Reduction of myocardial infarction by postischemic administration of the calpain inhibitor A-705253 in comparison to the Na(+)/H(+) exchange inhibitor Cariporide (R) in isolated perfused rabbit hearts

The calpain inhibitor A-705253 and the Na(+)/H(+) exchange inhibitor Cariporide (R) were studied in isolated perfused rabbit hearts subjected to 60 min occlusion of the ramus interventricularis of the left coronary artery (below the origin of the first diagonal branch), followed by 120 min of reperfusion. The inhibitors were added to the perfusion fluid solely or in combination at the beginning of reperfusion. Hemodynamic monitoring and biochemical analysis of perfusion fluid from the coronary outflow were performed. Myocardial infarct size and area at risk (transiently not perfused myocardium) were determined from left ventricular slices after a special staining procedure with Evans blue and 2,3,5-triphenyltetrazolium chloride. The infarcted area (dead myocardium) was 72.7 +/- 4.0% of the area at risk in untreated controls, but was significantly smaller in the presence of the inhibitors. The largest effect was observed with 10(-6) M A-705253, which reduced the infarcted area to 49.2 +/- 4.1% of the area at risk, corresponding to a reduction of 33.6%. Cariporide (R) at 10(-6) M reduced the infarct size to the same extent. The combination of both inhibitors, however, did not further improve cardioprotection. No significant difference was observed between the experimental groups in coronary perfusion, left ventricular pressure, heart rate, or in the release of lactate dehydrogenase and creatine kinase from heart muscle

The irreversibility line of overdoped Bi_{2+x}Sr_{2-(x+y)}Cu_{1+y}O_{6 +- delta} at ultra-low temperatures and high magnetic fields

The irreversible magnetization of the layered high-T_{c} superconductor Bi_{2+x}Sr_{2-(x+y)}Cu_{1+y}O_{6 +- delta} (Bi-2201) has been measured by means of a capacitive torquemeter up to B=28 T and down to T=60 mK. No magnetization jumps, peak effects or crossovers between different pinning mechanisms appear to be present. The deduced irreversibility field B_{irr} can not be described by the law B_{irr}(T)=B_{irr}(0)(1-T/T_{c})^n based on flux creep, but an excellent agreement is found with the analytical form of the melting line of the flux lattice as calculated from the Lindemann criterion. The behavior of B_{irr}(T) obtained here is very similar to the resistive critical field of a Bi-2201 thin film, suggesting that magnetoresistive experiments are likely to be strongly influenced by flux lattice melting.Comment: 4 pages, 4 eps figure