Probing protein-protein interactions by dynamic force correlated spectroscopy (FCS)

We develop a formalism for single molecule dynamic force spectroscopy to map the energy landscape of protein-protein complex ($P_1$$P_2$). The joint distribution $P(\tau_1,\tau_2)$ of unbinding lifetimes $\tau_1$ and $\tau_2$ measurable in a compression-tension cycle, which accounts for the internal relaxation dynamics of the proteins under tension, shows that the histogram of $\tau_1$ is not Poissonian. The theory is applied to the forced unbinding of protein $P_1$, modeled as a wormlike chain, from $P_1$$P_2$. We propose a new class of experiments which can resolve the effect of internal protein dynamics on the unbinding lifetimes.Comment: 12 pages, 3 figures, accepted to Phys. Rev. Let

Fluctuations, Higher Order Anharmonicities, and Landau Expansion for Barium Titanate

Correct phenomenological description of ferroelectric phase transitions in barium titanate requires accounting for eighth-order terms in the free energy expansion, in addition to the conventional sixth-order contributions. Another unusual feature of BaTiO_3 crystal is that the coefficients B_1 and B_2 of the terms P_x^4 and P_x^2*P_y^2 in the Landau expansion depend on the temperature. It is shown that the temperature dependence of B_1 and B_2 may be caused by thermal fluctuations of the polarization, provided the fourth-order anharmonicity is anomalously small, i. e. the nonlinearity of P^4 type and higher-order ones play comparable roles. Non-singular (non-critical) fluctuation contributions to B_1 and B_2 are calculated in the first approximation in sixth-order and eighth-order anharmonic constants. Both contributions increase with the temperature, which is in agreement with available experimental data. Moreover, the theory makes it possible to estimate, without any additional assumptions, the ratio of fluctuation (temperature dependent) contributions to coefficients B_1 and B_2. Theoretical value of B_1/B_2 appears to be close to that given by experiments.Comment: 5 pages, 1 figur

Disentangling Morphology, Star Formation, Stellar Mass, and Environment in Galaxy Evolution

We present a study of the spectroscopic and photometric properties of galaxies in six nearby clusters. We perform a partial correlation analysis on our dataset to investigate whether the correlation between star formation rates in galaxies and their environment is merely another aspect of correlations of morphology, stellar mass, or mean stellar age with environment, or whether star formation rates vary independently of these other correlations. We find a residual correlation of ongoing star formation with environment, indicating that even galaxies with similar morphologies, stellar masses, and mean stellar ages have lower star formation rates in denser environments. Thus, the current star formation gradient in clusters is not just another aspect of the morphology-density, stellar mass-density, or mean stellar age-density relations. Furthermore, the star formation gradient cannot be solely the result of initial conditions, but must partly be due to subsequent evolution through a mechanism (or mechanisms) sensitive to environment. Our results constitute a true ``smoking gun'' pointing to the effect of environment on the later evolution of galaxies.Comment: 31 pages, including 5 figures; accepted for publication in Ap

London Congestion Charging: Successes, Gaps and Future Opportunities Offered by Cooperative ITS

The London Congestion Charging (LCC) scheme was initially introduced on 17 February 2003. Being the largest of its kind and employing advanced technology, it marked a major innovation in the field of urban road user charging and provided inspiration to several other cities worldwide. Nine years on, and following a number of operational changes that have taken place, this study analyzes successes and pitfalls, and identifies potential future opportunities in the light of latest technological developments in the field of cooperative Intelligent Transport Systems (ITS). The analysis concentrates primarily on the LCC scheme itself, but draws broader conclusions about the future of urban road charging in general

Condensation transition in DNA-polyaminoamide dendrimer fibers studied using optical tweezers

When mixed together, DNA and polyaminoamide (PAMAM) dendrimers form fibers that condense into a compact structure. We use optical tweezers to pull condensed fibers and investigate the decondensation transition by measuring force-extension curves (FECs). A characteristic plateau force (around 10 pN) and hysteresis between the pulling and relaxation cycles are observed for different dendrimer sizes, indicating the existence of a first-order transition between two phases (condensed and extended) of the fiber. The fact that we can reproduce the same FECs in the absence of additional dendrimers in the buffer medium indicates that dendrimers remain irreversibly bound to the DNA backbone. Upon salt variation FECs change noticeably confirming that electrostatic forces drive the condensation transition. Finally, we propose a simple model for the decondensing transition that qualitatively reproduces the FECs and which is confirmed by AFM images.Comment: Latex version, 4 pages+3 color figure

Absolute Proper Motion of the Fornax Dwarf Spheroidal Galaxy from Photographic and HST WFPC2 Data

We have measured the absolute proper motion of the Fornax dwarf spheroidal galaxy from a combination of photographic plate material and HST WFPC2 data that provide a time baseline of up to 50 years. The extragalactic reference frame consists of 8 QSO images and 48 galaxies. The absolute proper motion is mu_alpha cos(delta) = 0.59 +-0.16 mas/yr and mu_delta = -0.15 +- 0.16 mas/yr. The corresponding orbit of Fornax is polar, with an eccentricity of 0.27, and a radial period of 4.5 Gyr. Fornax's current location is near pericenter. The direction of the motion of Fornax supports the notion that Fornax belongs to the Fornax-LeoI-LeoII-Sculptor-Sextans stream as hypothesized by Lynden-Bell (1976, 1982) and Majewski (1994). According to our orbit determination, Fornax crossed the Magellanic plane \~190 Myr ago, a time that coincides with the termination of the star-formation process in Fornax. We propose that ram-pressure stripping due to the passage of Fornax through a gaseous medium denser than the typical intragalactic medium left behind from the LMC may have caused the end of star formation in Fornax. The excess, anomalous clouds within the South Galactic pole region of the Magellanic Stream whose origin has long been debated in the literature as constituents of either the Magellanic Stream or of the extragalactic Sculptor group, are found to lie along the orbit of Fornax. We speculate that these clouds are stripped material from Fornax as the dwarf crossed the Magellanic Clouds' orbit.Comment: Accepted for publication in Astronomical Journal. The version with high resolution figures can be found at ftp://pegasus.astro.yale.edu/pub/dana/paper

Local Hidden Variable Theories for Quantum States

While all bipartite pure entangled states violate some Bell inequality, the relationship between entanglement and non-locality for mixed quantum states is not well understood. We introduce a simple and efficient algorithmic approach for the problem of constructing local hidden variable theories for quantum states. The method is based on constructing a so-called symmetric quasi-extension of the quantum state that gives rise to a local hidden variable model with a certain number of settings for the observers Alice and Bob.Comment: 8 pages Revtex; v2 contains substantial changes, a strengthened main theorem and more reference

Critical Current Oscillations in Strong Ferromagnetic Pi-Junctions

We report magnetic and electrical measurements of Nb Josephson junctions with strongly ferromagnetic barriers of Co, Ni and Ni80Fe20 (Py). All these materials show multiple oscillations of critical current with barrier thickness implying repeated 0-pi phase-transitions in the superconducting order parameter. We show in particular that the Co barrier devices can be accurately modelled using existing clean limit theories and so that, despite the high exchange energy (309 meV), the large IcRN value in the pi-state means Co barriers are ideally suited to the practical development of superconducting pi-shift devices.Comment: 4 pages 3 figures 1 table. Revised version as accepted for publication. To appear in Physical Review Letter