Polimorphism screening using a vntr molecular marker system in romanian resident bison individuals

Genomic DNA isolated from the blood sampled from 14 individuals of Bison bonasus was used in order to obtain a molecular fingerprint and based on that, to obtain a dendrogram exhibiting the phylogentic relationship among those individuals. By using the PCR technique and an VNTR ( Variabile Number of Tandem Repeats molecular markers system: ISSR (Inter simple sequence repeats) was used in this study for establish set of DNA fingerprints. Based on molecular data a common binary matrix was developed. By using DendroUPGMA software, based on Jaccard coefficient the similarity indices and the genetic distances were calculated. Based on those data set a dendrogram containig the phylogenetic relationship among bison exemplars, was generated. By analyzing the molecular data it was observed that the degree of polymorphism was either absent or very low especially in this case, our recorded data suggest that among Romanian resident Bison exemplars, there is a high level of genetic similarity meaning that the individuals are most probably close blood relatives

How Many Times Can One Go Back to the Drawing Board before the Accurate Diagnosis and Surgical Treatment of Glucagonoma?

Glucagonomas are neuroendocrine tumors (NETs) that arise from the alpha cells of the pancreatic islets. They are typically slow-growing tumors associated with abnormal glucagon secretion, resulting in one or more non-specific clinical features, such as necrolytic migratory erythema (NME), diabetes, diarrhea, deep vein thrombosis, weight loss, and depression. Here, we report the case of a 44-year-old male with a history of diabetes mellitus, presenting with a pruritic and painful disseminated cutaneous eruption of erythematous plaques, with scales and peripheral pustules, misdiagnosed as disseminated pustular psoriasis and treated for 2 years with oral retinoid and glucocorticoids. During this period, the patient complained of weight loss of 32 kg and diarrhea and developed deep vein thrombosis. These symptoms, together with an inadequate response to therapy of the skin lesions, led to the reassessment of the initial diagnosis. Laboratory tests confirmed elevated plasma glucagon levels (>1000 pg/mL) and computed tomography (CT) scans revealed a 35/44 mm tumor in the pancreatic tail. Due to considerable disease complications and the COVID-19 pandemic, the surgical removal of the tumor was delayed for nearly 2 years. During this time, somatostatin analogue therapy efficiently controlled the glucagonoma syndrome and likely prevented tumor progression. As in other functional pancreatic NETs, the early clinical recognition of hormonal hypersecretion syndrome and the multidisciplinary approach are the keys for best patient management