Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis

Cellular decision-making is mediated by a complex interplay of external stimuli with the intracellular environment, in particular transcription factor regulatory networks. Here we have determined the expression of a network of 18 key haematopoietic transcription factors in 597 single primary blood stem and progenitor cells isolated from mouse bone marrow. We demonstrate that different stem/progenitor populations are characterized by distinctive transcription factor expression states, and through comprehensive bioinformatic analysis reveal positively and negatively correlated transcription factor pairings, including previously unrecognized relationships between Gata2, Gfi1 and Gfi1b. Validation using transcriptional and transgenic assays confirmed direct regulatory interactions consistent with a regulatory triad in immature blood stem cells, where Gata2 may function to modulate cross-inhibition between Gfi1 and Gfi1b. Single-cell expression profiling therefore identifies network states and allows reconstruction of network hierarchies involved in controlling stem cell fate choices, and provides a blueprint for studying both normal development and human disease

A Comparison of Recreational- and Intermediate Survey-Grade GPS Units for Importing Data into GIS Software Packages

Global positioning systems (GPS) and Geographic Information Systems (GIS) have become common tools for Extension professionals. These systems vary in terms of accuracy and cost. GPS ranges from recreational-quality to survey-quality, with intermediate levels in between. As the user moves from recreational-quality to survey-quality, both accuracy and cost increase. We compare a recreational GPS unit (Garmin) and importing data into Google Earth software and an intermediate survey-grade GPS unit (Trimble Juno) and importing data into ArcMap GIS software. These represent two of the most common GIS choices available to natural resource professionals. Cost effectiveness will depend on accuracy requirements

Living on the Edge: Residential Property Values in the Urban-Rural Interface

This study estimates the contribution of both urban-rural fringe location and lake proximity on residential property values in three upstate counties of South Carolina through estimation of spatial hedonic housing price models. Location in the urban fringe and the urban-rural interface are found to have a positive impact on residential housing values relative to either urban or more rural locations. Lakes in the upstate contribute positively to housing values to the extent that the house has a view of a lake, lake access, or lake frontage

Living on the Edge: Residential Property Values in the Urban-Rural Interface

This study estimates the contribution of both urban-rural fringe location and lake proximity on residential property values in three upstate counties of South Carolina through estimation of spatial hedonic housing price models. Location in the urban fringe and the urban-rural interface are found to have a positive impact on residential housing values relative to either urban or more rural locations. Lakes in the upstate contribute positively to housing values to the extent that the house has a view of a lake, lake access, or lake frontage.environmental valuation, hedonic analysis, lake proximity, rural development, urban-rural interface, urban sprawl, O18, R14, R21,