Host‐Response Therapeutics for Periodontal Diseases

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141161/1/jper1592.pd

Periodontal Surveillance – Prospects for the Future

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141014/1/jper1365.pd

Periodontal Surveillance – Implications in the Promotion of Public Health

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142113/1/jper1177.pd

Enhancing Periodontal Health Through Regenerative Approaches

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142301/1/jpers1.pd

Epigenetics and Its Role in Periodontal Diseases: A State‐of‐the‐Art Review

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141896/1/jper0556.pd

Soft and hard tissue augmentation procedures for promotion of peri‐implant health and aesthetics

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/142542/1/clr13102.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/142542/2/clr13102_am.pd

Platelet‐Derived Growth Factor (PDGF) Gene Delivery for Application in Periodontal Tissue Engineering

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141045/1/jper0815.pd

When epigenetics meets bioengineering—A material characteristics and surface topography perspective

The field of tissue engineering and regenerative medicine (TE/RM) involves regeneration of tissues and organs using implantable biomaterials. The term epigenetics refers to changes in gene expression that are not encoded in the DNA sequence, leading to remodeling of the chromatin and activation or inactivation of gene expression. Recently, studies have demonstrated that these modifications are influenced not only by biological cues but also by mechanical and topographical signals. This review highlights the current knowledge on emerging approaches in TE/RM with a focus on the effect of materials and topography on the epigenetic expression pattern in cells with potential impacts on modulating regenerative biology. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2065–2071, 2018.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/144628/1/jbmb33953.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/144628/2/jbmb33953_am.pd

Gene Therapy of Bone Morphogenetic Protein for Periodontal Tissue Engineering

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141217/1/jper0202.pd

TLR4, NOD1 and NOD2 mediate immune recognition of putative newly identified periodontal pathogens

Periodontitis is a polymicrobial inflammatory disease that results from the interaction between the oral microbiota and the host immunity. While the innate immune response is important for disease initiation and progression, the innate immune receptors that recognize both classical and putative periodontal pathogens that elicit an immune response have not been elucidated. By using the Human Oral Microbe Identification Microarray (HOMIM), we identified multiple predominant oral bacterial species in human plaque biofilm that strongly associate with severe periodontitis. Ten of the identified species were evaluated in greater depth, 6 being classical pathogens and 4 putative novel pathogens. Using human peripheral blood monocytes (HPBM) and murine bone marrow–derived macrophages (BMDM) from wild-type (WT) and toll-like receptor (TLR)-specific and MyD88 knockouts (KOs), we demonstrated that heat-killed Campylobacter concisus, Campylobacter rectus, Selenomonas infelix, Porphyromonas endodontalis, Porphyromonas gingivalis, and Tannerella forsythia mediate high immunostimulatory activity. C. concisus, C. rectus, and S. infelix exhibited robust TLR4 stimulatory activity. Studies using mesothelial cells from WT and NOD1-specific KOs and NOD2-expressing human embryonic kidney (HEK) cells demonstrated that Eubacterium saphenum, Eubacterium nodatum and Filifactor alocis exhibit robust NOD1 stimulatory activity, and that Porphyromonas endodontalis and Parvimonas micra have the highest NOD2-stimulatory activity. These studies allowed us to provide important evidence on newly-identified putative pathogens in periodontal disease pathogenesis showing that these bacteria exhibit different immunostimulatory activity via TLR4, NOD1, and NOD2 (Clinicaltrials.gov {"type":"clinical-trial","attrs":{"text":"NCT01154855","term_id":"NCT01154855"}}NCT01154855)