Aerosol mass and black carbon concentrations, a two year record at NCO-P (5079 m, Southern Himalayas)

Aerosol mass and the absorbing fraction are important variables, needed to constrain the role of atmospheric particles in the Earth radiation budget, both directly and indirectly through CCN activation. In particular, their monitoring in remote areas and mountain sites is essential for determining source regions, elucidating the mechanisms of long range transport of anthropogenic pollutants, and validating regional and global models. Since March 2006, aerosol mass and black carbon concentration have been monitored at the Nepal Climate Observatory-Pyramid, a permanent high-altitude research station located in the Khumbu valley at 5079 m a.s.l. below Mt. Everest. The first two-year averages of PM<sub>1</sub> and PM<sub>1−10</sub> mass were 1.94 μg m<sup>−3</sup> and 1.88 μg m<sup>−3</sup>, with standard deviations of 3.90 μg m<sup>−3</sup> and 4.45 μg m<sup>−3</sup>, respectively, while the black carbon concentration average is 160.5 ng m<sup>−3</sup>, with a standard deviation of 296.1 ng m<sup>−3</sup>. Both aerosol mass and black carbon show well defined annual cycles, with a maximum during the pre-monsoon season and a minimum during the monsoon. They also display a typical diurnal cycle during all the seasons, with the lowest particle concentration recorded during the night, and a considerable increase during the afternoon, revealing the major role played by thermal winds in influencing the behaviour of atmospheric compounds over the high Himalayas. The aerosol concentration is subject to high variability: in fact, as well as frequent "background conditions" (55% of the time) when BC concentrations are mainly below 100 ng m<sup>−3</sup>, concentrations up to 5 μg m<sup>−3</sup> are reached during some episodes (a few days every year) in the pre-monsoon seasons. The variability of PM and BC is the result of both short-term changes due to thermal wind development in the valley, and long-range transport/synoptic circulation. At NCO-P, higher concentrations of PM<sub>1</sub> and BC are mostly associated with regional circulation and westerly air masses from the Middle East, while the strongest contributions of mineral dust arrive from the Middle East and regional circulation, with a special contribution from North Africa and South-West Arabian Peninsula in post-monsoon and winter season

Analysis of Summer Ozone Observations at a High Mountain Site in Central Italy (Campo Imperatore, 2388 m a.s.l.)

Tropospheric ozone (O3) is an important atmospheric pollutant and climate forcer. The Mediterranean basin is a hot-spot region in terms of short-term O3 distribution, with frequent episodes of high tropospheric O3, especially during summer. To improve the characterisation of summer O3 variability in the Mediterranean area, during the period 6–27 August 2009 an experimental campaign was conducted at Campo Imperatore, Mt Portella (CMP), a high mountain site (2,388 m a.s.l.) located in the central Italian Apennines. As deduced from analysis of atmospheric circulation, the measurement site was significantly affected by air masses originating over the Mediterranean basin, which affected the measurement site for 32 % of the time. Analysis of average values and diurnal and day-to-day variability revealed that CMP O3 observations (average value 60.0 ± 5.1 ppbv) were comparable with measurements at other European mountain stations, indicating a prevalent effect of meteorological conditions and atmospheric transport on the synoptic scale. In fact, only a small "reverse" diurnal variation typically characterises diurnal O3 variability because of local thermal wind circulation, which sporadically favours transport of air masses rich in O3 from the foothill regions. Statistical analysis of five-day back-trajectory ensembles indicates that synoptic-scale air-mass transport from the Mediterranean Sea usually results in decreasing O3 concentrations at CMP, whereas the highest hourly O3 values are mostly associated with air masses from central continental Europe, eastern Europe, and northern Italy. High O3 concentrations are also related to downward air-mass transport from higher altitudes. Comparison of in-situ O3 variability with tropospheric O3 satellite-based measurements reveals similar features of the two data sets. Together with the results from back-trajectory analysis, this indicates that CMP measurements might usefully improve characterisation of broad-scale O3 variability over the central Mediterranean basin

liquid biopsy as tool to monitor and predict clinical benefit from chemotherapy ct and immunotherapy it in advanced non small cell lung cancer ansclc a prospective study

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Determination of galangin in commercial extracts of Alpinia officinarum by RP-HPLC-DAD

Alpinia officinarum has been used by long time in the traditional medicine and several commercial extracts containing it are available on the market. In this paper a reversed phase high performance liquid chromatography-diode array detection (RP-HPLC-DAD) method for Alpinia officinarum preparations was proposed using galangin as a marker. The quantification was undertaken by a combination of RP-HPLC-DAD. The LC system employed a Gemini Phenomenex RP-18 column with phosphoric acid, tetrahydrofuran and acetonitrile as mobile phase and detection at 267 nm. The method validation was throughout satisfactory. The determination coefficient was r2 = 0.998 with LOD 0.48 µg mL-1 and LOQ 1.59 µg mL-1 The results of precision (RSD < 2.82) and accuracy (99.9 %, 100.4 %, 102.2 %) with . RSD less than 2.7 %, were satisfactory. The analytical method was applied to commercial extracts, and was suitable to provide qualitative and quantitative information for the quality control to commercial preparations.Colegio de Farmacéuticos de la Provincia de Buenos Aire

The Zea mays mutants opaque-2 and opaque-7 disclose extensive changes in endosperm metabolism as revealed by protein, amino acid, and transcriptome-wide analyses

<p>Abstract</p> <p>Background</p> <p>The changes in storage reserve accumulation during maize (<it>Zea mays </it>L.) grain maturation are well established. However, the key molecular determinants controlling carbon flux to the grain and the partitioning of carbon to starch and protein are more elusive. The <it>Opaque-2 </it>(<it>O2</it>) gene, one of the best-characterized plant transcription factors, is a good example of the integration of carbohydrate, amino acid and storage protein metabolisms in maize endosperm development. Evidence also indicates that the <it>Opaque-7 </it>(<it>O7</it>) gene plays a role in affecting endosperm metabolism. The focus of this study was to assess the changes induced by the <it>o2 </it>and <it>o7 </it>mutations on maize endosperm metabolism by evaluating protein and amino acid composition and by transcriptome profiling, in order to investigate the functional interplay between these two genes in single and double mutants.</p> <p>Results</p> <p>We show that the overall amino acid composition of the mutants analyzed appeared similar. Each mutant had a high Lys and reduced Glx and Leu content with respect to wild type. Gene expression profiling, based on a unigene set composed of 7,250 ESTs, allowed us to identify a series of mutant-related down (17.1%) and up-regulated (3.2%) transcripts. Several differentially expressed ESTs homologous to genes encoding enzymes involved in amino acid synthesis, carbon metabolism (TCA cycle and glycolysis), in storage protein and starch metabolism, in gene transcription and translation processes, in signal transduction, and in protein, fatty acid, and lipid synthesis were identified. Our analyses demonstrate that the mutants investigated are pleiotropic and play a critical role in several endosperm-related metabolic processes. Pleiotropic effects were less evident in the <it>o7 </it>mutant, but severe in the <it>o2 </it>and <it>o2o7 </it>backgrounds, with large changes in gene expression patterns, affecting a broad range of kernel-expressed genes.</p> <p>Conclusion</p> <p>Although, by necessity, this paper is descriptive and more work is required to define gene functions and dissect the complex regulation of gene expression, the genes isolated and characterized to date give us an intriguing insight into the mechanisms underlying endosperm metabolism.</p

Euclid: Forecasts from redshift-space distortions and the Alcock-Paczynski test with cosmic voids

Euclid is poised to survey galaxies across a cosmological volume of unprecedented size, providing observations of more than a billion objects distributed over a third of the full sky. Approximately 20 million of these galaxies will have their spectroscopy available, allowing us to map the three-dimensional large-scale structure of the Universe in great detail. This paper investigates prospects for the detection of cosmic voids therein and the unique benefit they provide for cosmological studies. In particular, we study the imprints of dynamic (redshift-space) and geometric (Alcock-Paczynski) distortions of average void shapes and their constraining power on the growth of structure and cosmological distance ratios. To this end, we made use of the Flagship mock catalog, a state-of-the-art simulation of the data expected to be observed with Euclid. We arranged the data into four adjacent redshift bins, each of which contains about 11000 voids and we estimated the stacked void-galaxy cross-correlation function in every bin. Fitting a linear-theory model to the data, we obtained constraints on f/b and DMH, where f is the linear growth rate of density fluctuations, b the galaxy bias, D-M the comoving angular diameter distance, and H the Hubble rate. In addition, we marginalized over two nuisance parameters included in our model to account for unknown systematic effects in the analysis. With this approach, Euclid will be able to reach a relative precision of about 4% on measurements of f/b and 0.5% on DMH in each redshift bin. Better modeling or calibration of the nuisance parameters may further increase this precision to 1% and 0.4%, respectively. Our results show that the exploitation of cosmic voids in Euclid will provide competitive constraints on cosmology even as a stand-alone probe. For example, the equation-of-state parameter, w, for dark energy will be measured with a precision of about 10%, consistent with previous more approximate forecasts

Euclid: Cosmological forecasts from the void size function

The Euclid mission $-$ with its spectroscopic galaxy survey covering a sky area over $15\,000 \ \mathrm{deg}^2$ in the redshift range $0.9<1.8\ -$ will provide a sample of tens of thousands of cosmic voids. This paper explores for the first time the constraining power of the void size function on the properties of dark energy (DE) from a survey mock catalogue, the official Euclid Flagship simulation. We identify voids in the Flagship light-cone, which closely matches the features of the upcoming Euclid spectroscopic data set. We model the void size function considering a state-of-the art methodology: we rely on the volume conserving (Vdn) model, a modification of the popular Sheth & van de Weygaert model for void number counts, extended by means of a linear function of the large-scale galaxy bias. We find an excellent agreement between model predictions and measured mock void number counts. We compute updated forecasts for the Euclid mission on DE from the void size function and provide reliable void number estimates to serve as a basis for further forecasts of cosmological applications using voids. We analyse two different cosmological models for DE: the first described by a constant DE equation of state parameter, $w$, and the second by a dynamic equation of state with coefficients $w_0$ and $w_a$. We forecast $1\sigma$ errors on $w$ lower than the $10\%$, and we estimate an expected figure of merit (FoM) for the dynamical DE scenario $\mathrm{FoM}_{w_0,w_a} = 17$ when considering only the neutrino mass as additional free parameter of the model. The analysis is based on conservative assumptions to ensure full robustness, and is a pathfinder for future enhancements of the technique. Our results showcase the impressive constraining power of the void size function from the Euclid spectroscopic sample, both as a stand-alone probe, and to be combined with other Euclid cosmological probes...