Translation arrest of potato virus X RNA in Krebs-2 cell-free system: RNase H cleavage promoted by complementary oligodeoxynucleotides

AbstractTranslation arrest of genomic potato virus X (PVX) RNA promoted by complementary oligodeoxynucleotides in Krebs-2 cell-free system is described. 14–15 mer oligodeoxynucleotides complementary to the 5′-proximal cistron of PVX RNA were shown to induce specific truncation of the major non-structural polypeptide coded by PVX RNA. Evidence is presented that effective translational arrest of PVX RNA in the presence of complementary oligonucleotides restults from the site-specific cleavage of RNA by endogenous RNase H intrinsic to the Krebs-2 extract. No similar translational arrest was found in the rabbit reticulocyte lysate cell-free system

Isospin Splitting in the Baryon Octet and Decuplet

Baryon mass splittings are analyzed in terms of a simple model with general pairwise interactions. At present, the $\Delta$ masses are poorly known from experiments. Improvement of these data would provide an opportunity to make a significant test of our understanding of electromagnetic and quark-mass contributions to hadronic masses. The problem of determining resonance masses from scattering and production data is discussed.Comment: 9 pages, LATEX inc. 2 LATEX "pictures", CMU-HEP91-24-R9

Nonlinear parameters of surface EMG in schizophrenia patients depend on kind of antipsychotic therapy

ArticleWe compared a set of surface EMG (sEMG) parameters in several groups of schizophrenia (SZ, n = 74) patients and healthy controls (n = 11) and coupled them with the clinical data. sEMG records were quantified with spectral, mutual information (MI) based and recurrence quantification analysis (RQA) parameters, and with approximate and sample entropies (ApEn and SampEn). Psychotic deterioration was estimated with Positive and Negative Syndrome Scale (PANSS) and with the positive subscale of PANSS. Neuroleptic-induced parkinsonism (NIP) motor symptoms were estimated with Simpson-Angus Scale (SAS). Dyskinesia was measured with Abnormal Involuntary Movement Scale (AIMS). We found that there was no difference in values of sEMG parameters between healthy controls and drug-naïve SZ patients. The most specific group was formed of SZ patients who were administered both typical and atypical antipsychotics (AP). Their sEMG parameters were significantly different from those of SZ patients taking either typical or atypical AP or taking no AP. This may represent a kind of synergistic effect of these two classes of AP. For the clinical data we found that PANSS, SAS, and AIMS were not correlated to any of the sEMG parameters. Conclusion: with nonlinear parameters of sEMG it is possible to reveal NIP in SZ patients, and it may help to discriminate between different clinical groups of SZ patients. Combined typical and atypical AP therapy has stronger effect on sEMG than a therapy with AP of only one class.Publisher's pdfhttp://purl.org/eprint/status/PeerReviewe

Photometric observations of the radio bright B[e]/X-ray binary CI Cam

We present multiwavelength (optical, IR, radio) observations of CI Cam, the optical counterpart to the transient X-ray source XTE J0421+560. Pre-outburst quiescent observations reveal the presence of a dusty envelope around the system. Pronounced short term variability is observed at all wavebands from U-K, but no indication of prior flaring of a similar magnitude to the 1998 April outburst is found in these data. Data obtained during the 1998 April X-ray flare reveal pronounced optical-radio flaring. The optical flux was observed to quickly return to quiescent levels, while the radio flare was of much longer duration. The optical component is likely to result from a combination of free-free/free-bound emission, emission line and thermal dust emission, caused by re-radiation of the X-ray flux, while the behaviour of the multiwavelength radio data is consistent with emission from expanding ejecta emitting via the synchrotron mechanism. Post-outburst (1998 August-1999 March) U-M broadband photometric observations reveal that while the optical (UBV) flux remains at pre-outburst quiescent levels, near IR (JHKLM) fluxes exceed the pre-outburst fluxes by ~0.5 magnitudes. Modelling the pre- and post-outburst spectral energy distribution of CI Cam reveals that the structure and/or composition of the dusty component of the circumstellar envelope appears to have changed. Due to a lack of information on the precise chemical composition of the dust within the system several explanations for this behaviour are possible, such as the production of new dust at the inner edge of the envelope, or modification of the composition of the dust due to X-ray irradiation

Broken symmetries and directed collective energy transport

We study the appearance of directed energy current in homogeneous spatially extended systems coupled to a heat bath in the presence of an external ac field E(t). The systems are described by nonlinear field equations. By making use of a symmetry analysis we predict the right choice of E(t) and obtain directed energy transport for systems with a nonzero topological charge Q. We demonstrate that the symmetry properties of motion of topological solitons (kinks and antikinks) are equivalent to the ones for the energy current. Numerical simulations confirm the predictions of the symmetry analysis and, moreover, show that the directed energy current drastically increases as the dissipation parameter $\alpha$ reduces. Our results generalize recent rigorous theories of currents generated by broken time-space symmetries to the case of interacting many-particle systems.Comment: 4 pages, 2 figure

Breathers in Josephson junction ladders: resonances and electromagnetic waves spectroscopy

We present a theoretical study of the resonant interaction between dynamical localized states (discrete breathers) and linear electromagnetic excitations (EEs) in Josephson junction ladders. By making use of direct numerical simulations we find that such an interaction manifests itself by resonant steps and various sharp switchings (voltage jumps) in the current-voltage characteristics. Moreover, the power of ac oscillations away from the breather center (the breather tail) displays singularities as the externally applied dc bias decreases. All these features can be mapped to the spectrum of EEs that has been derived analytically and numerically. Using an improved analysis of the breather tail, a spectroscopy of the EEs is developed. The nature of breather instability driven by localized EEs is established.Comment: 15 pages, 13 figure