9 research outputs found
Anemonia sulcata and Its Symbiont Symbiodinium as a Source of Anti-Tumor and Anti-Oxoxidant Compounds for Colon Cancer Therapy: A Preliminary in Vitro Study
Simple Summary: Colorectal cancer is one of the most frequent types of cancer in the population.
Recently, invertebrate marine animals have been investigated for the presence of natural products
which can damage tumor cells, prevent their spread to other tissues or avoid cancer develop. We
analyzed the anemone Anemonia sulcata with and without the presence of its microalgal symbiont
(Symbiodinium) as a source of bioactive molecules for the colorectal cancer therapy and prevention.
Colon cancer tumor cells were exposed to Anemone extracts observing a remarkable cell death and
a great antioxidant capacity. These preliminary results support that Anemonia sulcata could be a
source of bioactive compounds against colorectal cancer and that the absence of its symbiont may
enhance these properties. Further studies will be necessary to define the bioactive compounds of
Anemonia sulcata and their mechanisms of action.
Abstract: Recently, invertebrate marine species have been investigated for the presence of natural
products with antitumor activity. We analyzed the invertebrate Anemonia sulcata with (W) and without
(W/O) the presence of its microalgal symbiont Symbiodinium as a source of bioactive compounds
that may be applied in the therapy and/or prevention of colorectal cancer (CRC). Animals were
mechanically homogenized and subjected to ethanolic extraction. The proximate composition and
fatty acid profile were determined. In addition, an in vitro digestion was performed to study the
potentially dialyzable fraction. The antioxidant and antitumor activity of the samples and the digestion
products were analyzed in CRC cells in vitro. Our results show a high concentration of
polyunsaturated fatty acid in the anemone and a great antioxidant capacity, which demonstrated
the ability to prevent cell death and a high antitumor activity of the crude homogenates against
CRC cells and multicellular tumor spheroids, especially W/O symbiont. These preliminary results
support that Anemonia sulcata could be a source of bioactive compounds with antioxidant and antitumor
potential against CRC and that the absence of its symbiont may enhance these properties.
Further studies will be necessary to define the bioactive compounds of Anemonia sulcata and their
mechanisms of action.International Excellence Campus of the Sea (CEI.MAR)
CEIJ-007
CEIJ-001
Bioavailability and biotransformation of linolenic acid from basil seed oil as a novel source of omega-3 fatty acids tested on a rat experimental model
Basil is an aromatic herb with a high concentration of bioactive compounds. The oil extracted from its
seeds is a good source of α-linolenic acid (ALA) and also provides substantial amounts of linoleic acid
(LA). This study aimed to test the bioavailability of the oil derived from basil seeds and its effects on
different physiological parameters using 7–15% dietary inclusion levels. Furthermore, the assimilation of
LA and ALA and their transformation in long-chain polyunsaturated fatty acids (LC-PUFAs) have been
studied. Digestive utilization of total fat from basil seed oil (BSO) was high and similar to that of olive oil
used as a control. Consumption of BSO resulted in increased LA and ALA levels of the plasma, liver, and
erythrocyte membrane. In addition, the transformation of LA to arachidonic acid (ARA) was decreased by
the high dietary intake of ALA which redirected the pathway of the Δ-6 desaturase enzyme towards the
transformation of ALA into eicosapentaenoic acid (EPA). No alterations of hematological and plasma biochemical
parameters were found for the 7 and 10% dietary inclusion levels of BSO, whereas a decrease in
the platelet count and an increase in total- and HDL-cholesterol as well as plasma alkaline phosphatase
(ALP) were found for a 15% BSO dose. In conclusion, BSO is a good source of ALA to be transformed into
EPA and decrease the precursor of the pro-inflammatory molecule ARA. This effect on the levels of EPA
in different tissues offers potential for its use as a dietary supplement, novel functional food, or a constituent
of nutraceutical formulations to treat different pathologies.Spanish GovernmentEuropean Commission PTQ-17-09172
RTC-2017-6540-1
RTI2018-100934-B-I00
RTC2019-006870-1Andalusian Government AGR145
CTS164
CTS-10
Novel insights and mechanisms of diet-induced obesity: Mid-term versus long-term effects on hepatic transcriptome and antioxidant capacity in Sprague-Dawley rats
Supplementary data to this article can be found online at https://doi.org/10.1016/j.lfs.2023.121746Aims
The study of molecular mechanisms related to obesity and associated pathologies like type 2-diabetes and non-alcoholic fatty liver disease requires animal experimental models in which the type of obesogenic diet and length of the experimental period to induce obesity deeply affect the metabolic alterations. Therefore, this study aimed to test the influence of aging along a rat model of diet-induced obesity in gene expression of the hepatic transcriptome.
Main methods
A high-fat/high-fructose diet to induce obesity was used. Mid- (13 weeks) and long-term (21 weeks) periods were established. Caloric intake, bodyweight, hepatic fat, fatty acid profile, histological changes, antioxidant activity, and complete transcriptome were analyzed.
Key findings
Excess bodyweight, hepatic steatosis and altered lipid histology, modifications in liver antioxidant activity, and dysregulated expression of transcripts related to cell structure, glucose & lipid metabolism, antioxidant & detoxifying capacity were found. Modifications in obese and control rats were accounted for by the different lengths of the experimental period studied.
Significance
Main mechanisms of hepatic fat accumulation were de novo lipogenesis or altered fatty acid catabolism for mid- or long-term study, respectively. Therefore, the choice of obesity-induction length is a key factor in the model of obesity used as a control for each specific experimental design.Spanish Ministry of Science and Innovation, and the European Union
through projects B-AGR-662-UGR20, RTI-2018-100934-B-I00, and the
FEDER program, respectivel
Antitumor Effect of the Ethanolic Extract from Seeds of Euphorbia lathyris in Colorectal Cancer
The seeds of Euphorbia lathyris have been used in traditional medicine to treat various
medical conditions. However, neither all of their active biocompounds nor the molecular mechanisms
underlying their therapeutic effects have been described. A new ethanolic extract of defatted flour
from mature seeds of Euphorbia lathyris showed a high total polyphenol content and significant
antioxidant activity. Chromatographic analysis showed that esculetin, euphorbetin, gaultherin, and
kaempferol-3-rutinoside were the most abundant polyphenolic bioactive compounds. Antiproliferative assays showed a high and selective antitumor activity against colon cancer cell lines (T84
and HCT-15). In addition, a significant antiproliferative activity against glioblastoma multiforme
cells was also demonstrated. Its mechanism of action to induce cell death was mediated by the
overexpression of caspases 9, 3, and 8, and by activation of autophagy. Interestingly, a reduction
in the migration capacity of colon cancer cells and a significant antiangiogenic effect on human
umbilical vein endothelial cells were also demonstrated. Finally, the extract significantly reduced the
subpopulations of cancer stem cells. This extract could be the basis to develop new therapeutic strategies for the treatment of colon cancer, although further experiments will be necessary to determine
its in vivo effects.Granada University (project PSE/17/002), Ministerio de
Ciencia e Innovación (project RTC-2017-6540-1) and by the CTS-107 and AGR145 Groups
MOOC como herramienta para el aprendizaje de contenidos específicos en el área de la Fisiología
Teniendo en cuenta que el aprendizaje integral y la implementación de las TICs están cada vez más integradas en el sistema educativo, la creación de “cápsulas educativas”-MOOCs son una herramienta creativa que puede ayudar al alumnado en la asimilación de los conocimientos. Además, al ser una herramienta de duración corta les obliga resumir la información, destacar la más importante y ser capaces de trasmitirla. Para ello, el objetivo principal de esta propuesta de innovación docente ha sido elaborar una nueva herramienta docente y aplicarla en el área de Fisiología, en concreto en la asignatura de Fisiología Celular y Humana del Grado en Farmacia. Para ello se formaron diferentes grupos de estudiantes a los que les asignó una temática relacionada con el área de Fisiología y dos tutores (participantes en el equipo de investigación) que los han acompañado en la creación de MOOC. Por otro lado, se creó el grupo de evaluadores externos, responsables para la evaluación de la capsula educativa definitiva. Para facilitar el desarrollo del proyecto, antes del comienzo del proyecto se elaboraron y se consensuaron las rúbricas necesarias para evaluar el proceso de la creación de MOOC por parte de los tutores y evaluadores. La comunicación entre los grupos de estudiantes y de los miembros del equipo de investigación se ha realizado usando el correo electrónico y con documentos compartidos en la nube de la UGR. Las diferentes tutorías entre los tutores asignados y los grupos de los alumnos, se han realizado por Google Meet. La nota final de las capsulas educativas, ha sido resultado de la interacción tanto del equipo de tutores como de evaluadores externos. Al final del proceso se ha hecho una exposición de las capsulas educativas en la clase con los demás compañeros. Al finalizar el proceso, se ha utilizado la plataforma de Google Forms para recibir las opiniones de los estudiantes y recoger sus propuestas. Según las opiniones manifestadas, la iniciativa de aplicar esta herramienta en el área de Fisiología ha recibido muy buenas críticas sobre todo sobre la innovación de la propuesta. El alumnado por otro lado destacó que le gustaría encontrar esta herramienta en otras asignaturas. Sin embargo, también mencionaron que les gustaría saber algo más sobre aplicaciones disponibles para elaborar los videos y poder ser aún más creativos. Por último, se usará la plataforma zenodo para la difusión y divulgación de los trabajos creados para que los demás estudiantes puedan consultarlos. Los resultados de esta propuesta se publicarán en congresos docentes nacionales e internacionales con el objetivo de compartir la experiencia con otros docentes e intentar implementar esta herramienta en asignaturas de áreas afines.Considering that comprehensive learning and the implementation of ICTs are increasingly integrated into the educational system, the creation of "educational capsules"-MOOCs are a creative tool that can help students assimilate knowledge. In addition, having a limited duration forces them to summarize the information, highlight the most important and be able to transmit it. For this, the main objective of this teaching proposal was the development of a new teaching tool and apply it in Physiology, specifically in the subject of Cellular and Human Physiology of the Degree in Pharmacy. For this, different groups of students were formed and a specific subject was assigned to them, related to the area of Physiology. Moreover, two tutors (member of the research team) were assigned to each group and have accompanied them in the creation of MOOCs. On the other hand, the group of external evaluators, responsible for the evaluation of the educational capsule, was created. To facilitate the development of the project, prior to the beginning of the project, the necessary rubrics were prepared and discussed between the different members, to be able to evaluate the process of creating MOOCs by both the tutors and evaluators. Communication between the student groups and the members of the research team was maintained using emails and documents were shared in the UGR cloud. The meetings between the assigned tutors and the student groups have been carried out through Google Meet. The grade of the educational capsules has resulted as the median of tutors as well the external evaluators. Once created, MOOCs were presented in the rest of the classroom with the other classmates. At the end of the process, the Google Forms platform has been used to fulfil a questionnaire and collect the opinions of the students and collect their proposals for future improvements. According to the opinions expressed, the initiative to apply this tool in the Physiology area has received very good reviews by the students, especially regarding the innovation of the proposal. The students, on the other hand, highlighted that they would like to be able to use this tool in other subjects of their carreer. However, they also mentioned that they would like to know more about available applications to make videos, a fact that would help them be even more creative. Finally, the zenodo platform will be used for the dissemination of the works created so that other students can consult them. The results of this proposal will be published in national and international teaching conferences with the aim of sharing the experience with other teachers and trying to implement this tool in subjects in related areas.Universidad de Granad
Extracto etanólico de semillas de Citrullus colocynthis, método para obtenerlo, composición farmacéutica que lo contiene y su uso como agente antitumoral
Número de publicación: 2906476. Número de solicitud: 202031035.La presente invención proporciona un método para la
obtención de un extracto etanólico de semillas
maduras de Citrullus colocynthis, caracterizado por
extracción con una solución hidroalcohólica con
etanol al 50%, a pH ácido, bajo atmósfera de
nitrógeno y durante tiempos cortos, no superiores a 1
- 2 h . Los extractos obtenidos de diferentes
variedades de Citrullus colocynthis poseen alto
contenido en polifenoles y muestran una potente
actividad antitumoral frente a líneas celulares de
adenocarcinoma de colon y de glioblastoma, incluso
frente a líneas celulares derivadas de tumores
resistentes a quimioterapia, todo ello con baja
toxicidad para hepatocitos humanos; también
presentan actividad frente a líneas células de
adenocarcinoma de páncreas. Por ello se propone el
extracto de la invención para uso en el tratamiento de
cáncer colorrectal , cáncer de páncreas y
glioblastoma.Universidad de Granad
Extracto etanólico de semillas de Euphorbia lathyris, método para obtenerlo, composición farmacéutica que lo contiene y su uso como agente antitumoral
Número de publicación: 2879098. Número de solicitud: 202030454.La presente invención proporciona un método para la
obtención de un extracto etanólico de semillas
maduras de Euphorbia lafhyris, preferiblemente
desengrasadas, caracterizado por el uso de una
solución de extracción con etanol al 50%, a pH ácido,
preferiblemente bajo atmósfera de nitrógeno y durante
tiempos cortos, no superiores a 1 - 2 h. Los
extractos obtenidos son ricos en compuestos
fenólicos, entre ellos compuestos fenólicos con
actividad anticancerígena. Dichos extractos muestran
una potente actividad antitumoral frente a líneas
celulares de adenocarcinoma de colon y de páncreas
y glioblastoma, que no se había detectado antes para
extractos de semilla de Euphorbia lafhyris, con baja
toxicidad para células sanas, Por ello, se propone
estos extractos, especialmente los obtenidos de
semillas desengrasadas, para uso en el tratamiento
de cáncer colorrectal, cáncer de páncreas y
glioblastoma.Universidad de Granad
Extracto etanólico de semillas de Solanum melongena, método para obtenerlo, composición farmacéutica que lo contiene y su uso como agente antitumoral
Número de publicación: 2906473. Número de solicitud: 202031034.La presente invención proporciona un método para la
obtención de un extracto etanólico de semillas
maduras de Solanum melongena, preferiblemente
desengrasadas, caracterizado por extracción con una
solución hidroalcohólica con etanol al 50%, a pH
ácido, preferiblemente bajo atmósfera de nitrógeno y
durante tiempos cortos, no superiores a 1 - 2 h. Los
extractos obtenidos de diferentes variedades de
Solanum melongena poseen alto contenido fenólico y
muestran una potente actividad antitumoral frente a
líneas celulares de adenocarcinoma de colon,
adenocarci noma de páncreas y glioblastoma
multiforme, incluso frente a líneas resistente a
quimio terapia, pero con baja toxicidad para
hepatocitos humanos. Por ello, se proponen los
extractos de la invención para uso en el tratamiento
de adenocarcinoma de colon, adenocarcinoma de
páncreas y glioblastoma multiforme, incluso
resistentes a las quimioterapias actualmente
utilizadasUniversidad de Granad
In Vivo Nutritional Assessment of the Microalga Nannochloropsis gaditana and Evaluation of the Antioxidant and Antiproliferative Capacity of Its Functional Extracts
This research was funded by the International Excellence Campus of the Sea (CEI-MAR) through Project CEIJ-016, as well as the Spanish Ministry of Science, Innovation and Universities and the European Union through projects RTC-2017-6540-1 and RTI-2018-100934-B-I00 and the FEDER program, respectively. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.Nannochloropsis gaditana is a microalga with interesting nutritional and functional value
due to its high content of protein, polyunsaturated fatty acids, and bioactive compounds. However,
the hardness of its cell wall prevents accessibility to these components. This work aimed to study
the effect of a treatment to increase the fragility of the cell wall on the bioavailability of its nutrients
and functional compounds. The antioxidant and antiproliferative capacity of functional extracts
from treated and untreated N. gaditana was assessed, and the profile of bioactive compounds was
characterized. Furthermore, to study the effect of treatment on its nutrient availability and functional
capacity, an in vivo experiment was carried out using a rat experimental model and a 20% dietary
inclusion level of microalgae. Functional extracts from treated N. gaditana exhibited higher antioxidant
activity than the untreated control. Furthermore, the treated microalga induced hypoglycemic action,
higher nitrogen digestibility, and increased hepatic antioxidant activity. In conclusion, N. gaditana
has interesting hepatoprotective, antioxidant, and anti-inflammatory potential, thus proving itself an
ideal functional food candidate, especially if the microalga is treated to increase the fragility of its cell
wall before consumption.International Excellence Campus of the Sea (CEI-MAR) CEIJ-016Spanish Government
European Commission RTC-2017-6540-1
RTI-2018-100934-B-I0