Defining Young in the Context of Prostate Cancer

The experience of prostate cancer is for most men a major life stress with the psychological burden of this disease falling more heavily on those who are younger. Despite this, being young as it applies to prostate cancer is not yet clearly defined with varied chronological approaches applied. However, men’s responses to health crises are closely bound to life course and masculinities from which social roles emerge. This paper applied qualitative methodology (structured focus groups and semistructured interviews with expert informants) using interpretative phenomenological analysis to define what it means to be young and have prostate cancer. Structured focus groups were held with 26 consumer advisors (men diagnosed with prostate cancer who provide support to other men with prostate cancer or raise community awareness) and health professionals. As well, 15 men diagnosed with prostate cancer and in their 40s, 50s, or 60s participated in semi-structured interviews. Participants discussed the attributes that describe a young man with prostate cancer and the experience of being young and diagnosed with prostate cancer. Chronological definitions of a young man were absent or inconsistent. Masculine constructions of what it means to be a young man and life course characteristics appear more relevant to defining young as it applies to prostate cancer compared with chronological age. These findings have implications for better understanding the morbidities associated with this illness, and in designing interventions that are oriented to life course and helping young men reconstruct their identities after prostate cancer

Cat States and Single Runs for the Damped Harmonic Oscillator

We discuss the fate of initial states of the cat type for the damped harmonic oscillator, mostly employing a linear version of the stochastic Schr\"odinger equation. We also comment on how such cat states might be prepared and on the relation of single realizations of the noise to single runs of experiments.Comment: 18, Revte

Pumped-Up SU(1,1) interferometry

Although SU(1,1) interferometry achieves Heisenberg-limited sensitivities, it suffers from one major drawback: Only those particles outcoupled from the pump mode contribute to the phase measurement. Since the number of particles outcoupled to these “side modes” is typically small, this limits the interferometer’s absolute sensitivity. We propose an alternative “pumped-up” approach where all the input particles participate in the phase measurement and show how this can be implemented in spinor Bose-Einstein condensates and hybrid atom-light systems—both of which have experimentally realized SU(1,1) interferometry. We demonstrate that pumped-up schemes are capable of surpassing the shot-noise limit with respect to the total number of input particles and are never worse than conventional SU(1,1) interferometry. Finally, we show that pumped-up schemes continue to excel—both absolutely and in comparison to conventional SU(1,1) interferometry—in the presence of particle losses, poor particle-resolution detection, and noise on the relative phase difference between the two side modes. Pumped-up SU(1,1) interferometry therefore pushes the advantages of conventional SU(1,1) interferometry into the regime of high absolute sensitivity, which is a necessary condition for useful quantum-enhanced devices

Seminal plasma enables selection and monitoring of active surveillance candidates using nuclear magnetic resonance-based metabolomics: A preliminary investigation

Background: Diagnosis and monitoring of localized prostate cancer requires discovery and validation of noninvasive biomarkers. Nuclear magnetic resonance (NMR)-based metabolomics of seminal plasma reportedly improves diagnostic accuracy, but requires validation in a high-risk clinical cohort. Materials and methods: Seminal plasma samples of 151 men being investigated for prostate cancer were analyzed with 1H-NMR spectroscopy. After adjustment for buffer (add-to-subtract) and endogenous enzyme influence on metabolites, metabolite profiling was performed with multivariate statistical analysis (principal components analysis, partial least squares) and targeted quantitation. Results: Seminal plasma metabolites best predicted low- and intermediate-risk prostate cancer with differences observed between these groups and benign samples. Lipids/lipoproteins dominated spectra of high grade samples with less metabolite contributions. Overall prostate cancer prediction using previously described metabolites was not validated. Conclusion: Metabolomics of seminal plasma in vitro may assist urologists with diagnosis and monitoring of either low or intermediate grade prostate cancer. Less clinical benefit may be observed for high-risk patients. Further investigation in active surveillance cohorts, and/or in combination with in vivo magnetic resonance spectroscopic imaging may further optimize localized prostate cancer outcomes

New Genomic Structure for Prostate Cancer Specific Gene PCA3 within BMCC1: Implications for Prostate Cancer Detection and Progression

The prostate cancer antigen 3 (PCA3/DD3) gene is a highly specific biomarker upregulated in prostate cancer (PCa). In order to understand the importance of PCA3 in PCa we investigated the organization and evolution of the PCA3 gene locus. METHODS/PRINCIPAL FINDINGS: We have employed cDNA synthesis, RTPCR and DNA sequencing to identify 4 new transcription start sites, 4 polyadenylation sites and 2 new differentially spliced exons in an extended form of PCA3. Primers designed from these novel PCA3 exons greatly improve RT-PCR based discrimination between PCa, PCa metastases and BPH specimens. Comparative genomic analyses demonstrated that PCA3 has only recently evolved in an anti-sense orientation within a second gene, BMCC1/PRUNE2. BMCC1 has been shown previously to interact with RhoA and RhoC, determinants of cellular transformation and metastasis, respectively. Using RT-PCR we demonstrated that the longer BMCC1-1 isoform - like PCA3 - is upregulated in PCa tissues and metastases and in PCa cell lines. Furthermore PCA3 and BMCC1-1 levels are responsive to dihydrotestosterone treatment. CONCLUSIONS/SIGNIFICANCE: Upregulation of two new PCA3 isoforms in PCa tissues improves discrimination between PCa and BPH. The functional relevance of this specificity is now of particular interest given PCA3's overlapping association with a second gene BMCC1, a regulator of Rho signalling. Upregulation of PCA3 and BMCC1 in PCa has potential for improved diagnosis

Regulation of platelet activation and thrombus formation by reactive oxygenspecies

Reactive oxygen species (ROS) are generated within activated platelets and play an important role in regulating platelet responses to collagen and collagen-mediated thrombus formation. As a major collagen receptor, plateletspecific glycoprotein (GP)VI is a member of the immunoglobulin (Ig) superfamily, with two extracellular Ig domains, a mucin domain, a transmembrane domain and a cytoplasmic tail. GPVI forms a functional complex with the Fc receptor γ-chain (FcRγ) that, following receptor dimerization, signals via an intracellular immunoreceptor tyrosine-based activation motif (ITAM), leading to rapid activation of Src family kinase signaling pathways. Our previous studies demonstrated that an unpaired thiol in the cytoplasmic tail of GPVI undergoes rapid oxidation to form GPVI homodimers in response to ligand binding, indicating an oxidative submembranous environment in platelets after GPVI stimulation. Using a redox-sensitive fluorescent dye (H2DCFDA) in a flow cytometric assay to measure changes in intracellular ROS, we showed generation of ROS downstream of GPVI consists of two distinct phases: an initial Syk-independent burst followed by additional Sykdependent generation. In this review, we will discuss recent findings on the regulation of platelet function by ROS, focusing on GPVI-dependent platelet activation and thrombus formation.This research was supported by National Natural Science Foundation of China (grant no. 81400082, 81370602 and 81570096), the Natural Science Foundation of Jiangsu Province (grant no. BK20140219), the funding for the Distinguished Professorship Program of Jiangsu Province, the Six Talent Peaks Project of Jiangsu Province (WSN-133), the Shuangchuang Project of Jiangsu Province, the 333 Project of Jiangsu Province (BRA2017542), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, the Science and Technology Foundation for the Selected Overseas Chinese Scholars, State Ministry of Human Resources and Social Security, and the National Health and Medical Research Council of Australi

A randomised trial of robotic and open prostatectomy in men with localised prostate cancer

Background: Prostate cancer is the most common male cancer in the Western world however there is ongoing debate about the optimal treatment strategy for localised disease. While surgery remains the most commonly received treatment for localised disease in Australia more recently a robotic approach has emerged as an alternative to open and laparoscopic surgery. However, high level data is not yet available to support this as a superior approach or to guide treatment decision making between the alternatives. This paper presents the design of a randomised trial of Robotic and Open Prostatectomy for men newly diagnosed with localised prostate cancer that seeks to answer this question.Methods/design: 200 men per treatment arm (400 men in total) are being recruited after diagnosis and before treatment through a major public hospital outpatient clinic and randomised to 1) Robotic Prostatectomy or 2) Open Prostatectomy. All robotic prostatectomies are being performed by one surgeon and all open prostatectomies are being performed by one other surgeon. Outcomes are being measured pre-operatively and at 6 weeks and 3, 6, 12 and 24 months post-surgery. Oncological outcomes are being related to positive surgical margins, biochemical recurrence +/- the need for further treatment. Non-oncological outcome measures include: pain, physical and mental functioning, fatigue, summary (preference-based utility scores) and domain-specific QoL (urinary incontinence, bowel function and erectile function), cancer specific distress, psychological distress, decision-related distress and time to return to usual activities. Cost modelling of each approach, as well as full economic appraisal, is also being undertaken.Discussion: The study will provide recommendations about the relative benefits of Robotic and Open Prostatectomy to support informed patient decision making about treatment for localised prostate cancer; and to assist in treatment services planning for this patient group.Trial registration: ACTRN12611000661976

Condensate fluctuations in finite Bose-Einstein condensates at finite temperature

A Langevin equation for the complex amplitude of a single-mode Bose-Einstein condensate is derived. The equation is first formulated phenomenologically, defining three transport parameters. It is then also derived microscopically. Expressions for the transport parameters in the form of Green-Kubo formulas are thereby derived and evaluated for simple trap geometries, a cubic box with cyclic boundary conditions and an isotropic parabolic trap. The number fluctuations in the condensate, their correlation time, and the temperature-dependent collapse-time of the order parameter as well as its phase-diffusion coefficient are calculated.Comment: 29 pages, Revtex, to appear in Phys.Rev.

Macroscopic superpositions of Bose-Einstein condensates

We consider two dilute gas Bose-Einstein condensates with opposite velocities from which a monochromatic light field detuned far from the resonance of the optical transition is coherently scattered. In the thermodynamic limit, when the relative fluctuations of the atom number difference between the two condensates vanish, the relative phase between the Bose-Einstein condensates may be established in a superposition state by detections of spontaneously scattered photons, even though the condensates have initially well-defined atom numbers. For a finite system, stochastic simulations show that the measurements of the scattered photons lead to a randomly drifting relative phase and drive the condensates into entangled superpositions of number states. This is because according to Bose-Einstein statistics the scattering to an already occupied state is enhanced.Comment: 18 pages, RevTex, 5 postscript figures, 1 MacBinary eps-figur