as a good delivery vehicle for the mycobacterial treatment of bladder cancer

Celia's encephalopathy and c.974dupG in BSCL2 gene: a hidden change in a known variant

This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s10048-019-00574-5Celia’s encephalopathy (progressive encephalopathy with/without lipodystrophy (PELD)) is a childhood neurodegenerative disorder with a fatal prognosis before the age of 10, due to the variant c.985C>T in the BSCL2 gene that causes a cryptic splicing site leading to skipping of exon 7. For years, different authors have reported cases of congenital generalized lipodystrophy due to the variant c.974dupG in BSCL2 associated with neurological manifestations of variable severity, although some of them clearly superimposable to PELD. To identify the molecular mechanisms responsible for these neurological alterations in two patients with c.974dupG. Clinical characterization, biochemistry, and neuroimaging studies of two girls carrying this variant. In silico analysis, PCR amplification, and BSCL2 cDNA sequencing. BSCL2-201 transcript expression, which lacks exon 7, by qPCR in fibroblasts from the index case, from a healthy child as a control and from two patients with PELD, and in leukocytes from the index case and her parents. One with a severe encephalopathy including a picture of intellectual deficiency, severe language impairment, myoclonic epilepsy, and lipodystrophy as described in PELD, dying at 9 years and 9 months of age. The other 2-year-old patient showed incipient signs of neurological involvement. In silico and cDNA sequencing studies showed that variant c.974dupG gives rise to skipping of exon 7. The expression of BSCL2-201 in fibroblasts was significantly higher in the index case than in the healthy child, although less than in the case with homozygous PELD due to c.985C>T variant. The expression of this transcript was approximately half in the healthy carrier parents of this patient. The c.974dupG variant leads to the skipping of exon 7 of the BSCL2 gene and is responsible for a variant of Celia’s encephalopathy, with variable phenotypic expression.S

SKA Science Data Challenge 2: analysis and results

EDELWEISS Collaboration: P. Hartley et al.The Square Kilometre Array Observatory (SKAO) will explore the radio sky to new depths in order to conduct transformational science. SKAO data products made available to astronomers will be correspondingly large and complex, requiring the application of advanced analysis techniques to extract key science findings. To this end, SKAO is conducting a series of Science Data Challenges, each designed to familiarize the scientific community with SKAO data and to drive the development of new analysis techniques. We present the results from Science Data Challenge 2 (SDC2), which invited participants to find and characterize 233 245 neutral hydrogen (H I) sources in a simulated data product representing a 2000 h SKA-Mid spectral line observation from redshifts 0.25–0.5. Through the generous support of eight international supercomputing facilities, participants were able to undertake the Challenge using dedicated computational resources. Alongside the main challenge, ‘reproducibility awards’ were made in recognition of those pipelines which demonstrated Open Science best practice. The Challenge saw over 100 participants develop a range of new and existing techniques, with results that highlight the strengths of multidisciplinary and collaborative effort. The winning strategy – which combined predictions from two independent machine learning techniques to yield a 20 per cent improvement in overall performance – underscores one of the main Challenge outcomes: that of method complementarity. It is likely that the combination of methods in a so-called ensemble approach will be key to exploiting very large astronomical data sets.This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no. 679627; project name FORNAX). JMvdH and KMH acknowledge support from the European Research Council under the European Union 7th Framework Programme (FP/2007–2013)/ERC Grant Agreement no. 291531 (HIStoryNU). SSI. The works of the NAOC-Tianlai team members have been supported by the National Key R&D Program grants 2018YFE0120800,2017YFA0402603, 2018YFA0404504, 2018YFA9494691, The National Natural Science Foundation of China (NSFC) grants 11633004, 11975072, 11835009, 11890691, 12033008, the Chinese Academy of Science (CAS) QYZDJ-SSW-SLH017, JCTD-2019-05, and the China Manned Space Projects CMS-CSST-2021-A03, CMS-CSST-2021-B01. Team FORSKA-Sweden acknowledges support from Onsala Space Observatory for the provisioning of its facilities support. The Onsala Space Observatory national research infrastructure is funded through Swedish Research Council (grant No. 2017–00648). Team FORSKA-Sweden also acknowledges support from the Fraunhofer Cluster of Excellence Cognitive Internet Technologies. CH, MB acknowledge support by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2121 ‘Quantum Universe’ – 390833306. MP acknowledges the support of the CEFIPRA foundation under project 6504–3. We acknowledge financial support from SEV-2017-0709, CEX2021-001131-S, AEI/ 10.13039/501100011033. LD, JG, KMH, JM, MP, SSE, LVM, AS from RTI2018-096228-B-C31, PID2021-123930OB-C21 AEI/ 10.13039/501100011033 FEDER, UE. LVM, JG, SSE acknowledge The European Science Cluster of Astronomy and Particle Physics ESFRI Research Infrastructures project that has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No. 824064. LVM, JG, and JM RED2018-102587-T AEI/ 10.13039/501100011033. LVM, JG, SSE, JM acknowledge financial support from the grant IAA4SKA (Ref. R18-RT-3082) from the Economic Transformation, Industry, Knowledge and Universities Council of the Regional Government of Andalusia and the ERDF from the EU, TED2021-130231B-I00 AEI/ 10.13039/501100011033 EU NextGenerationEU/PRTR. LVM, JG, KMH acknowledges financial support from the coordination of the participation in SKA-SPAIN, funded by the Ministry of Science and Innovation (MCIN). LD from PTA2018-015980-I AEI/ 10.13039/501100011033. MP from the grant DOC01497 funded by the Economic Transformation, Industry, Knowledge and Universities Council of the Regional Government of Andalusia and by the Operational Program ESF Andalucía 2014–2020. MTS acknowledges support from a Scientific Exchanges visitor fellowship (IZSEZO_202357) from the Swiss National Science Foundation.With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2021-001131-S).Peer reviewe