Segmentation Of Stretched Pap Smear Cytology Images Using Clustering Algorithm.

Papanicolaou test or better known as Pap test is the most popular and effective screening test for cervical cancer. At time, however, the detection of abnormal or cancerous cervical cells can be missed due to technical and human errors

Effect of processing parameters on properties of bitumen emulsions

Bitumen emulsions are special type of paving binder materials used in road construction industry due to its environmentally friendly approach, cost efficiency and less energy consumption compared to other conventional bitumen binders. Despite so many benefits, the use of bitumen emulsions is still limited in many regions due to difficulties faced in its emulsification process. Controlling several process parameters are needed during formulation while achieving the sufficient storage stability and viscosity. Therefore, this study was conducted to investigate the effect of different processing parameters on the properties of bitumen emulsions in terms of storage stability and viscosity. The 80/100 grade bitumen was emulsified through colloid mill, considering different pH values and emulsifier concentrations as processing parameters during emulsification process. The results indicate that the storage stability has improved significantly with increased concentration of emulsifier whereas high acidic system in terms of pH level has also substantially enhanced storage stability at lower level of emulsifier concentration. In addition, the viscosity of bitumen emulsions has also been affected by the emulsifier concentration and higher amount of emulsifier resulted in higher values of viscosity at lower pH level. The data achieved through this investigation can be further used to study the effect of other formulation parameters on properties of bitumen emulsions

The Fermi accelerator in atom optics

We study the classical and quantum dynamics of a Fermi accelerator realized by an atom bouncing off a modulated atomic mirror. We find that in a window of the modulation amplitude dynamical localization occurs in both position and momentum. A recent experiment [A. Steane, P. Szriftgiser, P. Desbiolles, and J. Dalibard, Phys. Rev. Lett. {\bf 74}, 4972 (1995)] shows that this system can be implemented experimentally.Comment: 5 pages, 5 figure

The empirical approach to strengthen coordinated cadastral database accuracy

The coordinated cadastral database in Malaysia is known as National Digital Cadastral Database (NDCDB) with an expected accuracy of ±10cm in rural and ±5cm in urban area. Till date, there are approximately 7.8 million land parcels and 22 million boundary markers in the NDCDB for the whole of Peninsular Malaysia and Federal Territory of Labuan covering total area of 132,183 km2. Since 2010, NDCDB block adjustment has been carried out continuously without giving prime concern to eliminate gross errors in the adjustment's input data. This approach aims to propose a methodology to improve the positional accuracy of the existing NDCDB through utilisation of the current eKadaster application. A comprehensive investigation in the office and field processes has been carried out to prove the efficiency of the methodology introduced. This investigation was focused on the East Coast Rail Link (ECRL) right of way (ROW) survey from Dungun to Besut where displacement of 1 to 6 meters relative to the NDCDB coordinates, as shown in the Land Acquisition (LA) Plan, have been identified. Areas involved are coded as Block T10701, T1100101 and T1100102 which are located in Lubuk Kawah and Pelagat Sub-districts, in the state of Terengganu. Positional accuracy of the NDCDB after adjustment was further verified by comparing the coordinates of randomly picked ground proofing points in the field using Real-Time Kinematic (RTK) observation. This will determine the Root Mean Square Error (RMSE) of the respective NDCDB Block based on actual observations and adjusted coordinate values. With that, it can be concluded that the proposed approach is reasonably practical and capable to improve and strengthen the positional accuracy of existing coordinated cadastral database used in Malaysia

What Is the Optimal Therapy for Patients with H5N1 Influenza?

Nicholas White discusses optimal dosing of oseltamivir, Robert Webster and Elena Govorkova discuss combination antiviral therapy, and Timothy Uyeki discusses clinical care of patients with H5N1

Molecular Dynamics Simulations Suggest that Electrostatic Funnel Directs Binding of Tamiflu to Influenza N1 Neuraminidases

Oseltamivir (Tamiflu) is currently the frontline antiviral drug employed to fight the flu virus in infected individuals by inhibiting neuraminidase, a flu protein responsible for the release of newly synthesized virions. However, oseltamivir resistance has become a critical problem due to rapid mutation of the flu virus. Unfortunately, how mutations actually confer drug resistance is not well understood. In this study, we employ molecular dynamics (MD) and steered molecular dynamics (SMD) simulations, as well as graphics processing unit (GPU)-accelerated electrostatic mapping, to uncover the mechanism behind point mutation induced oseltamivir-resistance in both H5N1 “avian” and H1N1pdm “swine” flu N1-subtype neuraminidases. The simulations reveal an electrostatic binding funnel that plays a key role in directing oseltamivir into and out of its binding site on N1 neuraminidase. The binding pathway for oseltamivir suggests how mutations disrupt drug binding and how new drugs may circumvent the resistance mechanisms

Influenza virus infection among pediatric patients reporting diarrhea and influenza-like illness

<p>Abstract</p> <p>Background</p> <p>Influenza is a major cause of morbidity and hospitalization among children. While less often reported in adults, gastrointestinal symptoms have been associated with influenza in children, including abdominal pain, nausea, vomiting, and diarrhea.</p> <p>Methods</p> <p>From September 2005 and April 2008, pediatric patients in Indonesia presenting with concurrent diarrhea and influenza-like illness were enrolled in a study to determine the frequency of influenza virus infection in young patients presenting with symptoms less commonly associated with an upper respiratory tract infection (URTI). Stool specimens and upper respiratory swabs were assayed for the presence of influenza virus.</p> <p>Results</p> <p>Seasonal influenza A or influenza B viral RNA was detected in 85 (11.6%) upper respiratory specimens and 21 (2.9%) of stool specimens. Viable influenza B virus was isolated from the stool specimen of one case. During the time of this study, human infections with highly pathogenic avian influenza A (H5N1) virus were common in the survey area. However, among 733 enrolled subjects, none had evidence of H5N1 virus infection.</p> <p>Conclusions</p> <p>The detection of influenza viral RNA and viable influenza virus from stool suggests that influenza virus may be localized in the gastrointestinal tract of children, may be associated with pediatric diarrhea and may serve as a potential mode of transmission during seasonal and epidemic influenza outbreaks.</p

Influenza Virus Respiratory Infection and Transmission Following Ocular Inoculation in Ferrets

While influenza viruses are a common respiratory pathogen, sporadic reports of conjunctivitis following human infection demonstrates the ability of this virus to cause disease outside of the respiratory tract. The ocular surface represents both a potential site of virus replication and a portal of entry for establishment of a respiratory infection. However, the properties which govern ocular tropism of influenza viruses, the mechanisms of virus spread from ocular to respiratory tissue, and the potential differences in respiratory disease initiated from different exposure routes are poorly understood. Here, we established a ferret model of ocular inoculation to explore the development of virus pathogenicity and transmissibility following influenza virus exposure by the ocular route. We found that multiple subtypes of human and avian influenza viruses mounted a productive virus infection in the upper respiratory tract of ferrets following ocular inoculation, and were additionally detected in ocular tissue during the acute phase of infection. H5N1 viruses maintained their ability for systemic spread and lethal infection following inoculation by the ocular route. Replication-independent deposition of virus inoculum from ocular to respiratory tissue was limited to the nares and upper trachea, unlike traditional intranasal inoculation which results in virus deposition in both upper and lower respiratory tract tissues. Despite high titers of replicating transmissible seasonal viruses in the upper respiratory tract of ferrets inoculated by the ocular route, virus transmissibility to naïve contacts by respiratory droplets was reduced following ocular inoculation. These data improve our understanding of the mechanisms of virus spread following ocular exposure and highlight differences in the establishment of respiratory disease and virus transmissibility following use of different inoculation volumes and routes

The N-Terminal Region of the PA Subunit of the RNA Polymerase of Influenza A/HongKong/156/97 (H5N1) Influences Promoter Binding

BACKGROUND: The RNA polymerase of influenza virus is a heterotrimeric complex of PB1, PB2 and PA subunits which cooperate in the transcription and replication of the viral genome. Previous research has shown that the N-terminal region of the PA subunit of influenza A/WSN/33 (H1N1) virus is involved in promoter binding. METHODOLOGY/PRINCIPAL FINDINGS: Here we extend our studies of the influenza RNA polymerase to that of influenza strains A/HongKong/156/97 (H5N1) and A/Vietnam/1194/04 (H5N1). Both H5N1 strains, originally isolated from patients in 1997 and 2004, showed significantly higher polymerase activity compared with two classical human strains, A/WSN/33 (H1N1) and A/NT/60/68 (H3N2) in vitro. This increased polymerase activity correlated with enhanced promoter binding. The N-terminal region of the PA subunit was the major determinant of this enhanced promoter activity. CONCLUSIONS/SIGNIFICANCE: Overall we suggest that the N-terminal region of the PA subunit of two recent H5N1 strains can influence promoter binding and we speculate this may be a factor in their virulence

Generation, Characterization and Epitope Mapping of Two Neutralizing and Protective Human Recombinant Antibodies against Influenza A H5N1 Viruses

The development of new therapeutic targets and strategies to control highly pathogenic avian influenza (HPAI) H5N1 virus infection in humans is urgently needed. Broadly cross-neutralizing recombinant human antibodies obtained from the survivors of H5N1 avian influenza provide an important role in immunotherapy for human H5N1 virus infection and definition of the critical epitopes for vaccine development.We have characterized two recombinant baculovirus-expressed human antibodies (rhAbs), AVFluIgG01 and AVFluIgG03, generated by screening a Fab antibody phage library derived from a patient recovered from infection with a highly pathogenic avian influenza A H5N1 clade 2.3 virus. AVFluIgG01 cross-neutralized the most of clade 0, clade 1, and clade 2 viruses tested, in contrast, AVFluIgG03 only neutralized clade 2 viruses. Passive immunization of mice with either AVFluIgG01 or AVFluIgG03 antibody resulted in protection from a lethal H5N1 clade 2.3 virus infection. Furthermore, through epitope mapping, we identify two distinct epitopes on H5 HA molecule recognized by these rhAbs and demonstrate their potential to protect against a lethal H5N1 virus infection in a mouse model.Importantly, localization of the epitopes recognized by these two neutralizing and protective antibodies has provided, for the first time, insight into the human antibody responses to H5N1 viruses which contribute to the H5 immunity in the recovered patient. These results highlight the potential of a rhAbs treatment strategy for human H5N1 virus infection and provide new insight for the development of effective H5N1 pandemic vaccines