1,877 research outputs found
Intravenous Penicillin for Antenatal Syphilotherapy
A 21 year old woman (G2 P0101)
of 24 weeks gestation presented with syphilis of unknown duration.
Sonography revealed fetal hydrops and placental thickening. Weekly intramuscular injections of 2.4
million U Bicillin for 3 weeks was initiated as recommended by the Centers for Disease Control.
Repeat sonogram 1 week after starting treatment revealed increased ascites and a new pericardial
effusion. Due to the worsening fetal condition, therapy was altered and the patient was admitted for
IV penicillin. She received a continuous infusion of 18 million U penicillin G daily for 10 days. Serial
sonograms showed improvement offetal ascites and pericardial effusion with 10 days of IV therapy,
and complete resolution of hydrops was noted within 3 weeks. The fetus was born at term with no
stigmata of congenital syphilis on newborn exam, and laboratory tests suggested adequate treatment
in utero
On the Impact of Re-Evaluation in 5G NR V2X Mode 2
5G NR V2X has been designed to support advanced connected and automated driving V2X services. These services are characterized by variable traffic patterns that can generate packet collisions in decentralized systems where vehicles autonomously select their radio resources like 5G NR V2X mode 2. 5G NR V2X introduces a re-evaluation mechanism at the MAC layer to detect and avoid possible packet collisions before a vehicle transmits in selected resources. Most of the studies conducted to date on 5G NR V2X do not consider the re-evaluation mechanism despite being a mandatory MAC feature. This article advances the state of the art with an in-depth analysis and evaluation of the operation and performance of re-evaluation in 5G NR V2X mode 2 under different traffic patterns and mode 2 configurations. The study shows that re-evaluation is effective in avoiding collisions with periodic traffic but its effectiveness decreases with aperiodic traffic and of variable size. The study also shows that re-evaluation is effective in avoiding collisions generated by the retransmission of packets. However, its overall impact on the performance of 5G NR V2X mode 2 is small, while it can have a relevant implementation cost due to the frequent re-evaluation checks and resource reselections. This raises questions on the current design of the re-evaluation mechanism that is a mandatory feature in 5G NR V2X mode 2
Direct evidence of lack of colocalisation of fluorescently labelled gold labels used in correlative light electron microscopy
Fluorescently labelled nanoparticles are routinely used in Correlative Light Electron Microscopy (CLEM) to combine the capabilities of two separate microscope platforms: fluorescent light microscopy (LM) and electron microscopy (EM). The inherent assumption is that the fluorescent label observed under LM colocalises well with the electron dense nanoparticle observed in EM. Herein we show, by combining single molecule fluorescent imaging with optical detection of the scattering from single gold nanoparticles, that for a commercially produced sample of 10 nm gold nanoparticles tagged to Alexa-633 there is in fact no colocalisation between the fluorescent signatures of Alexa-633 and the scattering associated with the gold nanoparticle. This shows that the attached gold nanoparticle quenches the fluorescent signal by ~95%, or less likely that the complex has dissociated. In either scenario, the observed fluorescent signal in fact arises from a large population of untagged fluorophores; rendering these labels potentially ineffective and misleading to the field
Towards a new generation axion helioscope
We study the feasibility of a new generation axion helioscope, the most
ambitious and promising detector of solar axions to date. We show that large
improvements in magnetic field volume, x-ray focusing optics and detector
backgrounds are possible beyond those achieved in the CERN Axion Solar
Telescope (CAST). For hadronic models, a sensitivity to the axion-photon
coupling of \gagamma\gtrsim {\rm few} \times 10^{-12} GeV is
conceivable, 1--1.5 orders of magnitude beyond the CAST sensitivity. If axions
also couple to electrons, the Sun produces a larger flux for the same value of
the Peccei-Quinn scale, allowing one to probe a broader class of models. Except
for the axion dark matter searches, this experiment will be the most sensitive
axion search ever, reaching or surpassing the stringent bounds from SN1987A and
possibly testing the axion interpretation of anomalous white-dwarf cooling that
predicts of a few meV. Beyond axions, this new instrument will probe
entirely unexplored ranges of parameters for a large variety of axion-like
particles (ALPs) and other novel excitations at the low-energy frontier of
elementary particle physics.Comment: 37 pages, 11 figures, accepted for publication in JCA
Rapid flow-sorting to simultaneously resolve multiplex massively parallel sequencing products
Sample preparation for Roche/454, ABI/SOLiD and Life Technologies/Ion Torrent sequencing are based on amplification of library fragments on the surface of beads prior to sequencing. Commonly, libraries are barcoded and pooled, to maximise the sequence output of each sequence run. Here, we describe a novel approach for normalization of multiplex next generation sequencing libraries after emulsion PCR. Briefly, amplified libraries carrying unique barcodes are prepared by fluorescent tagging of complementary sequences and then resolved by high-speed flow cytometric sorting of labeled emulsion PCR beads. The protocol is simple and provides an even sequence distribution of multiplex libraries when sequencing the flow-sorted beads. Moreover, since many empty and mixed emulsion PCR beads are removed, the approach gives rise to a substantial increase in sequence quality and mean read length, as compared to that obtained by standard enrichment protocols
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