The relationship of natural killer cell counts, perforin mRNA and CD2 expression to post-exercise natural killer cell activity in humans

The aim of this study was to further investigate the mechanism of suppression of natural killer (NK) cell cytotoxic activity In peripheral blood following strenuous exercise. Blood was collected for analysis of NK cell concentration, cytotoxic activity, CD2 surface expression and perforin gene expression from runners (RUN, n = 6) and resting controls (CONTROL, n = 4) pre-exercise, 0, 1.5, 5, and 24 h following a 60-min treadmill run at 80% of VO2 peak. Natural killer cytotoxic activity, measured using a whole blood chromium release assay, fluctuated minimally in the CONTROL group and increased by 63% and decreased by 43% 0 and 1.5 h post-exercise, respectively, in the RUN group (group x time, P < 0.001). Lytic index (cytotoxic activity per cell) did not change. Perforin mRNA, measured using quantitative real-time polymerase chain reaction (ORT-PCR) decreased from pre- to post-exercise and remained decreased through 24 h, The decrease from pre- to 0 In post-exercise was seen predominately in the RUN group and was inversely correlated r = - 0.95) to pre-exercise perform mRNA. The NK cell surface expression of CD2 (lymphocyte function-associated antigen-2) was determined using fluorescent antibodies and flow cytometry, There was no change in the proportion of NK cells expressing CD2 or CD2 density, We conclude that (1) numerical redistribution accounted for most of the change in NK cytotoxic activity following a strenuous run, (2) decrease in perforin gene expression during the run was inversely related to pre-exercise levels but did not parallel changes in cytotoxic activity, and (3) CD2 surface expression was not affected by exercise

Designing Policy for Reducing the Off-farm Effects of Soil Erosion Using Choice Experiments

Soil erosion produces both on-site private costs and off-site social costs, such as desertification, rural depopulation, siltation of waterways and reductions in biodiversity. To design efficient policies, land use planners and decision makers need information on the relative weights of changes in these consequences, since policy alternatives, such as different management restrictions, will have varying impacts on these consequences of erosion. The research presented here uses the choice experiment method to evaluate these relative weights, using a case study in the Alto Genil and Guadajoz watersheds in southern Spain. We find that reductions in desertification, protection of water quality, protection of biodiversity, the area covered by the scheme, and the number of rural jobs safeguarded are all significant determinants of preferences over alternative policy designs

Parcelamento e largura da faixa de aplicação da uréia na recuperação do nitrogênio pela planta de milho Splitting and width of strip urea applications and the recovery of nitrogen by maize plants

Com o objetivo de estudar o parcelamento e a largura da faixa de aplicação como meios para aumentar a recuperação pelo milho do nitrogênio da uréia aplicada em superfície, foi conduzido no ano agrícola 93/94, em um Latossolo Vermelho Escuro, em condições de campo, um experimento delineado em blocos ao acaso, com cinco repetições. Sete tratamentos foram utilizados: testemunha (sem N em cobertura); aplicação de N parcelada (50 kg ha-1 de N aos 38 dias após semeadura - d.a.s e 50 kg ha-1 de N aos 60 d.a.s.) para faixas de 10, 20 e 40 cm; aplicação de N (100 kg ha-1 de N aos 38 d.a.s.) para faixas de 10 e 40 cm e, aplicação de N parcelada (50 kg de N/ha aos 38 d.a.s. e 50 kg ha-1 de N aos 60 d.a.s.) para faixa de 20 cm de largura, neste caso, uréia granulada. A aplicação de uréia parcelada em cobertura, em faixas de 10, 20 e 40 cm de largura, não afetaram a massa de matéria seca, o conteúdo, a quantidade e a recuperação do N pela planta de milho. A recuperação de N pela planta de milho foi maior para a aplicação de 220 kg ha-1 de uréia em uma única aplicação em faixas de 10cm de largura em relação a de 40 cm. O parcelamento da uréia aumentou os valores de massa seca, conteúdo, quantidade e recuperação do N na planta de milho em relação a aplicação em uma única vez.<br>In order to evaluate the nitrogen recovery by maize, following a surface application of urea, a trial was carried out during 1993/94 under field conditions, on a dark red latosol (oxisol). A randomized complete block statistical design was used, with seven treatments and five replications. The seven treatments were: control (no N); three split N applications (50 kg N ha-1 urea at 38 and 60 days after sowing) using 10, 20 or 40 cm width strips; two single N applications (100 kg N ha-1 urea at 38 days after sowing) using 10 or 40 cm width strips; and split N applications (50 kg N ha-1 urea at 38 and 60 days after sowing) using a 20 cm width strip (granular urea). The top dressing that was applied at two growth stages in 10, 20 or 40 cm width strips did not affect dry matter production, content, amount and recovery of N in the corn plant. Only when one urea application was made, the recovery of N from the corn plant was larger for the 10 cm of width in relation to 40 cm. The split application increased significantly dry matter production, content, amount and recovery of N in the corn plant in one single application