Study of the UV Irradiation and Nalidicsic Acid Effect on the RecA-protein Induction in <i>Francisella tularensis</i> 15/10 Cells

Studied is the UV irradiation and nalidicsic acid effect on the RecA-protein synthesis in Francisella tularensis 15/10 cells. Obtained is the specific murine serum to the recombinant RecA-protein. The results of immunoblotting with this specific serum demonstrate that the amount of RecA-protein in Francisella tularensis 15/10 cells is not increased after the exposure to these damaging factors

Immunobiological Properties of <i>Francisella tularensis</i> 15/10 Strain with Deleted recA Gene

Deletion of recA gene in Francisella tularensis 15/10 genome leads to the increase in its sensitivity to ultraviolet irradiation, reduction of the homologous recombination capacity, and a slight decline of virulence for mice. Efficacy of Francisella tularensis 15/10ΔrecA reproduction within microphage-like cells, its resistance to normal rabbit serum, and protective properties on the mouse model of tularemia are the same as in original Francisella tularensis 15/10

Construction and Investigation of the Vaccine Strain Francisella tularensis without iglC genes. Communication 1

Using site-specific mutagenesis constructed were the variants of the vaccine strain F. tularensis subsp. holarctica 15 with the deleted iglC genes. Identified was the fact that genome of the strain 15 contained two copies of iglC gene. Deletion of one of them as well as both had little effect on the cultural-morphological and growth properties of the microbe. At the same time F. tularensis 15 lacking one copy of the iglC gene propagated in mice macrophages several times slower, than the original strain. Inactivation of both of the copies in the chromosome leaded to the emergence of a variant incapable of intracellular reproduction. This capacity in F. tularensis 15/23-2 with two inactivated iglC gene copies was partially recovered after integration of a complementing plasmid. Therewith the data mentioned above testifies to the significance of iglC gene for the process of reproduction in macrophages

Construction and Investigation of the Variants of the Vaccine Strain <I>Francisella tularensis</I> Lacking <I>iglC </I>Genes. Communication 2

for BALB/c mice by an order while retaining protective properties. Strain Francisella tularensis 15 lacking two copies of the iglC gene cannot replicate in mice organism, induces weak humoral response, is fully avirulent, and has decreased protective activity. Treatment of mice with sera obtained from the animals immunized on day 49 post immunization both with the stock F. tularensis 15 strain and its variants with one or two inactivated iglC genes has provided for 100 % protection from challenge with 200 DCL of F. tularensis 15 strain. However in case of BALB/c mice exposure to virulent F. tularensis 503 and F. tularensis Schu strains (50 DCL and 25 DSL, respectively), treated with immune sera 24 hours before, registered has been only mean lifetime increase

Isolation of Central Asian Subspecies of Tularemia Agent in the Altai Territory

Within the frames of activities attributed to the Reference Center for tularemia monitoring at SRC AMB, genetically identified are 4 isolates of Francisella tularensis , isolated in 2011 in the Altai Territory. These bacteria prove to be virulent for BALB/c mice, DCL being lower than 10 CFU. Using single-primer PCR-typing and MLVA assay distinguished have been the subspecies of the isolates. Three of them refer to the Central Asian subspecies, one – to the Holarctic, the former being isolated in the territory of the Russian Federation for the first time ever

Орхидные России : (биология, экология и охрана) [Orchids of Russia : (biology, ecology and protection)]

В книге изложены результаты более чем тридцатилетнего изучения дикорастущих орхидных (Orchidaceae) в различных регионах России (от Калининградской области до Дальнего Востока, от Кольского п-ова до Кавказа) и за её пределами. Исследованы различные аспекты жизни орхидных — морфология и онтогенез, экология и фитоценология, микоризообразование, сезонный ритм, способы и интенсивность размножения, возрастная структура, устойчивость и динамика популяций, реакция на различные формы антропогенного воздействия, состояние их охраны. Обобщён большой объём отечественной и иностранной литературы. Полученные результаты легли в основу характеристик свыше 125 видов семейства Orchidaceae, отмеченных на территории России. Приведены карты ареалов и цветные изображения каждого вида. - Книга может быть полезна ученым-ботаникам, преподавателям высших учебных заведений и использована при разработке практических мер по сохранению видов этого семейства. The book presents the results of a long-term (over 30 years) study of the family Orchidaceae across Russia: from Kaliningrad Province to the Far East and from the Cola Peninsula to the Caucasus, as well as in surrounding countries. Along with an insight into a history of orchid research in Russia, the book provides a review of modern knowledge in orchid morphology, habitats and seasonal development, and reproductive strategies, mycorrhiza, pollination and species geographic range and conservation status in Russia. The book includes descriptions of more than 125 orchid species illustrated with colour images and maps

PCR Differentiation of <i>Francisella tularensis</i> Subspecies Using One Primer

Presented are experimental data of size distribution of amplicons obtained with the help of the primer consisting of Escherichia coli chi-sequence and DNA from different subspecies of Francisella tularensis strains. Analysis of this distribution permitted to determine five informative regions on the electrophoregrams (in the regions of 190, 280, 500-570, 830 and 950 b.p.), that made it possible to perform subspecies differentiation of tularemia microbe strains. Thus, the subspecies of F. tularensis clinical isolates can be identified by safe, fast and convenient method - the PCR analysis using only one primer Chi 1f despite the slight genetic heterogeneity of tularemia agent strains