ANALYSIS OF INFLUENCE OF BIOHUMUS ON THE BASIS OF CONSORTIUM OF EFFECTIVE MICROORGANISMS ON THE PRODUCTIVITY OF WINTER WHEAT

The article considers the problem of recycling of poultry waste, in particular, feather-downy waste and chicken dung. Traditional ways of poultry waste disposal have been shown and their shortcomings have been described. The main advantages of use of bioprocessing methods to implement the process of waste conversion with the formation of effective products - biohumus - have been determined. Biofertilizers or biohumus is a modern innovative means applied not only for the purpose of an increase in productivity, but also for land recultivation and resuscitation. The authors have studied the biohumus obtained by means of processing of mixture of feather-downy waste and dung in the ratio of 8 : 2 with the application of the biological product containing the consortium of decomposer microorganisms: Bacillus pumilus AL16, Microbacterium terregens AC1180, Aeromonas sp. B5376, Arthrobacter globiformis AC1529, Streptomyces olivocinereus AC1169 and Acinetobacter sp. B390. An assessment of efficiency of application of various rates of the developed biohumus for winter wheat crops has been made. It isshown that the application of a comparison sample - the organic mineral fertilizer "Universal'noe" (the expense is 150 g/m2) and the use of the developed biohumus (the expense is 150 g/m2) provided an increase in its growth, compared with the control sample of the wheat '"Skipetr", by 12 and 15 cm (the stem elongation stage), by 15 and 18 cm (the earing stage), by 21 and 30 cm (the milk stage) and by 19 and 30 cm (the firm ripe stage) respectively. The above values for the grade Zimushka were within the similar limits: an increase in its growth, compared with the control sample, by 17 and 18 cm (the stem elongation stage), by 19 and 21 cm (the earing stage) and by 19 and 31 cm (the milk stage and the firm ripe stage) respectively.An increase in the productivity of grades of the winter wheat "Skipetr" and "Zimushka" by 1.5 times (1.25 ± 0.25 t/hectare) has been established during the application of the obtained biohumus with the rate of 150 g/m2. The results of studies of change of fractional composition of grain proteins testify that the application of biohumus provides an increase in gluten fractions of winter wheat grains. An assessment of economic efficiency has been performed, it has been established that the highest rates of profitability refer to the variant with the application of the developed biohumus (the expense is 150 g/m2) - 63.2%, the lowest rates are 39.2% in the control sample

STUDY OF THE BIOFUNCTIONAL PROPERTIES OF CEDAR PINE OIL WITH THE USE OF TESTING CULTURES

Cedar pine ( Pinus sibirica ) nuts are an environmentally friendly natural product that contains a unique set of useful biologically active substances. Due to its composition, pine nuts and their derivative products are widely used in a comprehensive therapy and prevention program for a lot of diseases. The objects of the study were cedar oil and oil emulsions (the cedar oil concentration was 1.0, 5.0 and 10.0%). The antimicrobial properties were determined using the diffusion method and by measuring the optical density. The prebiotic properties were estimated according to the ability to stimulate the bifidobacteria growth. The antioxidant activity was determined using the fluorescent ORAC method. The antihypertensive activity was estimated according to the ability to inhibit the angiotensin-1-converting enzyme. All the studied experimental oil emulsion samples, regardless of a pressing method (cold or heat), showed high antimicrobial characteristics without suppressing only Candida albicans EMTC 34 and Proteus vulgaris ATCC 63 from the studied 10 strains of the main testing cultures. The prebiotic properties of the emulsions obtained with the addition of cedar oil have been determined. The number of cells of the bifidobacteria cultivated in nutrient media with the addition of cedar oil (with the concentration from 5.0%) is almost 3 times as large as the amount when cultivated without it. Antioxidant cedar oil properties have been revealed. The hypotensive characteristics of cedar oil can be observed even at a concentration of 5.0%, the percentage of inhibition of the angiotensin-1-converting enzyme is up to 69%. With an increase in the concentration of cedar oil to 10.0% inhibition increases to 70%. The carried out studies of the functional properties of experimental cedar oil samples have confirmed its high quality and an opportunity to use it as the basis of biologically active food supplements and dietary, medical-preventive and sports nutrition