Development of microfluidic devices with 3D collagen gels for traction force microscopy

In the past few decades, it has been widely demonstrated that cells constantly exert traction forces on the cells and matrix surrounding them. This behavior is fundamental in physiological and pathological processes such as embryogenesis and metastasis, therefore studying how cells interact with their environment is vitally important to understand these processes. To contribute to this research field, the present master project aims to adapt a given microfluidic device to the traction force microscopy technique, which is currently the most reliable approach for measuring cell forces. It is of great interest to quantify the forces exerted by cells seeded inside this microfluidic device because this specific device allows the deposition of collagen in a 3D arrangement, which resembles better the cell’s physiological environment than traditional 2D cell cultures. Device adaptation consisted in introduction of fluorescent microbeads into the collagen matrix, and subsequent confocal microscopy imaging of the cultured cells. Both stages required optimization of diverse features, for example: bead size and concentration, cell viability and labeling, fluorescence staining complications, etc. After numerous experiments and information search, the aforementioned features were improved, and ultimately, the microfluidic device was successfully adapted to traction force microscopy. Thus, the final assays produced useful data for performing the cell force calculation

Virtual Reality mobile technology in education: a review of the state of scientific literature in Spain

La Realidad Virtual es una de las herramientas tecnológicas emergentes y su tendencia va en aumento en relación a su aplicación en el ámbito educativo. El presente escrito forma parte de una investigación más amplia sobre la aplicación de la metodología mobile learning en la Universidad española. En concreto en este trabajo se recoge la literatura científica sobre Realidad Virtual aplicada a través de los dispositivos digitales móviles en España entre los años 2000 y finales de 2017. Se ha seguido una metodología propia de la investigación bibliográfica que ha consistido en el establecimiento de una serie de palabras clave y criterios para la realización de la búsqueda sistemática de documentación en bases de datos científicas. Entre los resultados obtenidos se ha determinado el creciente interés a partir del año 2015, siendo la mayor parte de los documentos publicados artículos de revista. Así pues, se constata el auge en la implementación de la Realidad Virtual en diferentes niveles y ámbitos educativos gracias a la universalización del Smartphone y el abaratamiento de los visores de Realidad Virtual.Virtual Reality is one of the emerging technological tools and its tendency is increasing in relation to its application in the educational field. This paper takes part of a wider investigation on the application of the mobile learning methodology in the Spanish University. Specifically in this work is collected the scientific literature on Virtual Reality applied through mobile digital devices in Spain between years 2000 and the end of 2017. It has followed a methodology of bibliographic research that has consisted in the establishment of a series of keywords and criteria for carrying out the systematic search of documentation in scientific databases. Among the results obtained, it shows that the growing interest has been determined since 2015, with most of the documents published being journal articles. Therefore, the boom in the implementation of the Virtual Reality in different levels and educational areas is verified thanks to the universalization of the Smartphone and the reduction of the Virtual Reality viewers

Memoria de la red de coordinación del tercer curso del grado en Ingeniería Multimedia

Durante el presente curso se ha constatado el asentamiento del tercer curso del grado en Ingeniería Multimedia, lo que se deriva de los informes de seguimiento de las asignaturas del curso que, en su gran mayoría, no han destacado problema alguno (con alguna excepción de la que se informa). Por otro lado, se ha llevado a cabo una iniciativa para contrastar si las dependencias entre las asignaturas de tercero con respecto a sus precedentes en el plan de estudios responden en realidad a los planteamientos que se hicieron durante el diseño del mismo, intentando descubrir carencias o inconsistencias en los contenidos. De esta manera, se han detectado dependencias que no son tales, dependencias que faltan y temarios de asignaturas básicas en los que, desde el punto de vista de las asignaturas de tercero faltan o sobran contenidos

Análisis biomecánico de la epifisiolisis de la cabeza femoral: comparación entre fémures sanos y enfermos

En este trabajo se desarrolla un análisis por elementos finitos cuyo objetivo principal es determinar las diferencias de tensiones en la placa de crecimiento que se producen entre fémures sanos, con epifisiolisis unilateral y con epifisiolisis bilateral, para evaluar sus posibles causas. Se elaboraron los modelos de elementos finitos correspondientes a 45 pacientes. Los resultados mostraron un patrón de esfuerzos similar en todos los grupos de fémures y, además, la aparición de tensiones mayores en el grupo con epifisiolisis con respecto al grupo control. Se observó también que el valor del ángulo axial-fisis dependía significativamente del tipo de fémur analizado, y, además, una mayor influencia de los factores geométricos en la incidencia de la enfermedad, en comparación con la del índice de masa corporal.In this work, a finite element analysis (FEA) is accomplished to study the differences of stresses in the growth plate, that are produced in healthy and unhealthy femurs, and to evaluate the possible causes of this illness. Finite element models of 45 patients were developed. The results demonstrated a similar pattern of stresses in all the groups of femurs and also the appearance of greater stresses in the group with slipped capital femoral epiphysis than in the control group. It was also observed a strong dependency on the value of the axial-fisis angle from the group of femur analyzed and a bigger influence of the geometric factors than of the body mass index, in the incidence of the illness

Characterization of mutant versions of the R-RAS2/TC21 GTPase found in tumors

The R-RAS2 GTP hydrolase (GTPase) (also known as TC21) has been traditionally considered quite similar to classical RAS proteins at the regulatory and signaling levels. Recently, a long-tail hotspot mutation targeting the R-RAS2/TC21 Gln72 residue (Q72L) was identified as a potent oncogenic driver. Additional point mutations were also found in other tumors at low frequencies. Despite this, little information is available regarding the transforming role of these mutant versions and their relevance for the tumorigenic properties of already-transformed cancer cells. Here, we report that many of the RRAS2 mutations found in human cancers are highly transforming when expressed in immortalized cell lines. Moreover, the expression of endogenous R-RAS2Q72L is important for maintaining optimal levels of PI3K and ERK activities as well as for the adhesion, invasiveness, proliferation, and mitochondrial respiration of ovarian and breast cancer cell lines. Endogenous R-RAS2Q72L also regulates gene expression programs linked to both cell adhesion and inflammatory/immune-related responses. Endogenous R-RAS2Q72L is also quite relevant for the in vivo tumorigenic activity of these cells. This dependency is observed even though these cancer cell lines bear concurrent gain-of-function mutations in genes encoding RAS signaling elements. Finally, we show that endogenous R-RAS2, unlike the case of classical RAS proteins, specifically localizes in focal adhesions. Collectively, these results indicate that gain-of-function mutations of R-RAS2/TC21 play roles in tumor initiation and maintenance that are not fully redundant with those regulated by classical RAS oncoproteins

Targeting hypersialylation in pancreatic ductal adenocarcinoma to reverts their malignant phenotype

Trabajo presentado en el 15th Jenner Glycobiology and Medicine Symposium, celebrado en Oporto (Portugal), del 14 al 16 de junio de 202

Evaluación de la implantación transversal de 3º del grado en Ingeniera Multimedia

Durante el presente curso se han llevado a cabo las tareas para la evaluación de la implantación del grado en Ingeniería Multimedia en el marco de los nuevos estudios dentro del EEES, lo cual implica un trabajo extenso de análisis y recopilación del tratamiento concreto que se está dando a la adquisición por parte del alumnado de las diferentes competencias planteadas para cada asignatura en su ficha correspondiente y en la memoria para la solicitud de la verificación del título. Esto ha permitido tener una visión global de cuáles son los medios que se ponen para evaluar dichas competencias y también, desde un punto de vista más cercano, qué calendario de evaluación “ve” el alumno. En esta memoria nos hemos centrado en los aspectos relacionados con el curso tercero del grado

Novel frameshift variants expand the map of the genetic defects in IRF2BP2

BackgroundAt present, the knowledge about disease-causing mutations in IRF2BP2 is very limited because only a few patients affected by this condition have been reported. As previous studies have described, the haploinsufficiency of this interferon transcriptional corepressors leads to the development of CVID. Very recently, a more accurate phenotype produced by truncating variants in this gene has been defined, manifesting CVID with gastrointestinal inflammatory symptoms and autoimmune manifestations.MethodsWe analyzed 5 index cases with suspected primary immunodeficiency by high throughput sequencing. They were submitted for a genetic test with a panel of genes associated with immune system diseases, including IRF2BP2. The screening of SNVs, indels and CNVs fulfilling the criteria with very low allelic frequency and high protein impact, revealed five novel variants in IRF2BP2. In addition, we isolated both wild-type and mutated allele of the cDNA from one of the families.ResultsIn this study, we report five novel loss-of-function (LoF) mutations in IRF2BP2 that likely cause primary immunodeficiency, with CVID as more frequent phenotype, variable expression of inflammatory gastrointestinal features, and one patient with predisposition of viral infection. All identified variants were frameshift changes, and one of them was a large deletion located on chromosome 1q42, which includes the whole sequence of IRF2BP2, among other genes. Both de novo and dominant modes of inheritance were observed in the families here presented, as well as incomplete penetrance.ConclusionsWe describe novel variants in a delimited low-complex region, which may be considered a hotspot in IRF2BP2. Moreover, this is the first time that a large CNV in IRF2BP2 has been reported to cause CVID. The distinct mechanisms than LoF in IRF2BP2 could cause different phenotype compared with the mainly described. Further investigations are necessary to comprehend the regulatory mechanisms of IRF2BP2, which could be under variable expression of the disease

Safety and Revisit Related to Discharge the Sixty-one Spanish Emergency Department Medical Centers Without Hospitalization in Patients with COVID-19 Pneumonia. A Prospective Cohort Study UMC-Pneumonia COVID-19

Background: Information is needed on the safety and efficacy of direct discharge from the emergency department (ED) of patients with COVID-19 pneumonia. Objectives: The objectives of the study were to study the variables associated with discharge from the ED in patients presenting with COVID-19 pneumonia, and study ED revisits related to COVID-19 at 30 days (EDR30d). Methods: Multicenter study of the SIESTA cohort including 1198 randomly selected COVID patients in 61 EDs of Spanish medical centers from March 1, 2020, to April 30, 2020. We collected baseline and related characteristics of the acute episode and calculated the adjusted odds ratios (aOR) for ED discharge. In addition, we analyzed the variables related to EDR30d in discharged patients. Results: We analyzed 859 patients presenting with COVID-19 pneumonia, 84 (9.8%) of whom were discharged from the ED. The variables independently associated with discharge were being a woman (aOR 1.890; 95%CI 1.176-3.037), age 1200/mm(3) (aOR 4.667; 95%CI 1.045-20.839). The EDR30d of the ED discharged group was 40.0%, being lower in women (aOR 0.368; 95%CI 0.142-0.953). A total of 130 hospitalized patients died (16.8%) as did two in the group discharged from the ED (2.4%) (OR 0.121; 95%CI 0.029-0.498). Conclusion: Discharge from the ED in patients with COVID-19 pneumonia was infrequent and was associated with few variables of the episode. The EDR30d was high, albeit with a low mortality