Total forearm blood flow as an indicator of skeletal muscle blood flow: effect of subcutaneous adipose tissue blood flow

Total forearm blood flow as an indicator of skeletal muscle blood flow: effect of subcutaneous adipose tissue blood flow. Blaak EE, van Baak MA, Kemerink GJ, Pakbiers MT, Heidendal GA, Saris WH. Department of Human Biology, University of Limburg, Maastricht, The Netherlands. 1. In studying forearm skeletal muscle substrate exchange, an often applied method for estimating skeletal muscle blood flow is strain gauge plethysmography. A disadvantage of this method is that it only measures total blood flow through a segment of forearm and not the flow through the individual parts such as skin, adipose tissue and muscle. 2. In the present study the contribution of forearm subcutaneous adipose tissue blood flow to total forearm blood flow was evaluated in lean (% body fat 17.0 +/- 2.2) and obese males (% body fat 30.9 +/- 1.6) during rest and during infusion of the non-selective beta-agonist isoprenaline. Measurements were obtained of body composition (hydrostatic weighing), forearm composition (magnetic resonance imaging) and of total forearm (venous occlusion plethysmography), skin (skin blood flow, laser Doppler), and subcutaneous adipose tissue blood flow (133Xe washout technique). 3. The absolute forearm area and the relative amount of fat (% of forearm area) were significantly higher in obese as compared to lean subjects, whereas the relative amounts of muscle and skin were similar. 4. During rest, the percentage contribution of adipose tissue blood flow to total forearm blood flow was significantly higher in lean compared with obese subjects (19 vs 12%, P < 0.05), whereas there were no differences in percentage contribution between both groups during isoprenaline infusion (10 vs 13%). Furthermore, the contribution of adipose tissue blood flow to total forearm blood flow was significantly lower during isoprenaline infusion than during rest in lean subjects (P < 0.05), whereas in the obese this value was similar during rest and during isoprenaline infusion.(ABSTRACT TRUNCATED AT 250 WORDS

Characteristics of a first generation x-ray system

Purpose: To compare the antiquated x-ray system of Hoffmans and van Kleef (circa 1896) with modern x-ray equipment in terms of radiation dose, x-ray beam properties, image quality, and electrical parameters. Materials and Methods: The antiquated x-ray system consisted of a Ruhmkorff inductor, battery, and Crookes tube. The radiation dose rate, x-ray beam properties, and electrical characteristics of this system were determined. A modern computed radiography plate was used to compare images of a hand specimen obtained by using the antiquated system with images obtained by using the modern system. Results: A peak voltage of 73 kV was obtained with an 8-V battery. With Crookes tube number 9, the half-value layer of the generated x-rays was 0.56 mm Al. Pinhole images showed that the x-rays originated from an extended area of the glass wall, causing image blurring. When measured on the skin of a hand specimen, the radiation dose of the antiquated system was about 10 times greater than that of the modern system for the same detector signal. The estimated skin dose was about 74 mGy for the antiquated system and 0.05 mGy for the modern system. The corresponding exposure times were 90 minutes and 21 msec. Conclusion: Radiation dose and exposure time of the antiquated system were greater than those of the modern system by about three and five orders of magnitude, respectively. Images of the hand specimen obtained with the antiquated system were severely blurred but were still awe inspiring, considering the simplicity of the system

Quantitative analysis of magnetization transfer images of the brain:Effect of closed head injury, age and sex on white matter

Magnetization transfer (MT) imaging has an application in quantitative assessment of cerebral white matter. Previously published postprocessing methods have inherent problems, and therefore a new analysis technique is presented. This technique was found to be more sensitive for white matter changes in patients with a postconcussional syndrome, compared to other methods previously described. Because of the potential application of this technique in longitudinal and group studies, age and sex dependence of the MT ratio (MTR) of white matter were studied. In a group of 51 healthy subjects, a decrease in the mean MTR as well as an increasing distribution width of the MTR was found with increasing age. The mean MTR in males was higher than in females. These results stress the need to take age and sex into account when interpreting MTR data