Comparison, Inclusiveness, and Non-Hierarchical Incommensurability: Narrative Strategies in Two New Aboriginal Life Stories

Originally published in Biomedical Optics Express on 01 July 2015 (boe-6-7-2512

Quantum Dots in an Amphiphilic Polyethyleneimine Derivative Platform for Cellular Labeling, Targeting, Gene Delivery, and Ratiometric Oxygen Sensing

Amphiphilic polyethyleneimine derivatives (amPEIs) were synthesized and used to encapsulate dozens of quantum dots (QDs). The QD–amPEI composite was ∼100 nm in hydrodynamic diameter and had the slightly positive outer surface that suited well for cellular internalization. The QD–amPEI showed very efficient QD cellular labeling with the labeled cell fluorescence intensity more than 10 times higher than conventional techniques such as Lipofectamine-assisted QD delivery. QD–amPEI was optimal for maximal intracellular QD delivery by the large QD payload and the rapid endocytosis kinetics. QD–amPEI platform technology was demonstrated for gene delivery, cell-specific labeling, and ratiometric oxygen sensing. Our QD–amPEI platform has two partitions: positive outer surface and hydrophobic inside pocket. The outer positive surface was further exploited for gene delivery and targeting. Co-delivery of QDs and GFP silencing RNAs was successfully demonstrated by assembling siRNAs to the outer surfaces, which showed the transfection efficiency an order of magnitude higher than conventional gene transfections. Hyaluronic acids were tethered onto the QD–amPEI for cell-specific targeted labeling which showed the specific-to-nonspecific signal ratio over 100. The inside hydrophobic compartment was further applied for cohosting oxygen sensing phosphorescence Ru dyes along with QDs. The QD-Ru-amPEI oxygen probe showed accurate and reversible oxygen sensing capability by the ratiometric photoluminescence signals, which was successfully applied to cellular and spheroid models

Media 2: Gradient index lens based combined two-photon microscopy and optical coherence tomography

Originally published in Optics Express on 02 June 2014 (oe-22-11-12962

Media 5: Gradient index lens based combined two-photon microscopy and optical coherence tomography

Originally published in Optics Express on 02 June 2014 (oe-22-11-12962

Visualization 5: In vivo imaging of activated microglia in a mouse model of focal cerebral ischemia by two-photon microscopy

In vivo two-photon microscopy for activated microglia in ICW-bearing MCAO mice after vehicle treatment Originally published in Biomedical Optics Express on 01 September 2015 (boe-6-9-3303

Visualization 4: In vivo imaging of activated microglia in a mouse model of focal cerebral ischemia by two-photon microscopy

In vivo two-photon imaging of activated microglia in ICW in the contralateral side of MCAO Originally published in Biomedical Optics Express on 01 September 2015 (boe-6-9-3303