17 research outputs found

    ΠΠžΠ’Π«Π™ ΠΠΠ‘ΠžΠ  Π Π•ΠΠ“Π•ΠΠ’ΠžΠ’ Π”Π›Π― Π˜ΠœΠœΠ£ΠΠžΠ€Π•Π ΠœΠ•ΠΠ’ΠΠžΠ“Πž ΠžΠŸΠ Π•Π”Π•Π›Π•ΠΠ˜Π― Π—Π•ΠΠ ΠΠ›Π•ΠΠžΠΠ Π’ КОРМАΠ₯ И ΠŸΠ˜Π©Π•Π’Π«Π₯ ΠŸΠ ΠžΠ”Π£ΠšΠ’ΠΠ₯

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    The EIA-ZEARALENONE reagent kit for the determination of zearalenone mycotoxin in feed and food by direct ELISA using microtitration plate has been developed and tested. The evaluated technico-analytical parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of zearalenone content in agricultural products in a range of 50 to 800 mg/kg with proper accuracy and precision.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² ИЀА-Π—Π•ΠΠ ΠΠ›Π•ΠΠžΠ для опрСдСлСния микотоксина Π·Π΅Π°Ρ€Π°Π»Π΅Π½ΠΎΠ½Π° Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. УстановлСнныС Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‚ соврСмСнному ΡƒΡ€ΠΎΠ²Π½ΡŽ развития ΠΈΠΌΠΌΡƒΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ содСрТаниС Π·Π΅Π°Ρ€Π°Π»Π΅Π½ΠΎΠ½Π° Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ‚ 50 Π΄ΠΎ 800 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ

    РЕАГЕНВЫ Π”Π›Π― Π˜ΠœΠœΠ£ΠΠžΠ€Π•Π ΠœΠ•ΠΠ’ΠΠžΠ“Πž ΠžΠŸΠ Π•Π”Π•Π›Π•ΠΠ˜Π― ВОКБИНА Π’-2 Π’ КОРМАΠ₯ И ΠŸΠ˜Π©Π•Π’Π«Π₯ ΠŸΠ ΠžΠ”Π£ΠšΠ’ΠΠ₯

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    A reagent kit EIA-TOXIN T-2 for the determination of mycotoxin T-2 toxin in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of T-2 toxin content of agricultural products in a range of 30 to 1000 mg/kg with proper accuracy and precision.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² ИЀА-ВОКБИН Π’-2 для опрСдСлСния токсина Π’-2 Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. УстановлСнныС Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‚ соврСмСнному ΡƒΡ€ΠΎΠ²Π½ΡŽ развития ΠΈΠΌΠΌΡƒΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ содСрТаниС токсина Π’-2 Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ‚ 30 Π΄ΠΎ 1000 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ.Β 

    ΠΠžΠ’Π«Π™ ΠΠΠ‘ΠžΠ  Π Π•ΠΠ“Π•ΠΠ’ΠžΠ’ Π”Π›Π― ИММ Π£ΠΠžΠ€Π•Π ΠœΠ•ΠΠ’ΠΠžΠ“Πž ΠžΠŸΠ Π•Π”Π•Π›Π•ΠΠ˜Π― ΠΠ€Π›ΠΠ’ΠžΠšΠ‘Π˜ΠΠ Π’1 Π’ КОРМАΠ₯ И ΠŸΠ˜Π©Π•Π’Π«Π₯ ΠŸΠ ΠžΠ”Π£ΠšΠ’ΠΠ₯

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    A reagent kit EIA-AFLATOXIN for the determination of mycotoxin (aflatoxin B1) in feeds and foods by direct competitive enzyme immunoassay (EIA) has been developed and tested. The evaluated technico-analytical parameters of the kit and metrological characteristics of the measurement technique correspond to the modern level of EIA development and provide the determination of aflatoxin B1 content in agricultural products in the range from 2.0 to 50.0 mg/kg with proper accuracy and precision.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² ИЀА-ΠΠ€Π›ΠΠ’ΠžΠšΠ‘Π˜Π для опрСдСлСния микотоксина афлатоксина Π’1Β Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. УстановлСнныС Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‚ соврСмСнному ΡƒΡ€ΠΎΠ²Π½ΡŽ развития ΠΈΠΌΠΌΡƒΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ содСрТаниС афлатоксина Π’1Β Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ‚ 2,0 Π΄ΠΎ 50,0 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² ИЀА-ΠΠ€Π›ΠΠ’ΠžΠšΠ‘Π˜Π для опрСдСлСния микотоксина афлатоксина Π’1Β Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. УстановлСнныС Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‚ соврСмСнному ΡƒΡ€ΠΎΠ²Π½ΡŽ развития ΠΈΠΌΠΌΡƒΠ½ΠΎΠ°Π½Π°Π»ΠΈΠ·Π° ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ содСрТаниС афлатоксина Π’1Β Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ‚ 2,0 Π΄ΠΎ 50,0 ΠΌΠΊΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ

    Π˜ΠœΠœΠ£ΠΠžΠ€Π•Π ΠœΠ•ΠΠ’ΠΠΠ― Π‘Π˜Π‘Π’Π•ΠœΠ Π”Π›Π― ΠžΠŸΠ Π•Π”Π•Π›Π•ΠΠ˜Π― Π”Π•Π—ΠžΠšΠ‘Π˜ΠΠ˜Π’ΠΠ›Π•ΠΠžΠ›Π

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    A reagent kit EIA-DEOXYNIVALENOL for the determination of mycotoxin deoxynivalenol (DON) in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The basic components of the kit are polyclonal antibodies to DON, obtained as a result of immunization of rabbits with a conjugate of DON with bovine serum albumin and a conjugate of horseradish peroxidase with DON. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of DON content of agricultural products in a range of 0.2 to 6.0 mg/kg with proper accuracy and precision. The limit of quantitative determination of DON in grain and cereal foods does not exceed 0.2 mg/kg.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² «ИЀА-Π”Π•Π—ΠžΠšΠ‘Π˜ΠΠ˜Π’ΠΠ›Π•ΠΠžΠ›Β» для опрСдСлСния дСзоксинивалСнола (Π”ΠžΠ) Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… для ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…, ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΈ ΠΏΡ€ΠΎΠ΄ΠΎΠ²ΠΎΠ»ΡŒΡΡ‚Π²Π΅Π½Π½ΠΎΠΌ ΡΡ‹Ρ€ΡŒΠ΅ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. Π‘Π°Π·ΠΎΠ²Ρ‹Π΅ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½Ρ‚Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΏΡ€Π΅Π΄ΡΡ‚Π°Π²Π»ΡΡŽΡ‚ собой ΠΏΠΎΠ»ΠΈΠΊΠ»ΠΎΠ½Π°Π»ΡŒΠ½Ρ‹Π΅ Π°Π½Ρ‚ΠΈΡ‚Π΅Π»Π° ΠΊ Π”ΠžΠ, ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π½Ρ‹Π΅ Π² Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ ΠΈΠΌΠΌΡƒΠ½ΠΈΠ·Π°Ρ†ΠΈΠΈ ΠΊΡ€ΠΎΠ»ΠΈΠΊΠΎΠ² ΠΊΠΎΠ½ΡŠΡŽΠ³Π°Ρ‚ΠΎΠΌ Π”ΠžΠ с Π±Ρ‹Ρ‡ΡŒΠΈΠΌ сывороточным Π°Π»ΡŒΠ±ΡƒΠΌΠΈΠ½ΠΎΠΌ, ΠΈ ΠΊΠΎΠ½ΡŠΡŽΠ³Π°Ρ‚ пСроксидазы ΠΈΠ· ΠΊΠΎΡ€Π½Π΅ΠΉ Ρ…Ρ€Π΅Π½Π° с микотоксином. УстановлСны Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ. Набор позволяСт с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ Π”ΠžΠ Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΉ ΠΎΡ‚ 0,2 Π΄ΠΎ 6,0 ΠΌΠ³/ΠΊΠ³, ΠΏΡ€Π΅Π΄Π΅Π» количСствСнного опрСдСлСния исслСдуСмого микотоксина Π² Π·Π΅Ρ€Π½Π΅ ΠΈ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π°Ρ… Π΅Π³ΠΎ ΠΏΠ΅Ρ€Π΅Ρ€Π°Π±ΠΎΡ‚ΠΊΠΈ Π½Π΅ ΠΏΡ€Π΅Π²Ρ‹ΡˆΠ°Π΅Ρ‚ 0,2 ΠΌΠ³/ΠΊΠ³

    Π˜ΠœΠœΠ£ΠΠžΠ€Π•Π ΠœΠ•ΠΠ’ΠΠ«Π™ ΠΠΠΠ›Π˜Π— Π€Π£ΠœΠžΠΠ˜Π—Π˜ΠΠžΠ’ Π“Π Π£ΠŸΠŸΠ« Π’ Π’ КОРМАΠ₯ И ΠŸΠ˜Π©Π•Π’Π«Π₯ ΠŸΠ ΠžΠ”Π£ΠšΠ’ΠΠ₯

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    A reagent kit EIA-FUMONISIN for the determination of mycotoxins of fumonisin B group in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The evaluated technicoanalytical parameters of the kit and metrological characteristics of the technique of measurements correspond to the mo- dern level of immunoassay development and provide the determination of fumonisin group B content of agricultural products in a range of 0.11 to 6.0 mg/kg with proper accuracy and precision.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ ΠΏΡ€ΠΎΡˆΠ΅Π» Π²Π½ΡƒΡ‚Ρ€ΠΈΠ»Π°Π±ΠΎΡ€Π°Ρ‚ΠΎΡ€Π½Ρ‹Π΅ испытания Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² ИЀА-Π€Π£ΠœΠžΠΠ˜Π—Π˜Π для опрСдСлСния микотоксинов, относящихся ΠΊ Ρ„ΡƒΠΌΠΎΠ½ΠΈΠ·ΠΈΠ½Π°ΠΌ Π³Ρ€ΡƒΠΏΠΏΡ‹ Π’, Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° (ИЀА). Π’ состав Π½Π°Π±ΠΎΡ€Π° входят Ρ€Π°Π·Π±ΠΎΡ€Π½Ρ‹ΠΉ ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚, Π² Π»ΡƒΠ½ΠΊΠ°Ρ… ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠ³ΠΎ биоспСцифичСски ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·ΠΎΠ²Π°Π½ΠΎ моноклональноС Π°Π½Ρ‚ΠΈΡ‚Π΅Π»ΠΎ, Π³ΠΎΡ‚ΠΎΠ²Ρ‹ΠΉ ΠΊ использованию раствор ΠΊΠΎΠ½ΡŠΡŽΠ³Π°Ρ‚Π° Ρ„ΡƒΠΌΠΎΠ½ΠΈΠ·ΠΈΠ½Π° Π’1 с пСроксидазой ΠΈΠ· ΠΊΠΎΡ€Π½Π΅ΠΉ Ρ…Ρ€Π΅Π½Π°, Π³Ρ€Π°Π΄ΡƒΠΈΡ€ΠΎΠ²ΠΎΡ‡Π½Ρ‹Π΅ растворы, раствор Ρ…Ρ€ΠΎΠΌΠΎΠ³Π΅Π½Π° (Π’ΠœΠ‘), субстратный раствор (ΠΈΠ»ΠΈ Π³ΠΎΡ‚ΠΎΠ²Ρ‹ΠΉ ΠΊ использованию Ρ…Ρ€ΠΎΠΌΠΎΠ³Π΅Π½-субстратный раствор) ΠΈ стоп-Ρ€Π΅Π°Π³Π΅Π½Ρ‚. УстановлСнныС Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ ΡΠΎΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²ΡƒΡŽΡ‚ соврСмСнному ΡƒΡ€ΠΎΠ²Π½ΡŽ развития ИЀА ΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡŽΡ‚ с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ содСрТаниС Ρ„ΡƒΠΌΠΎΠ½ΠΈΠ·ΠΈΠ½ΠΎΠ² Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ‚ 0,11 Π΄ΠΎ 6,0 ΠΌΠ³/ΠΊΠ³ Π² ΡΠ΅Π»ΡŒΡΠΊΠΎΡ…ΠΎΠ·ΡΠΉΡΡ‚Π²Π΅Π½Π½ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ

    ΠœΠ΅Ρ‚ΠΎΠ΄ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° для опрСдСлСния охратоксина Π° Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²Ρ‹Ρ… ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π°Ρ…

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    A reagent kit EIA-OCHRATOXIN A for the determination of mycotoxin ochratoxin A in feeds and foods by aΒ  directΒ  competitiveΒ  enzymeΒ  immunoassayΒ  usingΒ  microtitrationΒ  plateΒ  hasΒ  beenΒ  developedΒ  andΒ  tested.Β  TheΒ  conjugate of ochratoxin A with horseradish peroxidase was synthesized using the mycotoxin aminoderivative joined to the carbohydrate chain of the enzyme. A monoclonal antibody to the mycotoxin was immobilized on the surface of plate wells through sheep anti-mouse antibodies. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of ochratoxin A content of agricultural products in a range of 5 to 375 mg/kg with proper accuracy and precision. The limit of quantitative determination of ochratoxin A in grain and cereal foods does not exceed 5 mg/kg.Π Π°Π·Ρ€Π°Π±ΠΎΡ‚Π°Π½ ΠΈ испытан Π½Π°Π±ΠΎΡ€ Ρ€Π΅Π°Π³Π΅Π½Ρ‚ΠΎΠ² «ИЀА-ОΠ₯РАВОКБИН А» для опрСдСлСния охратоксина А Π² ΠΊΠΎΡ€ΠΌΠ°Ρ… ΠΈ ΠΏΠΈΡ‰Π΅Π²ΠΎΠΉ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ прямого ΠΊΠΎΠ½ΠΊΡƒΡ€Π΅Π½Ρ‚Π½ΠΎΠ³ΠΎ ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° Π² ΠΌΠΈΠΊΡ€ΠΎΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π½ΠΎΠΌ Ρ„ΠΎΡ€ΠΌΠ°Ρ‚Π΅. ΠšΠΎΠ½ΡŠΡŽΠ³Π°Ρ‚ охратоксина А с пСроксидазой ΠΈΠ· ΠΊΠΎΡ€Π½Π΅ΠΉ Ρ…Ρ€Π΅Π½Π° ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½ с использованиСм Π°ΠΌΠΈΠ½ΠΎΠΏΡ€ΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΠΎΠ³ΠΎ микотоксина, присоСдинСнного ΠΊ ΡƒΠ³Π»Π΅Π²ΠΎΠ΄Π½ΠΎΠΉ Ρ†Π΅ΠΏΠΈ Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚Π°. МоноклональноС Π°Π½Ρ‚ΠΈΡ‚Π΅Π»ΠΎ ΠΊ опрСдСляСмому микотоксину ΠΈΠΌΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·ΠΎΠ²Π°Π½ΠΎ Π² Π»ΡƒΠ½ΠΊΠ°Ρ… ΠΏΠ»Π°Π½ΡˆΠ΅Ρ‚Π° Ρ‡Π΅Ρ€Π΅Π· Π°Π½Ρ‚ΠΈΠ²ΠΈΠ΄ΠΎΠ²Ρ‹Π΅ Π°Π½Ρ‚ΠΈΡ‚Π΅Π»Π° Π±Π°Ρ€Π°Π½Π° ΠΊ ΠΈΠΌΠΌΡƒΠ½ΠΎΠ³Π»ΠΎΠ±ΡƒΠ»ΠΈΠ½Π°ΠΌ ΠΌΡ‹ΡˆΠΈ. УстановлСны Ρ‚Π΅Ρ…Π½ΠΈΠΊΠΎ-аналитичСскиС ΠΏΠ°Ρ€Π°ΠΌΠ΅Ρ‚Ρ€Ρ‹ Π½Π°Π±ΠΎΡ€Π° «ИЀА-ОΠ₯РАВОКБИН А» ΠΈ мСтрологичСскиС характСристики ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ выполнСния ΠΈΠ·ΠΌΠ΅Ρ€Π΅Π½ΠΈΠΉ. Набор позволяСт с Π½Π°Π΄Π»Π΅ΠΆΠ°Ρ‰Π΅ΠΉ Ρ‚ΠΎΡ‡Π½ΠΎΡΡ‚ΡŒΡŽ ΠΎΠΏΡ€Π΅Π΄Π΅Π»ΡΡ‚ΡŒ охратоксин А Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ содСрТания ΠΎΡ‚ 5 Π΄ΠΎ 375 ΠΌΠΊΠ³/ΠΊΠ³, ΠΏΡ€Π΅Π΄Π΅Π» количСствСнного опрСдСлСния микотоксина Π² Π·Π΅Ρ€Π½Π΅ ΠΈ Π² ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π°Ρ… Π΅Π³ΠΎ ΠΏΠ΅Ρ€Π΅Ρ€Π°Π±ΠΎΡ‚ΠΊΠΈ составляСт 5 ΠΌΠΊΠ³/ΠΊΠ³

    Diagnostics turbocompressors of diesels engine at running using repair and restoration compositions

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    The article dwells upon the importance of increasing reliability of turbocompressors of internal combustion engines. There is a suggestion on causes of increased wear of the unit’s bearings. There are set the problems of improving the wear resistance of the unit using repair and restoration compositions (RRC) at the stage of the running. To assess the quality of repair and to determine the completion’s moment of processing RRC unit the use of vibro-acoustic method of diagnostics is justified

    Β«Ecological EconomyΒ»: The Most Important Aspect of the Ideology of the Global Commons in Supporting Sustainable Socio-Economic Development

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    Aim. To study the methodological foundations and main provisions of a new vector of economic development, the "ecological economy", as being the most important aspect of the Global Commons ideology.Material and Methods. The study was conducted using a range of theoretical research methods, including analysis, deduction and forecasting.Discussion. The basis of the Global Commons ideology is considered to be the "green economy", a new technological modality, the purpose of which is the careful and rational utilisation of available resources employing high technologies for the maximum satisfaction of people's needs. Proponents of the Global Commons ideology advocate the ecology or the environment as the dominant factors in sustainable socio-economic development. At the same time, the environmental policy of the developed world also represents a new form of neocolonialism directed at the takeover of strategic natural resources of underdeveloped countries, while restricting their rights to technological and social development. It may even be aimed at the military or economic capture of national natural and biological resources. All this challenges individual state sovereignty and may lead to a fundamentally new type of conflict in the future.Conclusions. The formation of the "ecological economy" is a new vector of sustainable socio-economic development in the Global Commons ideology, requiring our governmental authorities to take the necessary organizational, military, managerial and regulatory measures to realize the nation's competitive advantages, to conserve its natural potential, to block non-residents from capturing national wealth and to organise an effective system of environmental security
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