7 research outputs found
Permeability properties of a three-cell type in vitro model of blood-brain barrier.
We previously found that RBE4.B brain capillary endothelial cells (BCECs) form a layer with blood-brain barrier (BBB) properties if co-cultured with neurons for at least one week. As astrocytes are known to modulate BBB functions, we further set a culture system that included RBE4.B BCECs, neurons and astrocytes. In order to test formation of BBB, we measured the amount of 3H-sucrose able to cross the BCEC layer in this three-cell type model of BBB. Herein we report that both neurons and astrocytes induce a decrease in the permeability of the BCEC layer to sucrose. These effects are synergic as if BCECs are cultured with both neurons and astrocytes for 5 days, permeability to sucrose decreases even more. By Western analysis, we also found that, in addition to the canonical 60 kDa occludin, anti-occludin antibodies recognize a smaller protein of 48 kDa which accumulates during rat brain development. Interestingly this latter protein is present at higher amounts in endothelial cells cultured in the presence of both astrocytes and neurons, that is in those conditions in which sucrose permeation studies indicate formation of BBB
Neuronal factors secreted through unusual pathways are probably involved in blood brain barrier (BBB) formation
Effetto dei terreni condizionati da neuroni ed astrociti sulle proprietà barriera di cellule di capillari cerebrali.
Profilo patogenetico e sensibilità ai farmaci di stipiti di S.aureus meticillino-resistente SCCMEC tipo IVa isolati in quattro ospedali palermitani
The Effects of Triggering Mechanisms on the Energy Absorption Capability of Circular Jute/Epoxy Composite Tubes under Quasi-Static Axial Loading
The usage of composite materials have been improving over the years due to its
superior mechanical properties such as high tensile strength, high energy absorption capability,
and corrosion resistance. In this present study, the energy absorption capability of circular jute/
epoxy composite tubes were tested and evaluated. To induce the progressive crushing of the
composite tubes, four different types of triggering mechanisms were used which were the nontrigger, single chamfered trigger, double chamfered trigger and tulip trigger. Quasi-static axial
loading test was carried out to understand the deformation patterns and the load-displacement
characteristics for each composite tube. Besides that, the influence of energy absorption, crush
force efficiency, peak load, mean load and load-displacement history were examined and
discussed. The primary results displayed a significant influence on the energy absorption
capability provided that stable progressive crushing occurred mostly in the triggered tubes
compared to the non-triggered tubes. Overall, the tulip trigger configuration attributed the
highest energy absorptio