3 research outputs found
ΠΠ»ΠΈΡΠ½ΠΈΠ΅ Π»ΠΎΠΊΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΎΡΡΠΈΠΊΠΎΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΈ ΡΠ°ΠΊΡΠΎΠ»ΠΈΠΌΡΡΠ° Π½Π° ΠΌΠΎΡΡΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΊΠΎΠΆΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ ΡΠΎΡΠΎΡΠΈΠΏΠΎΠ²
Objective. To assess the effect of local corticosteroid therapy and Tacrolimus on the morphological and functional skin indices of different phototypes. Materials and methods. The authors conducted a complex assessment of the morphological and functional skin condition of different phototypes among healthy volunteers under the impact of corticosteroids and Tacrolimus. The study involved 20 healthy volunteers (10 people of the 1st and 2nd skin phototypes and 10 people of the 5th and 6th skin phototypes; 14 males and 6 females; mean age: 2Π· years). The morphological condition of the skin was assessed with the use of optical coherent tomography (Applied Physics Institute, Russian Academy of Sciences, Nizhny Novgorod) while the functional condition was assessed with the use of Multi Skin Test Center MC 750 (Courage+Khazaka Cologne, Germany). Results. The authors revealed that the application of local corticosteroids is accompanied with a reliable reduction in the epidermis thickness developing earlier in the subjects belonging to the 5th and 6th phototypes and, at the same time, increased transepidermal water loss and reduced corneous layer humidity. An increase in the corneous layer thickness accompanied by an increase in the humidity factor was registered in all phototypes in the areas where Tacrolimus was applied. It is possible to select the correct tactics of the anti-inflammatory therapy on the basis of monitoring the morphological and functional skin condition in the process of applying local corticosteroids: to determine the optimum duration of the corticosteroid therapy and switch to calcineurin inhibitors on a timely basis when there is a need to perform an antiinflammatory therapy to achieve the maximum efficacy with the minimum risk of complication development.Π¦Π΅Π»Ρ. ΠΡΠ΅Π½ΠΈΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Π»ΠΎΠΊΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΎΡΡΠΈΠΊΠΎΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΈ ΡΠ°ΠΊΡΠΎΠ»ΠΈΠΌΡΡΠ° Π½Π° ΠΌΠΎΡΡΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΠΊΠΎΠΆΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΎΡΠΎΡΠΈΠΏΠΎΠ². ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½Π°Ρ ΠΎΡΠ΅Π½ΠΊΠ° ΠΌΠΎΡΡΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΊΠΎΠΆΠΈ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄ΠΎΠ±ΡΠΎΠ²ΠΎΠ»ΡΡΠ΅Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠΎΡΠΎΡΠΈΠΏΠΎΠ² ΠΏΠΎΠ΄ Π²Π»ΠΈΡΠ½ΠΈΠ΅ΠΌ ΠΊΠΎΡΡΠΈΠΊΠΎΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΡΡ
ΡΡΠ΅Π΄ΡΡΠ² ΠΈ ΡΠ°ΠΊΡΠΎΠ»ΠΈΠΌΡΡΠ°. Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΡΠΈΠ½ΡΠ»ΠΈ ΡΡΠ°ΡΡΠΈΠ΅ 20 Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄ΠΎΠ±ΡΠΎΠ²ΠΎΠ»ΡΡΠ΅Π² (10 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ I, II ΡΠΎΡΠΎΡΠΈΠΏΠΎΠ² ΠΊΠΎΠΆΠΈ ΠΈ 10 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ V, VI ΡΠΎΡΠΎΡΠΈΠΏΠΎΠ²; 14 ΠΌΡΠΆΡΠΈΠ½ ΠΈ 6 ΠΆΠ΅Π½ΡΠΈΠ½, ΡΡΠ΅Π΄Π½ΠΈΠΉ Π²ΠΎΠ·ΡΠ°ΡΡ 23 Π³ΠΎΠ΄Π°). ΠΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ ΠΊΠΎΠΆΠΈ ΠΈΠ·ΡΡΠ°Π»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΎΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΊΠΎΠ³Π΅ΡΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠ° (ΠΠΠ€ Π ΠΠ, ΠΠΈΠΆΠ½ΠΈΠΉ ΠΠΎΠ²Π³ΠΎΡΠΎΠ΄), ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠ΅ β Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΌΠ½ΠΎΠ³ΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠ±Π°ΠΉΠ½Π° Multi Skin Test Center MC 750 (βCourage + Khazaka Cologneβ, ΠΠ΅ΡΠΌΠ°Π½ΠΈΡ). Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π½Π°Π½Π΅ΡΠ΅Π½ΠΈΠ΅ Π»ΠΎΠΊΠ°Π»ΡΠ½ΡΡ
ΠΊΠΎΡΡΠΈΠΊΠΎΡΡΠ΅ΡΠΎΠΈΠ΄ΠΎΠ² ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°Π΅ΡΡΡ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΡΠΌ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΠΎΠ»ΡΠΈΠ½Ρ ΡΠΏΠΈΠ΄Π΅ΡΠΌΠΈΡΠ°, ΡΠ°Π·Π²ΠΈΠ²Π°ΡΡΠΈΠΌΡΡ Π² Π±ΠΎΠ»Π΅Π΅ ΡΠ°Π½Π½ΠΈΠ΅ ΡΡΠΎΠΊΠΈ Ρ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΉ V, VI ΡΠΎΡΠΎΡΠΈΠΏΠΎΠ², ΠΈ ΠΏΠ°ΡΠ°Π»Π»Π΅Π»ΡΠ½ΠΎ Π½Π°Π±Π»ΡΠ΄Π°ΡΡΠΈΠΌΡΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ ΡΡΠ°Π½ΡΡΠΏΠΈΠ΄Π΅ΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΠΏΠΎΡΠ΅ΡΠΈ Π²ΠΎΠ΄Ρ ΠΈ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ Π²Π»Π°ΠΆΠ½ΠΎΡΡΠΈ ΡΠΎΠ³ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ»ΠΎΡ. Π Π·ΠΎΠ½Π΅ Π½Π°Π½Π΅ΡΠ΅Π½ΠΈΡ ΡΠ°ΠΊΡΠΎΠ»ΠΈΠΌΡΡΠ° Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΠΎΠ»ΡΠΈΠ½Ρ ΡΠΎΠ³ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ»ΠΎΡ Ρ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΉ Π²ΡΠ΅Ρ
ΡΠΎΡΠΎΡΠΈΠΏΠΎΠ², ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°ΡΡΠ΅Π΅ΡΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ Π²Π»Π°ΠΆΠ½ΠΎΡΡΠΈ. ΠΠΎΠ½ΠΈΡΠΎΡΠΈΠ½Π³ ΠΌΠΎΡΡΠΎΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΊΠΎΠΆΠΈ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π»ΠΎΠΊΠ°Π»ΡΠ½ΡΡ
ΠΊΠΎΡΡΠΈΠΊΠΎΡΡΠ΅ΡΠΎΠΈΠ΄ΠΎΠ² ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΏΡΠ°Π²ΠΈΠ»ΡΠ½ΠΎ Π²ΡΠ±ΡΠ°ΡΡ ΡΠ°ΠΊΡΠΈΠΊΡ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ: ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΡ ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΊΠΎΡΡΠΈΠΊΠΎΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΈ ΠΏΡΠΈ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΠΈ ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠ΅Π½ΠΈΡ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΎΡΡΡΠ΅ΡΡΠ²ΠΈΡΡ ΡΠ²ΠΎΠ΅Π²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠΉ ΠΏΠ΅ΡΠ΅Ρ
ΠΎΠ΄ Π½Π° ΠΈΠ½Π³ΠΈΠ±ΠΈΡΠΎΡΡ ΠΊΠ°Π»ΡΡΠΈΠ½Π΅Π²ΡΠΈΠ½Π° ΠΈ ΡΠ°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ Π΄ΠΎΠ±ΠΈΡΡΡΡ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Ρ ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½ΡΠΌ ΡΠΈΡΠΊΠΎΠΌ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ
Oviduct fluid extracellular vesicles regulate polyspermy during porcine in vitro fertilisation
International audienceHigh polyspermy is one of the major limitations of porcine in vitro fertilisation (IVF). The addition of oviductal fluid (OF) during IVF reduces polyspermy without decreasing the fertilisation rate. Because extracellular vesicles (EVs) have been described as important OF components, the aim of this study was to evaluate the effect of porcine oviductal EVs (poEVs) on IVF efficiency compared with porcine OF (fresh and lyophilised). OF was collected from abattoir oviducts by phosphate-buffered saline flush, and poEVs were isolated by serial ultracentrifugation. Four IVF treatments were conducted: poEVs (0.2 mg mL(-1)), OF (10%), lyophilized and reconstituted pure OF (LOF; 1%) and IVF without supplementation (control). Penetration, monospermy and IVF efficiency were evaluated. Transmission electron microscopy showed an EVs population primarily composed of exosomes (83%; 30-150 nm). Supplementation with poEVs during IVF increased monospermy compared with control (44% vs 17%) while maintaining an acceptable penetration rate (61% vs 78% respectively) in a similar way to OF and LOF. Western blotting revealed poEVs proteins involved in early reproductive events, including zona pellucida hardening. In conclusion, our finding show that poEVs are key components of porcine OF and may play roles in porcine fertilisation and polyspermy regulation, suggesting that supplementation with poEVs is a reliable strategy to decrease porcine polyspermy and improve in vitro embryo production outcomes
Synthesis and Biological Evaluation of PSMA Ligands with Aromatic Residues and Fluorescent Conjugates Based on Them
Prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), is a suitable target for specific delivery of antitumor drugs and diagnostic agents due to its overexpression in prostate cancer cells. In the current work, we describe the design, synthesis, and biological evaluation of novel low-molecular PSMA ligands and conjugates with fluorescent dyes FAM-5, SulfoCy5, and SulfoCy7. In vitro evaluation of synthesized PSMA ligands on the activity of PSMA shows that the addition of aromatic amino acids into a linker structure leads to a significant increase in inhibition. The conjugates of the most potent ligand with FAM-5 as well as SulfoCy5 demonstrated high affinities to PSMA-expressing tumor cells in vitro. In vivo biodistribution in 22Rv1 xenografts in Balb/c nude mice of PSMA-SulfoCy5 and PSMA-SulfoCy7 conjugates with a novel PSMA ligand demonstrated good visualization of PSMA-expressing tumors. Also, the conjugate PSMA-SulfoCy7 demonstrated the absence of any explicit toxicity up to 87.9 mg/kg. Β© 2021 American Chemical Society. All rights reserved