Efficiency of an inexpensive liquid-based cytology performed by cytocentrifugations: a comparative study using the histology as reference standard

BACKGROUND: Although liquid-based cytology (LBC) is now recommended for cervical cancer screening, it requires expensive automated devices and materials. To evaluate the efficiency of inexpensive LBC methods relying on an inexpensive fixative liquid, Easyfix(®), we compared the results obtained by the liquid-based cytology (LBC) diagnoses performed by cytocentrifugations (Papspin(® )and Turbitec(®)) with those obtained by histology. Furthermore, we evaluated the efficiency of the fixative liquid, Easyfix(®), to preserve HPV DNA in the collected samples. METHOD: 266 LBC were compared with 174 colposcopies and 91 Loop Electrosurgical Excision Procedure (LEEP). Among the LBC, 51 were performed using the Papspin(® )system and 215 were performed using the Turbitec(® )system. To control the quality of the preservation liquid, Easyfix(®), we correlated the results of HCII assays with those of HPV PCR. RESULTS: For Papspin(® )and Turbitec(® )systems, the sensitivities were respectively 82.6% (95% CI: 61.2–95.0%, p < 0.001) and 75.0% (95% CI: 64.4–89.8%, p < 0.001) and the specificities were 92.6% (95%CI: 76.5–99.1%, p < 0.001) and 96.2% (95% CI: 91.3–98.7%, p < 0.001). We find no statistical difference between the results of the both systems (p = ns). The sensitivity of the HCII was 86.4% (95% IC: 77.4–92.8%, p < 0.001) and the specificity was 39.4% (95% CI: 31.2–48.1%, p < 0.001). The comparison between HCII and HPV-PCR shows a good correlation: the kappa was 0.89. CONCLUSION: LBC performed by cytocentrifugations are inexpensive, reduce inadequate smears, show excellent efficiency and allow HPV detection by molecular biology

Effect of streptozotocin-induced diabetes on myocardial blood flow reserve assessed by myocardial contrast echocardiography in rats

The role of structural and functional abnormalities of small vessels in diabetes cardiomyopathy remains unclear. Myocardial contrast echocardiography allows the quantification of myocardial blood flow at rest and during dipyridamole infusion. The aim of the study was to determine the myocardial blood flow reserve in normal rats compared with Streptozotocin-induced diabetic rats using contrast echocardiography

L’annulation de l’accord EDF-GDF sur les 32 heures

International audienc

Présentation

Garbar Christian. Présentation. In: Revue internationale de droit comparé. Vol. 68 N°1,2016. pp. 7-9

La contractualisation de l’action publique

National audienc

Entreprises publiques et transparence

International audienc

Le droit social vu du droit public : un lieu d’hybridation

International audienc