4 research outputs found
Comparative Study of Substrate- and Stereospecificity of Penicillin G Amidases from Different Sources and Hybrid Isoenzymes
Original enzyme-catalyzed synthesis of chalcones: Utilization of hydrolase promiscuity
E-chalcone was obtained with very high stereoselectivity for the first time
by an enzyme-catalyzed Claisen-Schmidt condensation between benzaldehyde and
acetophenone. From a set of lipases, only that from hog pancreas demonstrated
promiscuity, catalyzing the reaction in the presence of imidazole as a
promoter. Another enzyme, acylase from Aspergillus melleus (3.5.1.14) also
proved to be active in the synthesis of E-chalcone under the same reaction
conditions. This acylase along with the recombinant D-aminoacylase (3.5.1.81)
catalyzed also the reaction between acetophenone and p-nitrobenzaldehyde.
Such “green” approach to synthesis of chalcones is of great interest, because
of their important applications as formula ingredients in pharmaceutical,
food and cosmetic industry