9 research outputs found
The Use of Fluorescence Resonance Energy Transfer (FRET) to Measure Axon Growth and Guidance-Related Intracellular Signalling in Live Dorsal Root Ganglia Neuronal Growth Cones
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Simultaneous assessment of cAMP signaling events in different cellular compartments using FRET-based reporters
No full textRecording Intracellular cAMP Levels with EPAC-Based FRET Sensors by Fluorescence Lifetime Imaging
No full textsiFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data
No full textWe developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180 degrees -phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients
