Overall lipid compositions according to GC analyses.

<p>Class of neutral lipids (A) and types of fatty acyl-chains (B) were quantified and compared between the 8 samples according to GC analyses. Amounts are given in % of total. IBA+2MBA, relative amount of isobutyric and 2-methylbutyric acids; IVA, relative amount of isovaleric acid; ω3 FA, relative amount of omega3-fatty acids; Iso FA, relative amount of isobranched fatty acyl-chains; Linear FA, relative amount of linear fatty acyl-chains.</p

Assignment of the 24 major peaks detected by HR-MAS NMR in the blubber and melon of the harbour porpoise and long-finned pilot whale, and calculations used to determine peak area.

<p>Assignment of the 24 major peaks detected by HR-MAS NMR in the blubber and melon of the harbour porpoise and long-finned pilot whale, and calculations used to determine peak area.</p

Representative ¹H HR-MAS spectra of the samples.

<p>Intact tissues were placed in a zirconium oxide MAS rotor, D₂O was added for ²H field locking and ¹H HR-MAS NMR spectra were acquired at room temperature (spinning speed = 5000 Hz and ns = 64). The assignment of peaks <i>a</i> to <i>x</i> is given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0180597#pone.0180597.t001" target="_blank">Table 1</a>.</p

Overall lipid compositions according to HR-MAS NMR analyses.

<p>Class of neutral lipids (A) and types of fatty acyl-chains (B) were quantified and compared between the 8 samples according to HR-MAS NMR analyses. Amounts are given in % of total. IBA+2MBA, relative amount of isobutyric and 2-methylbutyric acids; IVA, relative amount of isovaleric acid; ω3 FA, relative amount of omega3-fatty acids; Iso FA, relative amount of isobranched fatty acyl-chains; Linear FA, relative amount of linear fatty acyl-chains.</p

WE profiling of the central melon and outer blubber from the long-finned pilot whale.

<p>WE from the central melon (A) and outer blubber (B) were purified by TLC before analysis by GC-MS. Note the different retention time windows between panel A and B. Identification of the major WE molecular species is provided in Supplemental <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0180597#pone.0180597.g003" target="_blank">Fig 3</a>.</p

Figure 1B

gif files for rotational views of 3D antigenic maps in Figure

Figure 1A

gif files for rotational views of 3D antigenic maps in Figure

Figure 1D

gif files for rotational views of 3D antigenic maps in Figure

Supplemental Table 2. Source data

Supplemental Table 2.Source data Text infiles, phylogenetic trees and source script for antigenic distance analyse

Figure 2. Source data 2

Figure 2. Source data 2 - Input and output text files used to calculate H1 and H3 comparative rates of antigenic evolution and diversity shown in Figure 2 and Supplementary File 3_ Table