Marrow-derived stromal cell delivery on fibrin microbeads can correct radiation-induced wound-healing deficits.

Skin that is exposed to radiation has an impaired ability to heal wounds. This is especially true for whole-body irradiation, where even moderate nonlethal doses can result in wound-healing deficits. Our previous attempts to administer dermal cells locally to wounds to correct radiation-induced deficits were hampered by poor cell retention. Here we improve the outcome by using biodegradable fibrin microbeads (FMBs) to isolate a population of mesenchymal marrow-derived stromal cells (MSCs) from murine bone marrow by their specific binding to the fibrin matrix, culture them to high density in vitro, and deliver them as MSCs on FMBs at the wound site. MSCs are retained locally, proliferate in site, and assist wounds in gaining tensile strength in whole-body irradiated mice with or without additional skin-only exposure. MSC-FMBs were effective in two different mouse strains but were ineffective across a major histocompatability barrier. Remarkably, irradiated mice whose wounds were treated with MSC-FMBs showed enhanced hair regrowth, suggesting indirect effect on the correction of radiation-induced follicular damage. Further studies showed that additional wound-healing benefit could be gained by administration of granulocyte colony-stimulating factor and AMD3100. Collagen strips coated with haptides and MSCs were also highly effective in correcting radiation-induced wound-healing deficits

Mitigation of Lethal Radiation Syndrome in Mice by Intramuscular Injection of 3D Cultured Adherent Human Placental Stromal Cells.

Exposure to high lethal dose of ionizing radiation results in acute radiation syndrome with deleterious systemic effects to different organs. A primary target is the highly sensitive bone marrow and the hematopoietic system. In the current study C3H/HeN mice were total body irradiated by 7.7 Gy. Twenty four hrs and 5 days after irradiation 2×10(6) cells from different preparations of human derived 3D expanded adherent placental stromal cells (PLX) were injected intramuscularly. Treatment with batches consisting of pure maternal cell preparations (PLX-Mat) increased the survival of the irradiated mice from ∼27% to 68% (P<0.001), while cell preparations with a mixture of maternal and fetal derived cells (PLX-RAD) increased the survival to ∼98% (P<0.0001). The dose modifying factor of this treatment for both 50% and 37% survival (DMF50 and DMF37) was∼1.23. Initiation of the more effective treatment with PLX-RAD injection could be delayed for up to 48 hrs after irradiation with similar effect. A delayed treatment by 72 hrs had lower, but still significantly effect (p<0.05). A faster recovery of the BM and improved reconstitution of all blood cell lineages in the PLX-RAD treated mice during the follow-up explains the increased survival of the cells treated irradiated mice. The number of CD45+/SCA1+ hematopoietic progenitor cells within the fast recovering population of nucleated BM cells in the irradiated mice was also elevated in the PLX-RAD treated mice. Our study suggests that IM treatment with PLX-RAD cells may serve as a highly effective "off the shelf" therapy to treat BM failure following total body exposure to high doses of radiation. The results suggest that similar treatments may be beneficial also for clinical conditions associated with severe BM aplasia and pancytopenia

Marrow-derived stromal cell delivery on fibrin microbeads can correct radiation-induced wound-healing deficits.

Skin that is exposed to radiation has an impaired ability to heal wounds. This is especially true for whole-body irradiation, where even moderate nonlethal doses can result in wound-healing deficits. Our previous attempts to administer dermal cells locally to wounds to correct radiation-induced deficits were hampered by poor cell retention. Here we improve the outcome by using biodegradable fibrin microbeads (FMBs) to isolate a population of mesenchymal marrow-derived stromal cells (MSCs) from murine bone marrow by their specific binding to the fibrin matrix, culture them to high density in vitro, and deliver them as MSCs on FMBs at the wound site. MSCs are retained locally, proliferate in site, and assist wounds in gaining tensile strength in whole-body irradiated mice with or without additional skin-only exposure. MSC-FMBs were effective in two different mouse strains but were ineffective across a major histocompatability barrier. Remarkably, irradiated mice whose wounds were treated with MSC-FMBs showed enhanced hair regrowth, suggesting indirect effect on the correction of radiation-induced follicular damage. Further studies showed that additional wound-healing benefit could be gained by administration of granulocyte colony-stimulating factor and AMD3100. Collagen strips coated with haptides and MSCs were also highly effective in correcting radiation-induced wound-healing deficits

Fibrin Microbeads Loaded with Mesenchymal Cells Support Their Long-Term Survival While Sealed at Room Temperature

Efficient transfer of progenitor cells without affecting their survival is a key factor in any practical cell therapy. Fibrin microbeads (FMB) were developed as hard biodegradable cell carriers. The FMB could efficiently isolate mesenchymal stem cells (MSCs) from different sources and support the expansion of matrix-dependent cell types in a three-dimensional culture in slow rotation. The cells on FMB could also undergo induced differentiation for their eventual implantation to enhance tissue regeneration. FMB loaded with isolated human MSC (hMSC) were sealed in tubes topped up with medium. Almost full cell survival was recorded when the sealed cells were maintained in room temperature for up to 10 days, followed by a recovery period of 24 hrs at optimal conditions. Assay of cells recovery after such long room temperature storage showed ∼80%–100% survival of the cells on FMB, with only a marginal survival of cells that were kept in suspension without FMB in the same conditions. The hMSC that survived storage at room temperature preserved their profile of mesenchymal cell surface markers, their rate of proliferation, and their differentiation potential. The cell protective effect was not dependent on the presence of serum in the storage medium. It was clearly shown that over-expression of hypoxia induced factor-1α in hMSC with time, which may have protected the sealed cells on FMB at room temperature storage, was not necessarily related to extreme hypoxic stress. Foreskin normal fibroblasts on FMB sealed at room temperature were similarly protected, but with no elevation of their hypoxia-induced factor-1α expression. The results also show that FMB, unlike other commercially available cell carriers, could be used for delivery and shipping of progenitor cells at room temperature for extended time intervals. This could be highly useful for cell transfer for therapeutic application and for simplified cell transfer between different research centers

The effect of IM PLX-RAD injection on the survival of 7.7 Gy total body irradiated mice.

<p>The setup of the experiment arms is described in (A). PLX-RAD cells (2×10⁶) were injected IM twice, 1^st injection was done 24 or 48 or 72 hrs and 2^nd injection was done on day 5 after irradiation. In another arm only a single injection on day 5 was given. The data presented are based on merged separate experimental repetitions. The total number of animals in each arm is shown in the legend. The follow-up of mice survival between the different schedules of treatment is shown in (B). The BM and blood cells profile of the surviving mice at the end of the experiments on day 23 is shown in (C). Significance: * = P<0.05, ** = P<0.01, **** = P<0.001, ***** = P<0.0001.</p

Evaluation of the dose modifying factor (DMF) of IM treatment with PLX-RAD.

<p>The ratio between the total dose needed to reach 50% survival rate of animals treated with PLX-RAD cells (9.05 Gy) relative to the dose needed to yield the same effect in the controls (7.42 Gy) was calculated based on the survival of irradiated mice exposed to different doses of radiation +/− IM injection of 2×10⁶ PLX cells at days 1 and 5 after irradiation. A DMF of 1.22 was recorded. The highest supported total body irradiation dose (HSD) which still yields 100% survival in the irradiated controls can be extrapolated from this Figure to be ∼6.8 Gy.</p

Kinetics of whole BM cellularity in mice treated on days 1 and 5 after irradiation with IM injections of PLX-RAD or vehicle controls.

<p>The whole BM cells were extracted from the tibias and femurs of the surviving treated animals from the 2 experimental arms on days 2, 6, 9, 14 and 23 after irradiation. Each group consisted of 8–10 mice. A clear advantage of the total cell number in the PLX-RAD injected mice was recorded along the follow up and was most significant from day 9 onward (A). In parallel samples of histological sections of decalcified bones from PLX-RAD and vehicle treated animals relative to bones from non-irradiated mice on day 9 and 23 are presented (B-G), further demonstrating the advantage of the PLX-RAD treatment in this critical time points. The histological bone sections are presented from day 9 for a non-irradiated control (B1 with magnified frames in B2 and B3), from vehicle controls (C1 with magnified frames in C2 and C3), and PLX-RAD treated mice (D1 with magnified frames in D2 and D3). The histological sections show only marginal better cellularity in the PLX-RAD treated mice on day 9 relative to vehicle controls. On day 23 after irradiation the difference was maximal, as also reflected in the relevant histological slides (F_1–3 and G_1–3) similar to the cellularity observed in non-irradiated control mice (E_1–3). Significance: ** = p<0.01, ***** = p<0.0001.</p

Time dependent alterations of the profile of the 3 peripheral blood cell lineages following irradiation and PLX-RAD treatment.

<p>Mice were injected with PLX-RAD on day 1 and 5 following 7.7 Gy irradiation. The mice were bled and sacrificed on days 2, 6, 9, 14, 23 and 30 after irradiation. RBC (A), WBC (B) and Platelets (C) were counted in peripheral blood. The data are based on at least 8 mice which were included for each group for the different rime points. For day 23 the data are based on 31 PLX-RAD treated and 55 vehicle treated mice (from repeated experiments). The recovery on day 23 of peripheral blood cells counts of the surviving mice subjected to the different tested arms with different timing of the first IM injections is presented. The more affected animals died before the end of the experiment, mostly in the group of vehicle treated controls, leaving only the few survivors on which the relevant data are based. The profile of the WBC, platelets and RBC are presented. Significance: * = P<0.05, ** = P<0.01, **** = P<0.001, ***** = P<0.0001.</p

The effect of IM PLX-Mat and PLX-RAD injection on the survival of 7.7 Gy total body irradiated mice.

<p>The setup of the experiment is described in (A). 2×10⁶ PLX-RAD or PLX-Mat cells were injected IM twice, 1^st injection delivered 24 hrs and 2^nd injection on day 5 after irradiation. The follow-up of mice survival is shown in (B). Weight changes for the group of IM injected mice at day 1 and 5 after irradiation are presented in (C). The BM and blood counts of the surviving animal at the end of the experiment at day 23 are presented in (D). Significance: * = P<0.05, ** = P<0.01*** = P<0.005, **** = P<0.001, ***** = P<0.0001.</p

The effect of IM injection of PLX-RAD cells on days 1 and 5 after radiation on the number of nucleated cells from whole BM and the ratio of progenitors within this cell population.

<p>(A) Up to day 9 after irradiation, the number of the nucleated BM cells decreased sharply in both arms, followed by a faster gain in the number of total nucleated BM cells in the PLX-RAD treated animals, as compared to the vehicle controls. By day 30 a full recovery of the number of nucleated BM cells was recorded only in the group of irradiated PLX-RAD treated mice. (B) FACS analysis of the kinetics of changes in the % of CD45⁺/Sca-1⁺ of the nucleated cells (representing the progenitor cells population) was tested in both arms of mice irradiated by 7.7 Gy, PLX-RAD or vehicle control treated. Maximal increase in the proportion of these cells due to the radiation exposure was recorded on day 9 in both groups, before the onset of the critical phase of the hematopoietic syndrome. Significance: * = P<0.05, ** = P<0.01.</p