Serum biochemical parameters and cytokine profiles associated with natural African trypanosome infections in cattle.

BACKGROUND: Animal African trypanosomiasis (AAT) greatly affects livestock production in sub-Saharan Africa. In Ghana prevalence of AAT is estimated to range between 5 and 50%. Studies have reported serum biochemical aberrations and variability in cytokine profiles in animals during infection. However, information regarding the biochemical parameters and cytokine profiles associated with natural infections are limited. This study was therefore aimed at investigating changes in the levels of serum biochemical parameters and inflammatory cytokines during a natural infection. METHODS: Nested internal transcribed spacer (ITS)-based PCR and sequencing were used to characterise trypanosome infection in cattle at two areas in Ghana (Adidome and Accra) of different endemicities. The cattle were sampled at four to five-week intervals over a period of six months. Levels of serum biochemical parameters, including creatinine, cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALT), total bilirubin and total protein and cytokines (interleukin 10, interleukin 4, interleukin 12, interferon gamma and tumor necrosis factor alpha) were measured in serum samples and then compared between infected cattle and uninfected controls. RESULTS: The predominant trypanosome species detected in Accra (non-endemic) and Adidome (endemic) were Trypanosoma theileri and Trypanosoma vivax, respectively. Serum biochemical parameters were similar between infected and uninfected cattle in Accra. Infected cattle at Adidome however, had significantly higher levels of ALP, creatinine, total protein and total bilirubin (P < 0.05) and significantly lower levels of cholesterol (P < 0.05) at specific time points. At basal levels and during infection, significantly higher pro-inflammatory to anti-inflammatory (Th1/Th2) cytokine ratios were observed in cattle at Adidome compared to Accra (P < 0.05), indicating a shift towards Th1 immune response in Adidome. Levels of IL-10 were, however, significantly elevated in infected cattle in Accra (P < 0.05), suggesting high anti-inflammatory cytokine response in Accra. CONCLUSION: These results suggests that cattle in an endemic area repeatedly infected with trypanosomes of different species or different antigenic types demonstrate high pro-inflammatory (Th1) immune response and biochemical alterations whereas cattle in a non-endemic area with predominantly chronic T. theileri infections demonstrate high anti-inflammatory response and no biochemical alterations

Social network analysis in tourism services distribution channels

Application of network analysis in tourism research is relatively new, especially in the study of tourism services distribution channels. Network analysis is employed to investigate the structure and pattern of relationships between actors in a network. This paper applies NA with Organizational Risk Analysis software to analyse tourism services distribution channels. The data is collected froma major tour operator in Hanoi and its travel agent network. The results of the research show the pattern of the network between tour operators and travel agencies; and between tour operators. Network analysis also reveals the cooperation and cohesion of the network as well as the network dynamics between the case study tour operator and its travel agencies over a period of time

“If I had to pick any subject, it wouldn’t be maths”: foundations for engagement with mathematics during the middle years

This article is a report on a longitudinal case study that investigated the problem of lowered engagement with mathematics and students’ perspectives of the factors that influenced their engagement during the middle years of schooling. The article provides a synthesis of the entire study and a summary of its findings. In order to address the research question a group of 20 students from within the same school cohort participated in the study spanning three school years from their final year of primary school, to their second year of secondary school. Data was collected through interviews, focus group discussions, and classroom observations. A major finding of this study was that positive pedagogical relationships between teachers and their students must be developed as a foundation for sustained engagement