Uso del taping en el manejo clínico de sujetos con pinzamiento sub-acromial: Revisión sistemática

The sub-acromial impingement (SAI) is the most common musculoskeletal disorder of shoulder pathologies. In recent times, the taping has become a relatively new and quite common indication in recent years. There will be a synthesis of evidence through a systematic review (SR) of randomized trials that had compared the use of taping for the management of these patients. The aim of this review is to determine the effect of taping on pain in subjects with sub-acromial pinch. The research strategy included randomized clinical trials (RCTs) databases were used: Medline, Cinahl, PEDro, Central, LILACS. In addition, we searched electronic journals, conference proceedings, randomized trials registry and contacted experts in the field. The results were two articles that met our eligibility criteria. We conclude that there is low quality evidence suggesting that the application of taping, would be effective to improve functionality and reduce pain at rest and during movements up to 1 week after application, in subjects with sub-acromial pinch compared to physical therapy program.El pinzamiento sub-acromial (SPS) es el trastorno músculo-esquelético más frecuente de las patologías de hombro. El taping se ha transformado en una indicación relativamente nueva y bastante común en los últimos años. Se realizará una síntesis de la evidencia a través de una revisión sistemática (RS) de ensayos clínicos aleatorizados que hayan comparado el uso del taping para el manejo de estos pacientes. El objetivo de esta revisión es determinar el efecto del taping sobre el dolor en sujetos con pellizcamiento sub-acromial. La estrategia de búsqueda incluyó ensayos clínicos aleatorizados (ECA); las bases de datos usadas fueron: Medline, Cinahl, PEDro, Central, LILACS. Además, se realizó una búsqueda en revistas electrónicas, actas de congresos, Registro de Estudios Clínicos Aleatorizados y se contactó con expertos en el tema. Los resultados obtenidos fueron de dos artículos que cumplían con nuestros criterios de elegibilidad. Se concluye que existe evidencia de baja calidad que sugiere que la aplicación de taping sería efectiva para mejorar la funcionalidad, reducir el dolor en reposo y durante los movimientos hasta en una semana posterior a su aplicación, en sujetos que presentan pellizcamiento sub-acromial en comparación a un programa de terapia física

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

International audienceIn 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field