Different freezing factors associated with Saskatchewan winters in relation to the viability of Penicillium bilaiae

Non-Peer ReviewedSurvival of Penicillium bilaiae over the winter months is essential for use on fall seeded canola crops. Through a series of freeze experiments it is has been demonstrated that P. bilaiae can successfully survive low temperatures down to -196 °C when there is a controlled decrease in temperature. Penicillium bilaiae is still viable after being frozen at -20 °C for 100 days. Cycles of freezes to -8 °C and thaws at 2 °C had no effect on viability of P. bilaiae. Penicillium bilaiae on seed should be able to successfully survive freezing conditions associated with a Saskatchewan winter

Effect of seeding date, environment, and storage on canola seed vigour

Non-Peer ReviewedSeed vigour has been identified as one of the leading factors limiting stand establishment and yield in western Canada. Field studies at Scott, SK demonstrated that seed derived from Fall and April-sown canola produced higher plant densities, higher biomass at bolting, and higher seed yield than seed derived from May-sown canola. This study established the impact of seeding date on seed quality and vigour, which in turn affected emergence, seedling vigour and yield. Also, seed vigour slowly declines within one year, primarily from seed derived from the May-sown canola. Currently we are in the process of uncovering which genes and proteins are in common with vigour irrespective of seed source. We will combine our analysis with synchrotron technologies for a much more in-depth understanding of what constitutes “seed vigour” to develop a rapid, simple, and inexpensive method that will identify intrinsic characteristics of superior seed lots, as well as seed lots that lose vigour when stored under adverse conditions. In addition, we have initiated a study to compare hormones and metabolites during cold acclimation and freeze-induced injury/recovery to correlate these changes with winter survival. This research will identify traits that can be used in marker-assisted/molecular breeding programs for winter hardiness and possible genetic engineering studies on abiotic stress tolerance of seeds and plants. To further understand the processes involved in stress tolerance, we utilized gene transfer techniques to produce a PNT canola that over-expresses a novel gene which results in higher yields under stressful conditions. These PNT lines were tested in the field over 3 years across Western Canada in non-stressed, moderately stressed, or severely stressed areas. At each location, several lines flowered and matured 1 to 3 weeks earlier. The faster maturating PNT lines (up to 55% more mature at harvest) had increased yields (up to 32% increase) and enhanced seed quality (up to 87% increase in larger and more mature seed) versus the control. These results, both in controlled laboratory tests and in field trials, have been optimistic for genetic engineering of plants for enhanced stress tolerance without losing agronomical important characteristics

Update on fall dormant seeded canola research

Non-Peer Reviewe

The Effect of Water, Sugars, and Proteins on the Pattern of Ice Nucleation and Propagation in Acclimated and Nonacclimated Canola Leaves

Infrared video thermography was used to observe ice nucleation temperatures, patterns of ice formation, and freezing rates in nonacclimated and cold acclimated leaves of a spring (cv Quest) and a winter (cv Express) canola (Brassica napus). Distinctly different freezing patterns were observed, and the effect of water content, sugars, and soluble proteins on the freezing process was characterized. When freezing was initiated at a warm subzero temperature, ice growth rapidly spread throughout nonacclimated leaves. In contrast, acclimated leaves initiated freezing in a horseshoe pattern beginning at the uppermost edge followed by a slow progression of ice formation across the leaf. However, when acclimated leaves, either previously killed by a slow freeze (2°C h(−1)) or by direct submersion in liquid nitrogen, were refrozen their freezing pattern was similar to nonacclimated leaves. A novel technique was developed using filter paper strips to determine the effects of both sugars and proteins on the rate of freezing of cell extracts. Cell sap from nonacclimated leaves froze 3-fold faster than extracts from acclimated leaves. The rate of freezing in leaves was strongly dependent upon the osmotic potential of the leaves. Simple sugars had a much greater effect on freezing rate than proteins. Nonacclimated leaves containing high water content did not supercool as much as acclimated leaves. Additionally, wetted leaves did not supercool as much as nonwetted leaves. As expected, cell solutes depressed the nucleation temperature of leaves. The use of infrared thermography has revealed that the freezing process in plants is a complex process, reminding us that many aspects of freezing tolerance occur at a whole plant level involving aspects of plant structure and metabolites rather than just the expression of specific genes alone

Seed priming enhances germination of annual caraway (Carum carvi L.) at low temperatures

Non-Peer Reviewe

The effect of stubble on the growth and cold hardiness of ‘Norstar’ winter wheat

Non-Peer Reviewe

Late fall and early spring seeding of herbicide tolerant canola

Non-Peer Reviewe