GPR126 is a specifier of blood-brain barrier formation in the mouse central nervous system

Data availability. RNA sequencing data are available through the European Molecular Biology Laboratory–European Bioinformatics Institute (EMBL-EBI) ArrayExpress BioStudies portal with accession number E-MTAB-13914. All supporting data are provided in the Supporting Data Values file.The blood-brain barrier (BBB) acquires unique properties to regulate neuronal function during development. The formation of the BBB, which occurs in tandem with angiogenesis, is directed by the Wnt/β-catenin signaling pathway. Yet the exact molecular interplay remains elusive. Our study reveals the G protein–coupled receptor GPR126 as a critical target of canonical Wnt signaling, essential for the development of the BBB’s distinctive vascular characteristics and its functional integrity. Endothelial cell–specific deletion of the Gpr126 gene in mice induced aberrant vascular morphogenesis, resulting in disrupted BBB organization. Simultaneously, heightened transcytosis in vitro compromised barrier integrity, resulting in enhanced vascular permeability. Mechanistically, GPR126 enhanced endothelial cell migration, pivotal for angiogenesis, acting through an interaction between LRP1 and β1 integrin, thereby balancing the levels of β1 integrin activation and recycling. Overall, we identified GPR126 as a specifier of an organotypic vascular structure, which sustained angiogenesis and guaranteed the acquisition of the BBB properties during development.This study was supported by the European Research Council (ERC; 742922) and AIRC (18683 and 21320) to ED; ITN BtRAIN (675619) and CARIPLO Foundation (2016-0461) to MG; and AIRC (26183) and Horizon 2020 (964481) to DI. KB was funded by the Francis Crick Institute (FC001751). AP is funded by the Engineering and Physical Sciences Research Council (EP/S030964/1). SS was supported by the ERC (grant 101002280) and AIRC (grant 24415). MDG is supported by the Armenise Harvard Foundation, ERC (101116224), and AIRC (27564). MI is supported by the ERC (725038), AIRC (19891 and 22737), and Italian Ministry for University and Research (INF-ACT and PRIN 2022FMESXL)

Evidence from d+Au measurements for final-state suppression of high-p(T) hadrons in Au plus Au collisions at RHIC

We report measurements of single-particle inclusive spectra and two-particle azimuthal distributions of charged hadrons at high transverse momentum (high p(T)) in minimum bias and central d+Au collisions at roots(NN)=200 GeV. The inclusive yield is enhanced in d+Au collisions relative to binary-scaled p+p collisions, while the two-particle azimuthal distributions are very similar to those observed in p+p collisions. These results demonstrate that the strong suppression of the inclusive yield and back-to-back correlations at high p(T) previously observed in central Au+Au collisions are due to final-state interactions with the dense medium generated in such collisions

ALP production through non-linear Compton scattering in intense fields

23 pages, 14 figuresWe derive production yields for massive pseudo-scalar and scalar axion-like-particles (ALPs), through non-linear Compton scattering of an electron in the background of low- and high-intensity electromagnetic fields. In particular, we focus on electromagnetic fields from Gaussian plane wave laser pulses. A detailed study of the angular distributions and effects of the scalar and pseudo-scalar masses is presented. It is shown that ultra-relativistic seed electrons can be used to produce scalars and pseudo-scalars with masses up to the order of the electron mass. We briefly discuss future applications of this work towards lab-based searches for light beyond-the-Standard-Model particles

Incident energy dependence of p(t) correlations at relativistic energies

Journals published by the American Physical Society can be found at http://publish.aps.org/We present results for two-particle transverse momentum correlations, , as a function of event centrality for Au+Au collisions at root SNN = 20, 62, 130, and 200 GeV at the BNL Relativistic Heavy Ion Collider. We observe correlations decreasing with centrality that are similar at all four incident energies. The correlations multiplied by the multiplicity density increase with incident energy, and the centrality dependence may show evidence of processes such as thermalization, jet production, or the saturation of transverse flow. The square root of the correlations divided by the event-wise average transverse momentum per event shows little or no beam energy dependence and generally agrees with previous measurements made at the CERN Super Proton Synchrotron

Search for B-s(0) - GT mu(+)mu(-) and B-0 - GT mu(+)mu(-) Decays in pp Collisions at root s=7 TeV

A search for the rare decays B-s(0) -- GT mu(+)mu(-) and B-0 -- GT mu(+)mu(-) is performed in pp collisions at root s = 7 TeV, with a data sample corresponding to an integrated luminosity of 1.14 fb(-1), collected by the CMS experiment at the LHC. In both cases, the number of events observed after all selection requirements is consistent with expectations from background and standard-model signal predictions. The resulting upper limits on the branching fractions are B(B-s(0) -- GT mu(+)mu(-)) LT 1.9 x 10(-8) and B(B-0 -- GT mu(+)mu(-)) LT 4.6 x 10(-9), at 95% confidence level

Three-dimensional and immune electron microscopic analysis of the secretory pathway in Saccharomyces cerevisiae

Until now, the mechanisms of ER-to-Golgi and intra-Golgi transport remain obscure. This is especially evident for the Golgi of S. cerevisiae where different Golgi compartments are not organized in stacks. Here, using improved sample preparation protocols, we examined the 3D organization of pre-Golgi and Golgi compartments and found several new features of the structures functioning along the secretory pathway. In the cytoplasmic sheet ER, we found narrow pores that aggregated near the rims, and tubular networks tightly interconnected with sheets of several cytoplasmic ER cisternae. Within the Golgi compartments, we found disks with wide pores, disks with narrow pores, and disk-like networks with varicosities or nodules at the point of branching and thick membranes. Sometimes, these compartments contained 30 nm buds coated with a clathrin-like coat. The lumen of these Golgi compartments was more osmiophilic than the lumen of the ER. In contrast to ER elements, Golgi compartments were isolated and in the majority of cases not connected, although we observed some connections between Golgi compartments and also between Golgi disks with wide pores and the ER. Two types of free vesicles of 35-40 and 45-50 nm were found, the former being sometimes partially coated with a clathrin-like coat. Sec31, a COPII component, was found near narrow pores in the cytoplasmic sheets of the ER, over edge aggregates of narrow pores, and within the ER network. The cis-Golgi marker Rer1p was detected on disks or semi-spheres with wide pores, while the medial Golgi marker Gos1p was found on disks or semi-spheres with narrow pores. Gos1p was found to be enriched on 45-50 nm vesicles, while Rer1p was depleted. The 35-40 nm vesicles did not show either label. These findings are discussed from the point of view of mechanisms of transport