Efecto de dos reguladores de crecimiento y condiciones de iluminación en la germinación de semillas conservadas de Clitoria ternatea

The seeds viability lost in the seed legume bank of Research Institute of Pastures and Forages (IIPF) led to the aim of the work it was to determine the effect of two plant growth regulators (gibberellic acid; GA3 and naphthalene acetic acid; ANA) and illumination conditions on the germination of Clitoria ternatea SC-136 conserved seeds. One experiment was performed with two-factor completely randomized design with four replications Five different levels of growth regulators (factor A) and two illumination conditions (factor B) were evaluated. The variables measured were: total germination percentage (PTG) and angular transformation, days to 50% PTG (G50) and the days between 10 and 90% PTG (G10-90). In addition, morphological variables were evaluated. It was found that the addition of plant growth regulators (GA3 and NAA) was effective in increasing germination of Clitoria ternatea SC-136 conserved seeds, but not the illumination conditions tested. Combination GA3 (1 mg l-1) and NAA (0.1 mg l-1) to stimulate germination was recommended. Key words: GA3, germplasm, legume, NAA, photoperiod.Pérdidas en la viabilidad de semillas en el banco de germoplasma de leguminosas forrajeras del Instituto de Investigaciones de Pastos y Forrajes (IIPF) motivaron el objetivo del trabajo que fue determinar el efecto de dos reguladores de crecimiento vegetal (ácido giberélico; AG3 y ácido naftalenacético; ANA) y las condiciones de iluminación sobre la germinación de semillas conservadas de Clitoria ternatea SC-136. Se realizó un experimento bifactorial con diseño completamente al azar y cuatro repeticiones donde se evaluaron cinco niveles diferentes de reguladores de crecimiento (factor A) y dos condiciones de iluminación (factor B). Las variables medidas fueron: porcentaje total de germinación (PTG) y su transformación angular, los días hasta el 50% del PTG (G50) y los días entre el 10 y 90% del PTG (G10-90). Además, se evaluaron variables morfológicas. Se comprobó que la adición de reguladores de crecimiento vegetal (AG3 y ANA) tuvo efecto en el incremento de la germinación de semillas conservadas de Clitoria ternatea SC-136, no así las condiciones de iluminación probadas. Se recomendó la combinación AG3 (1 mg l-1) y ANA (0.1 mg l-1) para estimular su germinación. Palabras clave: AG3, ANA, fotoperíodo, germoplasma, leguminosa

Discriminant and Concurrent Validity of a Simplified DSM-Based Structured Diagnostic Instrument for the Assessment of Autism Spectrum Disorders in Youth and Young Adults

Background: To evaluate the concurrent and discriminant validity of a brief DSM-based structured diagnostic interview for referred individuals with autism spectrum disorders (ASDs). Methods: To test concurrent validity, we assessed the structured interview's agreement in 123 youth with the expert clinician assessment and the Social Responsiveness Scale (SRS). Discriminant validity was examined using 1563 clinic-referred youth. Results: The structured diagnostic interview and SRS were highly sensitive indicators of the expert clinician assessment. Equally strong was the agreement between the structured interview and SRS. We found evidence for high specificity for the structured interview. Conclusions: A simplified DSM-based ASD structured diagnostic interview could serve as a useful diagnostic aid in the assessment of subjects with ASDs in clinical and research settings

Effect of two plant growth regulators and illumination conditions in the germination of conserved seeds of<i> Clitoria ternatea</i>

The seeds viability lost in the seed legume bank of Research Institute of Pastures and Forages (IIPF) led to the aim of the work it was to determine the effect of two plant growth regulators (gibberellic acid; GA3 and naphthalene acetic acid; ANA) and illumination conditions on the germination of Clitoria ternatea SC-136 conserved seeds. One experiment was performed with two-factor completely randomized design with four replications Five different levels of growth regulators (factor A) and two illumination conditions (factor B) were evaluated. The variables measured were: total germination percentage (PTG) and angular transformation, days to 50% PTG (G50) and the days between 10 and 90% PTG (G10-90). In addition, morphological variables were evaluated. It was found that the addition of plant growth regulators (GA3 and NAA) was effective in increasing germination of Clitoria ternatea SC-136 conserved seeds, but not the illumination conditions tested. Combination GA3 (1 mg l-1) and NAA (0.1 mg l-1) to stimulate germination was recommended. Key words: GA3, germplasm, legume, NAA, photoperiod

Discriminant and concurrent validity of a simplified DSM-based structured diagnostic instrument for the assessment of autism spectrum disorders in youth and young adults

Abstract Background To evaluate the concurrent and discriminant validity of a brief DSM-based structured diagnostic interview for referred individuals with autism spectrum disorders (ASDs). Methods To test concurrent validity, we assessed the structured interview's agreement in 123 youth with the expert clinician assessment and the Social Responsiveness Scale (SRS). Discriminant validity was examined using 1563 clinic-referred youth. Results The structured diagnostic interview and SRS were highly sensitive indicators of the expert clinician assessment. Equally strong was the agreement between the structured interview and SRS. We found evidence for high specificity for the structured interview. Conclusions A simplified DSM-based ASD structured diagnostic interview could serve as a useful diagnostic aid in the assessment of subjects with ASDs in clinical and research settings.</p