Glutathione de Novo Synthesis but Not Recycling Process Coordinates with Glutamine Catabolism to Control Redox Homeostasis and Directs Murine T Cell Differentiation

Upon antigen stimulation, T lymphocytes undergo dramatic changes in metabolism to fulfill the bioenergetic, biosynthetic and redox demands of proliferation and differentiation. Glutathione (GSH) plays an essential role in controlling redox balance and cell fate. While GSH can be recycled from Glutathione disulfide (GSSG), the inhibition of this recycling pathway does not impact GSH content and murine T cell fate. By contrast, the inhibition of the de novo synthesis of GSH, by deleting either the catalytic (Gclc) or the modifier (Gclm) subunit of glutamate–cysteine ligase (Gcl), dampens intracellular GSH, increases ROS, and impact T cell differentiation. Moreover, the inhibition of GSH de novo synthesis dampened the pathological progression of experimental autoimmune encephalomyelitis (EAE). We further reveal that glutamine provides essential precursors for GSH biosynthesis. Our findings suggest that glutamine catabolism fuels de novo synthesis of GSH and directs the lineage choice in T cells

Magnetic force driven magnetoelectric effect in bi-cantilever composites

The magnetic force driven magnetoelectric (ME) effect in bi-cantilever Mn-Zn-Ferrite /PZT composites is presented. Compared with single cantilever, the ME voltage coefficient in bi-cantilever composite is a little lower and the resonance frequency is higher, but the bi-cantilever structure is advantageous for integration. When the magnetic gap is 3 mm, the ME voltage coefficient can achieve 6.2 Vcm-1Oe-1 at resonance under optimum bias field Hm=1030 Oe; when the magnetic gap is 1.5 mm, the ME voltage coefficient can get the value as high as 4.4 Vcm-1Oe-1 under much lower bias field H=340 Oe. The stable ME effect in bi-cantilever composites has important potential application in the design of new type ME device

Equivalent circuit method for resonant magnetoelectric effect in disk-shaped laminated composites

A theoretical model based on equivalent circuit analytical method has been presented for the magnetoelectric (ME) effect in disk-shaped laminated composites. The derived expressions for the ME voltage coefficient can be used to describe the frequency response of ME effect under both open circuit and finite electrical resistance load conditions. It was shown that the resonance frequency and resonant ME voltage coefficient increase with the increase in electrical resistance load in disk-shaped ME laminated composite, which is consistent with the observations for plate-shaped one. The theoretical results show good agreement with the experimental results in Tb0.3Dy0.7Fe1.92 (Terfenol-D)/Pb(Zr,Ti)O3 (PZT)/Terfenol-D disk-shaped sandwich laminated composite. This work is significant for designing ME devices and their signal-processing and electronic circuits

Enhanced converse magnetoelectric effect in cylindrical piezoelectric-magnetostrictive composites

Enhanced converse magnetoelectric (ME) effect has been experimentally observed in cylindrical PZT-Terfenol-D piezoelectric-magnetostrictive bilayered composites, where the piezoelectric and magnetostrictive components are coupled through normal stresses instead of shear stresses that act in most of previous multiferroic composites. A theoretical model based on elastodynamics analysis has been proposed to describe the frequency response of converse ME effect for axial and radial modes in the bilayered cylindrical composites. The theory shows good agreement with the experimental results. The different variation tendency of resonant converse ME coefficient, as well as different variation rate of resonance frequency with bias magnetic field for axial and radial modes is interpreted in terms of demagnetizing effect. This work is of theoretical and technological significance for the application of converse ME effect as magnetic sensor, transducers, coil-free flux switch, etc

Theory of frequency response of magnetoelectric effects in radially polarized thin cylindrical composites

A theoretical model for the frequency response of magnetoelectric (ME) effect in thin cylindrical piezoelectric-magnetostrictive composites is presented by using constitutive and elastodynamic equations. The calculated results show that there is a resonant enhancement peak of ME voltage coefficient in the electromechanical resonance region and the ME voltage coefficient at resonance frequency exceeds that at low frequency by one or two orders of magnitude. The resonance frequency is predicted to increase with decreasing average diameter $\bar{D}$ and increasing thickness of magnetostrictive layer tM of the cylindrical composite, which is in good agreement with the experimental results reported in literatures. The corresponding resonance ME voltage coefficient increases with increasing tM , but reaches to a peak value and then decreases with increasing $\bar{D}$. It is indicated that the ME effect of trilayered cylindrical composite is less than that of bilayered one, possibly due to its symmetric structure. Under clamped condition, the resonance frequency will shift to a very high value for both trilayered and bilayered cylindrical composites, while the ME effect has different performance, enhanced in bilayered but suppressed in trilayered cylindrical composite. Our model shows that one can obtain strong ME effect and proper resonance frequency by selecting suitable materials, optimizing its geometry structure and varying mechanical boundary conditions for the cylindrical composite structur

Improved RANSAC Point Cloud Spherical Target Detection and Parameter Estimation Method Based on Principal Curvature Constraint

Spherical targets are widely used in coordinate unification of large-scale combined measurements. Through its central coordinates, scanned point cloud data from different locations can be converted into a unified coordinate reference system. However, point cloud sphere detection has the disadvantages of errors and slow detection time. For this reason, a novel method of spherical object detection and parameter estimation based on an improved random sample consensus (RANSAC) algorithm is proposed. The method is based on the RANSAC algorithm. Firstly, the principal curvature of point cloud data is calculated. Combined with the k-d nearest neighbor search algorithm, the principal curvature constraint of random sampling points is implemented to improve the quality of sample points selected by RANSAC and increase the detection speed. Secondly, the RANSAC method is combined with the total least squares method. The total least squares method is used to estimate the inner point set of spherical objects obtained by the RANSAC algorithm. The experimental results demonstrate that the method outperforms the conventional RANSAC algorithm in terms of accuracy and detection speed in estimating sphere parameters

Effect of Mold Opening Process on Microporous Structure and Properties of Microcellular Polylactide–Polylactide Nanocomposites

Cell structure is a key factor that determines the final properties of microcellular polylactide (PLA) product. In the mold opening process, adjusting the rate of mold opening can effectively control cell structure. PLA and PLA composites with a void fraction as high as 50% were fabricated using the mold opening technique. The effects of mold opening rate and the addition of nanoclay on the cell structure, mechanical properties, and surface quality of microcellular PLA and PLA composites samples were investigated. The results showed that finer cell structure was received in the microcellular PLA samples and the surface quality was improved effectively when decreasing the rate of mold opening. The effect of mold opening rate on the foaming behavior of microcellular PLA–nanoclay was the same as that of microcellular PLA. The addition of 5 wt % nanoclay significantly improved the foaming properties, such as cell density, cell size, and structural uniformity, which consequently enhanced the mechanical properties of foams and the surface quality

Development of a Combined Genetic Engineering Vaccine for Porcine Circovirus Type 2 and Mycoplasma Hyopneumoniae by a Baculovirus Expression System

Mycoplasma hyopneumoniae (Mhp) and porcine circovirus type 2 (PCV2) are the main pathogens for mycoplasmal pneumonia of swine (MPS) and post-weaning multisystemic wasting syndrome (PMWS), respectively. Infection by these pathogens often happens together and causes great economic losses. In this study, a kind of recombinant baculovirus that can display P97R1P46P42 chimeric protein of Mhp and the capsid (Cap) protein of PCV2 was developed, and the protein location was identified. Another recombinant baculovirus was constructed without tag proteins (EGFP, mCherry) and was used to evaluate the immune effect in experiments with BALB/c mice and domestic piglets. Antigen proteins P97R1P46P42 and Cap were expressed successfully; both were anchored on the plasma membrane of cells and the viral envelope. It should be emphasized that in piglet immunization, the recombinant baculovirus vaccine achieved similar immunological effects as the mixed commercial vaccine. Both the piglet and mouse experiments showed that the recombinant baculovirus was able to induce humoral and cellular responses effectively. The results of this study indicate that this recombinant baculovirus is a potential candidate for the further development of more effective combined genetic engineering vaccines against MPS and PMWS. This experiment also provides ideas for vaccine development for other concomitant diseases using the baculovirus expression system