Comparison of a Flow Assay for Brucellosis Antibodies with the Reference cELISA Test in West African Bos indicus

Brucellosis is considered by the Food and Agricultural Organisation and the World Health Organisation as one of the most widespread zoonoses in the world. It is a major veterinary public health challenge as animals are almost exclusively the source of infection for people. It is often undiagnosed in both human patients and the animal sources and it is widely acknowledged that the epidemiology of brucellosis in humans and animals is poorly understood, particularly in sub-Saharan Africa. It is therefore important to develop better diagnostic tools in order to improve our understanding of the epidemiology and also for use in the field for disease control and eradication. As with any new diagnostic test, it is essential that it is validated in as many populations as possible in order to characterise its performance and improve the interpretation of its results. This paper describes a comparison between a new lateral flow assasy (LFA) for bovine brucellosis and the widely used cELISA in a no gold standard analysis to estimate test performance in this West African cattle population. A Bayesian formulation of the Hui-Walter latent class model incorporated previous studies' data on sensitivity and specificity of the cELISA. The results indicate that the new LFA is very sensitive (∼87%) and highly specific (∼97%). The analysis also suggests that the current cut-off of the cELSIA may not be optimal for this cattle population but alternative cut-offs did not significantly change the estimates of the LFA. This study demonstrates the potential usefulness of this simple to use test in field based surveillance and control which could be easily adopted for use in developing countries with only basic laboratory facilities

Nuclear brinkmanship: a study in non-linguistic communication

This article examines meaning making with nuclear bombs and military manoeuvres. The data is verbatim audio recordings from the White House during the Cuban Missile Crisis. The analysis uses concepts from impression management and dialogism. It is found that actions often speak louder than words and that even non-linguistic communication with nuclear weapons is often oriented to third-parties, in this case, world opinion. A novel process of 'staging the other' is identified, that is, when one side tries to create a situation which will force the other side to act in a way which will create a negative impression on world opinion. Staging the other is a subtle form of meaning making for it entails shaping how third parties will view a situation without those third parties being aware of the intentionality of the communication