Towards an operational use of benthic foraminifera for organic pollution monitoring in open and enclosed marine environments: case histories from the outer shelf off Congo and the Firth of Clyde in Scotland.

Foraminifera are among the most abundant protists in marine benthic environments. Because of their short life cycles, high biodiversity and specific ecological requirements of individual species, foraminifera react quickly to environmental disturbance, and can be successfully employed as bio-indicators of environmental change, such as those brought about by anthropogenic pollution. In the last decennia, foraminifera have been increasingly used to monitor pollution in a wide range of marine environments, such as intertidal mudflats impacted by oil spillages, harbours affected by heavy metal pollution, or eutrophicated continental shelves. Our best examples of anthropogenic eutrophication are 1) a drill cutting disposal sites at the outer continental shelf off Congo, where we observed a zonation of foraminiferal faunas in the 750 m around the discharge point. In the immediate vicinity of the discharge points (within 70 m), faunas are characterised by low foraminiferal densities. Faunas between 70 m and 250 m of the disposal sites have very high foraminiferal densities, with high percentages of opportunistic taxa such as B. aculeata and B. marginata. Between 250 and 750 m, foraminiferal densities decrease, and the percentages of opportunistic species are lower; 2) a sewage sludge disposal on the sea floor in the Firth of Clyde (Scotland) where we used benthic foraminifera and macrofaunal/meiofaunal assemblages to evaluate the impact. These two communities present a very similar distributional pattern around the disposal site. In its immediate vicinity, both groups show impoverished faunas composed exclusively of species tolerant to strong oxygen depletion. This area is surrounded by an aureole of high density faunas dominated by opportunistic species. Still farther away, faunal density decreases, and equilibrium taxa gradually replace opportunistic species. At about 3 Km of the disposal site, both foraminiferal and macro-/meiofaunal taxa become comparable to those found at the reference station. We used these data to develop a quantitative pollution index, values of which are strongly correlated with the distance to the disposal site. This foraminiferal index offers the possibility to quantify the impact of anthropogenic eutrophication in marine environments, but its validity must be tested in wider range of naturally and anthropogenetically impacted marine environments.Foraminifera are among the most abundant protists in marine benthic environments. Because of their short life cycles, high biodiversity and specific ecological requirements of individual species, foraminifera react quickly to environmental disturbance, and can be successfully employed as bio-indicators of environmental change, such as those brought about by anthropogenic pollution. In the last decennia, foraminifera have been increasingly used to monitor pollution in a wide range of marine environments, such as intertidal mudflats impacted by oil spillages, harbours affected by heavy metal pollution, or eutrophicated continental shelves. Our best examples of anthropogenic eutrophication are 1) a drill cutting disposal sites at the outer continental shelf off Congo, where we observed a zonation of foraminiferal faunas in the 750 m around the discharge point. In the immediate vicinity of the discharge points (within 70 m), faunas are characterised by low foraminiferal densities. Faunas between 70 m and 250 m of the disposal sites have very high foraminiferal densities, with high percentages of opportunistic taxa such as B. aculeata and B. marginata. Between 250 and 750 m, foraminiferal densities decrease, and the percentages of opportunistic species are lower; 2) a sewage sludge disposal on the sea floor in the Firth of Clyde (Scotland) where we used benthic foraminifera and macrofaunal/meiofaunal assemblages to evaluate the impact. These two communities present a very similar distributional pattern around the disposal site. In its immediate vicinity, both groups show impoverished faunas composed exclusively of species tolerant to strong oxygen depletion. This area is surrounded by an aureole of high density faunas dominated by opportunistic species. Still farther away, faunal density decreases, and equilibrium taxa gradually replace opportunistic species. At about 3 Km of the disposal site, both foraminiferal and macro-/meiofaunal taxa become comparable to those found at the reference station. We used these data to develop a quantitative pollution index, values of which are strongly correlated with the distance to the disposal site. This foraminiferal index offers the possibility to quantify the impact of anthropogenic eutrophication in marine environments, but its validity must be tested in wider range of naturally and anthropogenetically impacted marine environments

Locus coeruleus modulates neuroinflammation in parkinsonism and dementia

Locus Coeruleus (LC) is the main noradrenergic nucleus of the central nervous system, and its neurons widely innervate the whole brain. LC is severely degenerated both in Alzheimer’s disease (AD) and in Parkinson’s disease (PD), years before the onset of clinical symptoms, through mechanisms that differ among the two disorders. Several experimental studies have shown that noradrenaline modulates neuroinflammation, mainly by acting on microglia/astrocytes function. In the present review, after a brief introduction on the anatomy and physiology of LC, we provide an overview of experimental data supporting a pathogenetic role of LC degeneration in AD and PD. Then, we describe in detail experimental data, obtained in vitro and in vivo in animal models, which support a potential role of neuroinflammation in such a link, and the specific molecules (i.e., released cytokines, glial receptors, including pattern recognition receptors and others) whose expression is altered by LC degeneration and might play a key role in AD/PD pathogenesis. New imaging and biochemical tools have recently been developed in humans to estimate in vivo the integrity of LC, the degree of neuroinflammation, and pathology AD/PD biomarkers; it is auspicable that these will allow in the near future to test the existence of a link between LC-neuroinflammation and neurodegeneration directly in patients

Benthic foraminifera as bio-indicators of drill cutting disposal in tropical east Atlantic outer shelf environments

We present a study of benthic foraminiferal faunas from the outer continental shelf off Congo (tropical West Africa), with the aim to determine the impact of the discharge of oily drill cuttings on the sea floor environment, to judge the regenerating capacity of the benthic ecosystem, and to investigate the possibility to develop an environmental monitoring method for open marine continental shelf environments, based on benthic foraminifera. We studied the spatial distribution and microhabitats of living and dead foraminiferal faunas, sampled in April 2003, 4 years after the end of disposal activities, in the upper 3 cm of the sediment at 9 stations (about 180 m depth) offshore Congo, that were subject to various degrees of pollution by oily cuttings from 1993 until 1999. Our results describe a zonation of foraminiferal faunas in the 750 m around the former disposal sites. At the immediate vicinity of the discharge points (within 70 m), faunas are characterized by low foraminiferal densities. Faunas between 70 m and 250 m of the disposal sites have very high foraminiferal densities, with high percentages (about 80%) of opportunistic taxa such as Bulimina aculeata, Bulimina marginata, Textularia sagittula, Trifarina bradyi and Bolivina spp. Between 250 and 750 m from the disposal site, foraminiferal densities decrease, and the percentages of opportunistic species are lower (40–60% of indicator species). These results show that 4 years after the cessation of oily cutting disposal, strong environmental impact is limited to the 250 m around the disposal sites. We used these data to develop a quantitative pollution index, values of which are strongly correlated to distance to the disposal site. This foraminiferal index offers the possibility to quantify the impact of anthropogenic eutrophication in continental shelf environments, but its validity must be tested in other continental shelf environments

Discrete Matter, Far Fields, and Dark Matter

We show that in cosmology the gravitational action of the far away matter has quite relevant effects, if retardation of the forces and discreteness of matter (with its spatial correlation) are taken into account. The expansion rate is found to be determined by the density of the far away matter, i.e., by the density of matter at remote times. This leads to the introduction of an effective density, which has to be five times larger than the present one, if the present expansion rate is to be accounted for. The force per unit mass on a test particle is found to be of the order of 0.2cH_0. The corresponding contribution to the virial of the forces for a cluster of galaxies is also discussed, and it is shown that it fits the observations if a decorrelation property of the forces at two separated points is assumed. So it appears that the gravitational effects of the far away matter may have the same order of magnitude as the corresponding local effects of dark matter.Comment: 16 pages, 1 figure. LaTex documen

Locus Coeruleus and neurovascular unit: From its role in physiology to its potential role in Alzheimer’s disease pathogenesis

Locus coeruleus (LC) is the main noradrenergic (NA) nucleus of the central nervous system. LC degenerates early during Alzheimer's disease (AD) and NA loss might concur to AD pathogenesis. Aside from neurons, LC terminals provide dense innervation of brain intraparenchymal arterioles/capillaries, and NA modulates astrocyte functions. The term neurovascular unit (NVU) defines the strict anatomical/functional interaction occurring between neurons, glial cells, and brain vessels. NVU plays a fundamental role in coupling the energy demand of activated brain regions with regional cerebral blood flow, it includes the blood–brain barrier (BBB), plays an active role in neuroinflammation, and participates also to the glymphatic system. NVU alteration is involved in AD pathophysiology through several mechanisms, mainly related to a relative oligoemia in activated brain regions and impairment of structural and functional BBB integrity, which contributes also to the intracerebral accumulation of insoluble amyloid. We review the existing data on the morphological features of LC-NA innervation of the NVU, as well as its contribution to neurovascular coupling and BBB proper functioning. After introducing the main experimental data linking LC with AD, which have repeatedly shown a key role of neuroinflammation and increased amyloid plaque formation, we discuss the potential mechanisms by which the loss of NVU modulation by LC might contribute to AD pathogenesis. Surprisingly, thus far not so many studies have tested directly these mechanisms in models of AD in which LC has been lesioned experimentally. Clarifying the interaction of LC with NVU in AD pathogenesis may disclose potential therapeutic targets for AD

The degeneration of locus coeruleus occurring during Alzheimer’s disease clinical progression: a neuroimaging follow-up investigation

\ua9 The Author(s) 2024.The noradrenergic nucleus Locus Coeruleus (LC) is precociously involved in Alzheimer’s Disease (AD) pathology, and its degeneration progresses during the course of the disease. Using Magnetic Resonance Imaging (MRI), researchers showed also in vivo in patients the disruption of LC, which can be observed both in Mild Cognitively Impaired individuals and AD demented patients. In this study, we report the results of a follow-up neuroradiological assessment, in which we evaluated the LC degeneration overtime in a group of cognitively impaired patients, submitted to MRI both at baseline and at the end of a 2.5-year follow-up. We found that a progressive LC disruption can be observed also in vivo, involving the entire nucleus and associated with clinical diagnosis. Our findings parallel neuropathological ones, which showed a continuous increase of neuronal death and volumetric atrophy within the LC with the progression of Braak’s stages for neurofibrillary pathology. This supports the reliability of MRI as a tool for exploring the integrity of the central noradrenergic system in neurodegenerative disorders

Relaxation properties in classical diamagnetism

It is an old result of Bohr that, according to classical statistical mechanics, at equilibrium a system of electrons in a static magnetic field presents no magnetization. Thus a magnetization can occur only in an out of equilibrium state, such as that produced through the Foucault currents when a magnetic field is switched on. It was suggested by Bohr that, after the establishment of such a nonequilibrium state, the system of electrons would quickly relax back to equilibrium. In the present paper, we study numerically the relaxation to equilibrium in a modified Bohr model, which is mathematically equivalent to a billiard with obstacles, immersed in a magnetic field that is adiabatically switched on. We show that it is not guaranteed that equilibrium is attained within the typical time scales of microscopic dynamics. Depending on the values of the parameters, one has a relaxation either to equilibrium or to a diamagnetic (presumably metastable) state. The analogy with the relaxation properties in the Fermi Pasta Ulam problem is also pointed out

Paraffin waxes in the North-Western Mediterranean Sea: A comprehensive assessment in the Pelagos Sanctuary, a Specially Protected Area of Mediterranean Importance

Paraffin waxes are widely recognized as emerging marine pollutants, even their classification by the recent monitoring programs and the knowledge of their occurrence, and sources of contamination in marine ecosystems are poorly defined and reported. Wax presence and distribution have been evaluated in different environmental compartments in the Pelagos Sanctuary (Mediterranean Sea) floating on the sea surface and stranded on beaches, focussing on their characterization, accumulation areas and pollution inputs. More than 2500 yellow paraffin residues were detected and analysed in the study area showing a prevailing dimension smaller than 5 mm. The Genoa Canyon and the waters facing Gorgona Island resulted in the more polluted areas representing two distinct hotspots of wax accumulation potentially related to the high density of tanker vessels sailing to and from the harbour of Genova and Livorno. Higher concentrations of beached particles were found along the Tuscan coast (11 items/100 m) and on Pianosa Island (110 items/m2). This study gives valuable insights into paraffin wax pollution in the Pelagos Sanctuary, emphasizing the need for harmonized monitoring and detection methods to elucidate the potential impacts on marine organisms. Moreover, mitigating actions are crucial to prevent and curb the waxes pollution of marine ecosystems

Benthic foraminifera as bio-indicators of eutrophicated environments

Foraminifera are among the most abundant protists in marine benthic environments (Murray, 1991). Because of their short life cycles, high biodiversity and specific ecological requirements of individual species, foraminifera react quickly to environmental disturbance, and can be successfully employed as bio-indicators of environmental change, such as those brought about by anthropogenic pollution (as defined by Kramer and Botterweg, 1991). Foraminiferal assemblages are easy to collect; foraminifera are commonly abundant, providing a highly reliable database for statistical analysis, even when only small sample volumes are available. Furthermore, many foraminiferal taxa secrete a carbonate shell, and leave an excellent fossil record, that may be used to characterise baseline conditions, and to reconstruct the state of the ecosystem prior to sampling. Studies of the effects of pollution on benthic foraminiferal assemblages, and their possible use as pollution indicators were initiated in the early 1960’s by Resig (1960) and Watkins (1961). In the last decennia, foraminifera have been increasingly used to monitor pollution in a wide range of marine environments, such as intertidal mudflats impacted by oil spillages (Morvan et al., 2004), harbours affected by heavy metal pollution (Armynot Du Châtelet et al., 2004), or eutrophicated continental shelves (Sharifi et al., 1991; Yanko and Flexer, 1991). The goal of our study is to compare different types of eutrophicated environments, under anthropogenic or natural conditions:  we used benthic foraminifera as bio-indicators of anthropogenic eutrophication caused by drill cutting discharges (Congo and Gabon), by sewage sludge (Firth of Clyde; Scotland) and by fish farms (Loch Etive; Scotland) and compared the faunal patterns with those observed in the Rhone prodelta, en environment characterised by strong natural eutrophication due to  important continental nutrient input