Salinity's influence on boron toxicity in broccoli: II. Impacts on boron uptake, uptake mechanisms and tissue ion relations

Limited research has been conducted on the interactive effects of salinity and boron stresses on plants despite their common occurrence in natural systems. The purpose of this research was to determine and quantify the interactive effects of salinity, salt composition and boron on broccoli (Brassica oleracea L.) performance, particularly, element accumulation, ion interactions and boron uptake processes. A greenhouse experiment was conducted using a sand tank system where salinity-B treatment solutions were supplemented with a complete nutrient solution. Chloride-dominated salinity and salinity characteristic of California's San Joaquin valley (SJV), or sulfate-dominated, were tested at ECw (electrical conductivity of the irrigation water) levels of 2, 12 and 19 dS m-1. Each salinity treatment consisted of boron treatments of 0.5, 12 and 24 mg L-1. Salinity, regardless of salt composition, reduced shoot boron concentration at very high boron concentration (24 mg L-1). However, increased salinity increased shoot boron concentration when external boron concentration was low (0.5 mg L-1). Tissue Ca, Mg, Na, K, S and Cl concentrations were also affected by salinity level, chloride or sulfate salinity composition, and in some cases by substrate boron concentration. Calcium concentrations in shoots were greater for chloride-treated salinity as compared to SJV salinity-treated plants; magnesium concentrations trended opposite and were greater in those treated with SJV salinity. Chloride and sodium shoot concentrations both increased with salinity. Shoot chloride was greater with chloride substrate salinity and shoot sodium was greater with SJV substrate salinity. Using stable isotope analysis of solutions to separate transpiration from evapotranspiration (ET), we found that boron uptake and accumulation in the shoot was not simply the product of mass flow (solution concentration × cumulative transpiration), and the vast majority of the water lost from the tank system was by transpiration (>90%) regardless of treatment. Under low substrate boron, the levels of boron in broccoli shoots could be not accounted for by simple passive uptake and transport in the transpiration stream, which suggests that some energy-dependent process was also occurring. However, under high boron treatments, broccoli plants exhibited a mechanism that restricted boron uptake, transport and accumulation in the shoot.Active uptake Combined stressors Mineral ion interactions Oxygen isotopes Passive uptake Transpiration

Evaluation of soil salinity leaching requirement guidelines

Water for irrigation is a major limitation to agricultural production in many parts of the world. Use of waters with elevated levels of salinity is one likely option to meet the supply of increased demands. The sources of these waters include drainage water generated by irrigated agriculture, municipal wastewater, and poor quality groundwater. Soil salinity leaching requirements that were established several decades ago were based on steady-state conditions. Recently transient-state models have been developed that potentially can more correctly predict the dynamics of the chemical-physical-biological interactions in an agricultural system. The University of California Center for Water Resources appointed a workgroup to review the development of steady-state analyses and transient-state models, and to determine whether the current recommended guidelines for leaching requirement based on steady-state analyses need to be revised. The workgroup concludes that the present guidelines overestimate the leaching requirement and the negative consequences of irrigating with saline waters. This error is particularly large at low leaching fractions. This is a fortuitous finding because irrigating to achieve low leaching fractions provides a more efficient use of limited water supplies.Irrigation Salt leaching Steady-state analyses Transient-state models