6 research outputs found

    Structural Basis for Enzymatic Excision of N1 Methyladenine and N3 Methylcytosine from DNA

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    N1 methyladenine m1A and N3 methylcytosine m3C are major toxic and mutagenic lesions induced by alkylation in single stranded DNA. In bacteria and mammals, m1A and m3C were recently shown to be repaired by AlkB mediated oxidative demethylation, a direct DNA damage reversal mechanism. No AlkB gene homologues have been identified in Archaea. We report that m1A and m3C are repaired by the AfAlkA base excision repair glycosylase of Archaeoglobus fulgidus, suggesting a different repair mechanism for these lesions in the third domain of life. In addition, AfAlkA was found to effect a robust excision of 1,N6 ethenoadenine. We present a high resolution crystal structure of AfAlkA, which, together with the characterization of several site directed mutants, forms a molecular rationalization for the newly discovered base excision activit

    Business Marketing Comes of Age: A Comprehensive Review of the Literature

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    Organic Chemicals

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