Betaine and nano-emulsified vegetable oil supplementation for improving carcass and meat quality characteristics of broiler chickens under heat stress conditions

IntroductionThis research aimed to examine the effects of water-added betaine (BET) and/or nano-emulsified vegetable oil (MAGO) on carcass and meat quality characteristics of broilers raised under thermoneutral (TN) and heat stress (HS) conditions.MethodsOn day 21, 640 birds (Ross 308) were randomly assigned to one of two thermal conditions (thermoneutral 22 ± 1°C and heat stress 32 ± 1°C) each containing four treatment groups: Control, BET, MAGO, and a mixture of both (BETMAGO) in a 2 × 4 factorial arrangement (eight groups). Each group has eight replicates, with ten birds each. The birds' carcass and meat quality characteristics were evaluated at 35 days.Results and discussionThe dressing percentage, breast, leg, wing, heart, initial pH, color change, cooking loss (CL), water-holding capacity (WHC), shear force (SF), and texture profile with exception of springiness significantly affected by the treatments. The results showed that HS had negative effects on carcass weight and relative weights of the breast, spleen, and heart. Moreover, HS increased dressing percentage, wing, initial pH, final core temperature, initial lightness, WHC, and hardness. Significant differences in interactions between treatments and temperature were observed in the spleen, WHC, and SF.ConclusionWater supplemented with BET effectively improved carcass dressing percentage, breast weight, and meat quality in terms of water-holding capacity and tenderness under HS conditions. More studies on the use of BET and/or MAGO at different levels were recommended

Feed supplementation with some natural products on Salmonella infected broilers’ performance and intestinal injury during the starter period

Many alternatives are under investigation since 2006 when the European Union banned the use of antimicrobials in animal feed. The objective of this study was to find the effect of different feed additives in comparison with antibiotic on the performance and gut health in broiler infected with Salmonella during the starter phase. A total of 240 one day old male Ross 308 chickens were divided into six groups as follow: control treatment (negative control), orally challenged with Salmonella enterica subsp. Typhimurium (positive control), T1: oral challenge + 0.1 g/kg antibiotic, T2: oral challenge + 0.15 g/kg Sanguinarine, T3: oral challenge + 1 g/kg oregano powder, T4: oral challenge + calcium montmorillonite. On day second of the experiment, challenge was performed at a dose rate of 1.0 mL of the inoculum (4.5 × 108 CFU/mL). Data were statistically analysed with the help of Analysis of Variance (ANOVA) for repeated measures using least significant difference (LSD) as post-hoc test. Feed consumption was significantly (p  .05) between the control and experimental groups. Significantly (p < .01) higher villus width was found in T1, T4 and negative control. Similarly, villus height was higher (p < .05) in T2 and T4 compared to the control. It was concluded from the present study that different natural feed additives produced promising results in term of improved growth traits and gut health in Salmonella infected broilers. However, Sangrovit Extra produced better results in comparison with other treatments.HIGHLIGHTS Body weight, feed efficiency were improved in Sangrovit Extra fed broiler. Production efficiency factor was enhanced in Sangrovit Extra fed broiler. Villus height was significantly higher in Sangrovit Extra fed broiler. Villus width was enhanced in antibiotic treated broiler

Effects of the Sous Vide and Conventional Electric Oven Cooking Methods on the Physio-Sensory Quality Attributes of Arabian Camel (<i>Camelus dromedarius</i>) Meat

This study aimed to evaluate the effect of the sous vide and electric oven cooking methods on the physical and sensory characteristics of camel meat. A combination of 4 cooking temperatures (70, 80, 90, and 100 °C) and 6 cooking times (30, 60, 90, 120, 150, and 180 min) was applied. Both methods significantly affected the meat’s physical properties (pH, cooking loss, density, lightness, redness, and yellowness color components), except for water activity. Furthermore, the cooking temperature and time significantly affected all the sensory properties (tenderness, flavor, juiciness, and general acceptance). The cooking loss was the only parameter affected significantly by the interaction of the cooking method, cooking temperature, and cooking time. It is concluded that the sous vide method is the more suitable method for cooking camel meat compared to the electric oven method considering the cooking temperature and time. Further studies are recommended to estimate energy consumption for both cooking methods evaluated in this study aiming at reducing the overall power expenditure

Different levels of single-strain probiotic (Bacillus subtilis) with proteolytic enzyme (serratiopeptidase) can be used as an alternative to antibiotic growth promoters in broiler

ABSTRACT: In the current study, the proteolytic enzyme (serratiopeptidase) was used to enhance the efficacy of Bacillus subtilis (B. subtilis) probiotic as a growth promotor in broiler chicken. The effects of serratiopeptidase on the efficacy of different levels of B. subtilis as a growth promotor in broiler chicks were evaluated regarding growth performance traits, villus histomorphometric characterization, and intestinal microbiota count. Day-old broiler chicks (n = 120) were allocated into 4 groups having 3 replicates/group. In the control group (C), the basal diet was kept without supplementation. In treatment groups (P100, P150, and P200), the basal diet was supplemented with 100, 150, and 200 mg probiotics, respectively besides 30 mg proteolytic enzyme in the 3 treated groups for 4 wk. The performance parameters were significantly affected by the supplementation of serratiopeptidase to the B. subtilis treatment groups. Feed intake (FI), body weight gain (WG), feed conversion ratio (FCR), and dressing percent were significantly improved in the treatment groups as compared to the control group. Significantly, the lowest feed intake was recorded for the P200 group. The highest body weight gain and dressing percentage were recorded for the P200 group. An improved FCR was recorded in the P200 group (1.7) as compared to the control group. The different levels of B. subtilis supplemented with serratiopeptidase revealed significant improvements (P<0.05) in the morphology of the intestine by showing increases in villus height and width and crypt depth of the small intestine. The microbial count revealed that E. coli and salmonella colonies were significantly reduced in the P200 group as compared to the control and other treatment groups. In conclusion, the supplementation of B. subtilis with serratiopeptidase as a growth promoter in broiler chicks significantly improved the overall performance, and intestinal health and reduced microbial load contributing to optimizing the performance of broiler chickens. The greatest improvement was observed in the P200 group fed with B. subtilis as a probiotic and serratiopeptidase enzyme (200 mg:30 mg)

Ameliorative Effects of Antibiotic-, Probiotic- and Phytobiotic-Supplemented Diets on the Performance, Intestinal Health, Carcass Traits, and Meat Quality of Clostridium perfringens-Infected Broilers

The poultry industry needs efficient antibiotic alternatives to prevent necrotic enteritis (NE) infections. Here, we evaluate the effects of probiotic and/or prebiotic dietary supplementation on performance, meat quality and carcass traits, using only an NE coinfection model, in broiler chickens. Three hundred and twenty-four healthy Ross 308 broiler chicks are allocated into six groups. Taking a 35 d feeding trial, the chicks are fed a basal diet with 0.0, 0.1, 0.5, 0.12, 0.5 + 0.12, and 0.2 g Kg&minus;1 for the control (T1), Avilamycin (Maxus; T2), live probiotic (CloStat (Bacillus subtilis);T3), natural phytobiotic compounds (Sangrovit Extra (sanguinarine and protopine); T4), CloStat + Sangrovit Extra (T5), and spore probiotic strain (Gallipro Tect (Bacillus subtilis spores); T6) treatments, respectively. Occurring at 15 days-old, chicks are inoculated with Clostridium perfringens. The obtained results reveal that all feed additives improve the performance, feed efficiency, and survival rate, and reduces the intestinal lesions score compared with the control group. The T6 followed by T3 groups show a significant (p &lt; 0.05) increase in some carcass traits, such as dressing, spleen, and thymus percentages compared with other treatments. Also, T5 and T6 have significantly recorded the lowest temperature and pHu values and the highest hardness and chewiness texture values compared to the other treated groups. To conclude, probiotics combined with prebiotic supplementation improves the growth, meat quality, carcass characterization and survival rate of NE-infected broiler chickens by modulating gut health conditions and decreasing lesion scores. Moreover, it could be useful as an ameliorated NE disease alternative to antibiotics in C. perfringens coinfected poultry

Chemical Composition and Quality Characteristics of Meat in Three One-Humped Camel (Camelus dromedarius) Breeds as Affected by Muscle Type and Post-Mortem Storage Period

The influence of muscle type and postmortem storage period on meat chemical composition and quality attributes of three breeds of camels (Baladi Saudi, Pakistani, and Somali) were investigated in this study. Crude fat and ash content were significantly higher in the Pakistani than in the Baladi Saudi and Somali breeds, except for higher moisture content observed in the Somali breed. The longissimus lumborum (LL) and semimembranosus (SM) muscles had a greater crude protein than the biceps femoris (BF) muscle. Storage period exhibited a significant reduction in pH values and improvement in color components of meat. The Somali breed produced higher cooking loss % and shear force, with a lower water holding capacity than the Baladi Saudi and Pakistani breeds. The LL muscle had better cooking loss %, water holding capacity, and shear force, whereas storage period (7 days) exhibited a significant reduction in the myofibrillar fragmentation index. Baladi Saudi and Pakistani breeds and LL muscle samples presented better meat sensory attributes, while storage period had no significant influence on the overall sensory characters of meat. In conclusion, there were significant differences between the chemical and structural characteristics of the LL, BF, and SM muscle samples among the three breeds of camel. Baladi Saudi and Pakistani had better meat quality traits than the Somali breed. In addition, LL muscles had better nutritional values and meat quality parameters than BF and SM muscles. Improvement in meat quality attributes were achieved with the storage process of 7 days. It is observed that, the Saudi Baladi camels have a merit of low fat content over both Somali and Pakistani camel breeds. It is also concluded that no significant effects were observed between the treatments as a result of storage when sensory attributes were considered. Moreover, breed, muscle and storage period were interacted significantly only with regard to lightness color space and shear force. This is useful knowledge for the meat industry for optimizing processing and storage procedures for various camel muscles

Effect of Rumex nervosus Leaf Powder on the Breast Meat Quality, Carcass Traits, and Performance Indices of Eimeria tenella Oocyst-Infected Broiler Chickens

This work aimed to assess the effect of using the RNL on performance indices, carcass trait, and meat quality of broiler chicken infected with Eimeria tenella compared with commercially used anticoccidials salinomycin. Moreover, we compare these selected variables between infected medicated groups and a non-infected unmedicated group (UUT) and an infected unmedicated group (IUT). A total of 150 1-day-old Ross 308 mixed-sex broilers were divided into 6 groups. Birds in groups 1, 2, and 3 were fed basic diets supplemented with 1, 3, and 5 g of RNL/kg diet, respectively. Group 4 received a basic diet with 66 mg of salinomycin. The control groups (5 and 6) were given a basic diet with no RNL or salinomycin added. All groups except the negative controls were challenged with Eimeria tenella at 21 days old. Birds in RNL groups outperformed those in the IUT group in performance indices, and they have a similar effect to the salinomycin group. Enhancement-infected birds with RNL affected some chickens’ carcass traits. Drip loss, water-holding capacity, and meat tenderness were improved by RNL inclusion (1 g) in the diet. In conclusion, the meat of infected birds receiving 1 g RNL&nbsp;had increased quality attributes, with preferable tenderness and springiness when compared to the IUT group. RNL could therefore also be considered a promising non-conventional feed source in the future. Further research is needed to optimize the use of RNL to improve broilers production and meat quality in both infected and non-infected conditions

Responses of egg quality sustainability, sensory attributes and lipid profile of eggs and blood to different dietary oil supplementations and storage conditions

The aim of this study is to investigate the responses of egg quality, sensory attributes and lipid profile of eggs and blood serum to different combinations of dietary oil supplementations and storage conditions. Thus, laying hens aged 25 weeks were assigned to six experimental groups and fed different diets for 14 weeks. The group fed an unsupplemented diet served as control, while the other five treatment groups consumed the same basal diet supplemented with 5% corn oil, 5% linseed oil, 2% corn oil + 3% linseed oil, 3% corn oil + 2% fish oil and 3% linseed oil + 2% fish oil, respectively. After 10 weeks, 36 eggs were collected from each experimental group and divided into three sets of 12 eggs to compare the quality of fresh eggs with those obtained after 21 days of storage at 23 °C and 5 °C. In addition, 20 untrained panellists evaluated the sensory characteristics of fresh eggs. Except for shell thickness, shell percentage and shell weight per unit of surface area (SWUSA), the supplementation of oil did not affect the egg quality parameters. The eggs laid by hens fed 3% corn oil and 2% fish oil had the highest quality shells, while the eggs from the control group had the best albumen colour, flavour and general acceptance. However, only shell percentage and shape index were unaffected by storage conditions, while all other parameters were significantly lower in eggs stored at 23 °C for 21 days. Although dietary oil supplementation did not significantly improve egg quality, feeding 3% corn oil and 2% fish oil improved eggshell quality, which declined with increasing storage temperature/period. HIGHLIGHTS Dietary oils are essential components of poultry diets and animal nutrition. Sensory attributes are essential for human acceptance of animal products. Lipid profile may reflect lipid utilisation and efficiency. Different dietary oil combinations affect the eggshell quality and sensory attributes of eggs. Linseed oil enhanced the nutritional value of egg lipids and serum lipid profiles

The effects of betaine and nano-emulsified plant-oil supplementation on growth performance and serum biochemistry indices of heat-stressed broiler chickens

This study aimed to investigate the effects of water-added betaine (BET) and Magic oil, (MAGO) on growth performance and blood biochemistry of broilers exposed to constant heat stress (32 ± 1 °C) during 21–35 d. On day 21, 320 chicks (Ross 308) were straight run to one of four groups based supplements with drinking water (DW): control, BET (1 g/L DW), and/or MAGO (1 ml/L DW), respectively). Each treatment consisted of eight replicates with ten birds each. The performance data were obtained at 21, 28, and 35 d. Serum biochemical data of the birds were estimated at day 35. The results showed that body weight gain (BWG) and feed intake did not differ significantly (p > .05) between the treatments. However, Bet alone or in combination with MAGO has improved feed conversion ratio (FCR) during the 28–35 d and 21–35 d periods; also, BET lowered (p < .05) creatinine compared to control. The addition of MAGO alone or in combination with BET increased (p < .05) total protein, albumin, globulin, and low-density lipoprotein-cholesterol. In addition, MAGO decreased total lipids, and creatinine, whereas it increased (p < .05) cholesterol, especially good cholesterol, and triglycerides compared with the control. In conclusion, water-BET-supplements alone or in combination with MAGO has improved the FCR under heat stress conditions. Therefore, it is recommended to use Bet alone or both supplements under high-temperature conditions in broiler houses. However, further studies on the use of MAGO and/or BET at different doses are needed to maximise BWG and maintain the productivity of the birds.Highlights Water-enriched magic oil and betaine had higher added value than feeding alone by improving the feed conversion ratio during 21–35 d. Compared with the other groups, water-supplemented magic oil produced some benefits, such as lower serum glucose, higher serum high-density lipoprotein ‘good cholesterol’ and lower creatinine levels. The added value of the combination of magic oil and betaine for growth performance and serum health biomarkers needs further investigation at various levels