1,643 research outputs found
A microscopic description and ultrastructural characterisation of Dientamoeba fragilis: An emerging cause of human enteric disease
Dientamoeba fragilis is a pathogenic trichomonad found in the gastrointestinal tract of humans and is implicated as a cause of diarrhoea. Despite its discovery over a century ago, there has been no recent thorough description of this parasite by microscopy. Scanning electron microscopy, transmission electron microscopy, confocal and light microscopy were therefore used to characterise D. fragilis populations growing in xenic culture. Two different populations - smooth and ruffled cells - were identifiable by scanning electron microscopy. No flagella, pelta structures, undulating membrane or pseudocyst-like forms were present. The organelles in D. fragilis were analysed by transmission electron microscopy; like Trichomonas and Histomonas, D. fragilis contains hydrogenosomes that presumably represent the site of anaerobic respiration. The nuclear morphology of D. fragilis trophozoites grown in vitro and trophozoites from clinical isolates were also compared by confocal microscopy and light microscopy. The majority of cells grown in culture were mononucleate while most cells in permanent stained faecal smears were binucleate. The two nuclei of D. fragilis are morphologically indistinguishable and contain equivalent amounts of DNA as determined by DAPI staining. The approximate cell and nuclear volume of four isolates of D. fragilis were measured and shown to be comparable to other trichomonads. In addition, the discovery of a virus-like particle is reported, to our knowledge for the first time in D. fragilis. This study therefore provides extensive and novel details of the ultrastructure of a neglected protozoan parasite that is an emerging cause of human disease. © 2011 Australian Society for Parasitology Inc
Newly defined conditions for the in vitro cultivation and cryopreservation of Dientamoeba fragilis: New techniques set to fast track molecular studies on this organism
Dientamoeba fragilis is a pathogen of the human gastrointestinal tract that is a common cause of diarrhoea. A paucity of knowledge on the in vitro cultivation and cryopreservation of Dientamoeba has meant that few studies have been conducted to investigate its biology. The objective of this study was to define, for the first time, in vitro culture conditions able to support the long-term in vitro growth of Dientamoeba. Also, we aimed to define a suitable method for cryopreserving viable Dientamoeba trophozoites. A modified BD medium, TYGM-9, Loeffler's slope medium, Robinson's medium, Medium 199, Trichosel and a Tritrichomonas fetus medium were compared, using cell counts, for their ability to support the growth of D. fragilis at various temperatures and atmospheric conditions. Loeffler's slope medium supported significantly better growth compared to other media. A temperature of 42C and a microaerophilic atmosphere were also optimum for Dientamoeba growth. To our knowledge, this is the first study to describe and compare different culture media and conditions for the growth of clinical isolates of D. fragilis. This new technology will aid the development of diagnostics for dientamoebiasis as well as facilitate large-scale sequencing projects that will fast track molecular studies on D. fragilis. © Cambridge University Press 2010
Superluminal neutrinos in long baseline experiments and SN1987a
Precise tests of Lorentz invariance in neutrinos can be performed using long
baseline experiments such as MINOS and OPERA or neutrinos from astrophysical
sources. The MINOS collaboration reported a measurement of the muonic neutrino
velocities that hints to super-luminal propagation, very recently confirmed at
6 sigma by OPERA. We consider a general parametrisation which goes beyond the
usual linear or quadratic violation considered in quantum-gravitational models.
We also propose a toy model showing why Lorentz violation can be specific to
the neutrino sector and give rise to a generic energy behaviour E^alpha, where
alpha is not necessarily an integer number. Supernova bounds and the preferred
MINOS and OPERA regions show a tension, due to the absence of shape distortion
in the neutrino bunch in the far detector of MINOS. The energy independence of
the effect has also been pointed out by the OPERA results.Comment: 22 pages, 7 figures; comment on Cherenkov emission added, version
matching JHEP published pape
Higgs and non-universal gaugino masses: no SUSY signal expected yet?
So far, no supersymmetric particles have been detected at the Large Hadron
Collider (LHC). However, the recent Higgs results have interesting implications
for the SUSY parameter space. In this paper, we study the consequences of an
LHC Higgs signal for a model with non-universal gaugino masses in the context
of SU(5) unification. The gaugino mass ratios associated with the higher
representations produce viable spectra that are largely inaccessible to the
current LHC and direct dark matter detection experiments. Thus, in light of the
Higgs results, the non-observation of SUSY is no surprise.Comment: supplementary file containing plots with log priors in ancillary
files. v2: added some comments on more general settings and references,
accepted for publication in JHE
Challenges of Profile Likelihood Evaluation in Multi-Dimensional SUSY Scans
Statistical inference of the fundamental parameters of supersymmetric
theories is a challenging and active endeavor. Several sophisticated algorithms
have been employed to this end. While Markov-Chain Monte Carlo (MCMC) and
nested sampling techniques are geared towards Bayesian inference, they have
also been used to estimate frequentist confidence intervals based on the
profile likelihood ratio. We investigate the performance and appropriate
configuration of MultiNest, a nested sampling based algorithm, when used for
profile likelihood-based analyses both on toy models and on the parameter space
of the Constrained MSSM. We find that while the standard configuration is
appropriate for an accurate reconstruction of the Bayesian posterior, the
profile likelihood is poorly approximated. We identify a more appropriate
MultiNest configuration for profile likelihood analyses, which gives an
excellent exploration of the profile likelihood (albeit at a larger
computational cost), including the identification of the global maximum
likelihood value. We conclude that with the appropriate configuration MultiNest
is a suitable tool for profile likelihood studies, indicating previous claims
to the contrary are not well founded.Comment: 21 pages, 9 figures, 1 table; minor changes following referee report.
Matches version accepted by JHE
Process evaluation of appreciative inquiry to translate pain management evidence into pediatric nursing practice
Background
Appreciative inquiry (AI) is an innovative knowledge translation (KT) intervention that is compatible with the Promoting Action on Research in Health Services (PARiHS) framework. This study explored the innovative use of AI as a theoretically based KT intervention applied to a clinical issue in an inpatient pediatric care setting. The implementation of AI was explored in terms of its acceptability, fidelity, and feasibility as a KT intervention in pain management.
Methods
A mixed-methods case study design was used. The case was a surgical unit in a pediatric academic-affiliated hospital. The sample consisted of nurses in leadership positions and staff nurses interested in the study. Data on the AI intervention implementation were collected by digitally recording the AI sessions, maintaining logs, and conducting individual semistructured interviews. Data were analysed using qualitative and quantitative content analyses and descriptive statistics. Findings were triangulated in the discussion.
Results
Three nurse leaders and nine staff members participated in the study. Participants were generally satisfied with the intervention, which consisted of four 3-hour, interactive AI sessions delivered over two weeks to promote change based on positive examples of pain management in the unit and staff implementation of an action plan. The AI sessions were delivered with high fidelity and 11 of 12 participants attended all four sessions, where they developed an action plan to enhance evidence-based pain assessment documentation. Participants labeled AI a 'refreshing approach to change' because it was positive, democratic, and built on existing practices. Several barriers affected their implementation of the action plan, including a context of change overload, logistics, busyness, and a lack of organised follow-up.
Conclusions
Results of this case study supported the acceptability, fidelity, and feasibility of AI as a KT intervention in pain management. The AI intervention requires minor refinements (e.g., incorporating continued follow-up meetings) to enhance its clinical utility and sustainability. The implementation process and effectiveness of the modified AI intervention require evaluation in a larger multisite study
Molecular dynamics simulations of oscillatory Couette flows with slip boundary conditions
The effect of interfacial slip on steady-state and time-periodic flows of
monatomic liquids is investigated using non-equilibrium molecular dynamics
simulations. The fluid phase is confined between atomically smooth rigid walls,
and the fluid flows are induced by moving one of the walls. In steady shear
flows, the slip length increases almost linearly with shear rate. We found that
the velocity profiles in oscillatory flows are well described by the Stokes
flow solution with the slip length that depends on the local shear rate.
Interestingly, the rate dependence of the slip length obtained in steady shear
flows is recovered when the slip length in oscillatory flows is plotted as a
function of the local shear rate magnitude. For both types of flows, the
friction coefficient at the liquid-solid interface correlates well with the
structure of the first fluid layer near the solid wall.Comment: 31 pages, 11 figure
The moment of truth for WIMP Dark Matter
We know that dark matter constitutes 85% of all the matter in the Universe,
but we do not know of what it is made. Amongst the many Dark Matter candidates
proposed, WIMPs (weakly interacting massive particles) occupy a special place,
as they arise naturally from well motivated extensions of the standard model of
particle physics. With the advent of the Large Hadron Collider at CERN, and a
new generation of astroparticle experiments, the moment of truth has come for
WIMPs: either we will discover them in the next five to ten years, or we will
witness the inevitable decline of WIMP paradigm.Comment: To appear in Nature (Nov 18, 2010
Flavour physics constraints in the BMSSM
We study the implications of the presence of the two leading-order,
non-renormalizable operators in the Higgs sector of the MSSM to flavour physics
observables. We identify the constraints of flavour physics on the parameters
of the BMSSM when we: a) focus on a region of parameters for which electroweak
baryogenesis is feasible, b) use a CMSSM-like parametrization, and c) consider
the case of a generic NUHM-type model. We find significant differences as
compared to the standard MSSM case.Comment: 22 pages, 7 figure
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