693 research outputs found

    Automatic Image Annotation for Semantic Image Retrieval

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    This paper addresses the challenge of automatic annotation of images for semantic image retrieval. In this research, we aim to identify visual features that are suitable for semantic annotation tasks. We propose an image classification system that combines MPEG-7 visual descriptors and support vector machines. The system is applied to annotate cityscape and landscape images. For this task, our analysis shows that the colour structure and edge histogram descriptors perform best, compared to a wide range of MPEG-7 visual descriptors. On a dataset of 7200 landscape and cityscape images representing real-life varied quality and resolution, the MPEG-7 colour structure descriptor and edge histogram descriptor achieve a classification rate of 82.8% and 84.6%, respectively. By combining these two features, we are able to achieve a classification rate of 89.7%. Our results demonstrate that combining salient features can significantly improve classification of images

    Looking for Distributed Star Formation in L1630: A Near-infrared (J, H, K) Survey

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    We have carried out a simultaneous, multi-band (J, H, K) survey over an area of 1320 arcmin^2 in the L1630 region, concentrating on the region away from the dense molecular cores and with modest visual extinctions (\leq 10 mag). Previous studies found that star formation in L1630 occurs mainly in four localized clusters, which in turn are associated with the four most massive molecular cores (Lada et al. 1991; Lada 1992). The goal of this study is to look for a distributed population of pre-main-sequence stars in the outlying areas outside the known star-forming cores. More than 60% of the pre-main-sequence stars in the active star forming regions of NGC 2024 and NGC 2023 show a near-infrared excess in the color-color diagram. In the outlying areas of L1630, excluding the known star forming regions, we found that among 510 infrared sources with the near-infrared colors ((J-H) and (H-K)) determined and photometric uncertainty at K better than 0.10 mag, the fraction of the sources with a near-infrared excess is 3%--8%; the surface density of the sources with a near-infrared excess is less than half of that found in the distributed population in L1641, and 1/20 of that in the young cluster NGC 2023. This extremely low fraction and low surface density of sources with a near-infrared excess strongly indicates that recent star formation activity has been very low in the outlying region of L1630. The sources without a near-infrared excess could be either background/foreground field stars, or associated with the cloud, but formed a long time ago (more than 2 Myrs). Our results are consistent with McKee's model of photoionization-regulated star formation.Comment: 30 pages, 10 figures To appear in ApJ Oct 1997, Vol 48

    Cold-Dependent Expression and Alternative Splicing of Arabidopsis Long Non-coding RNAs

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    Plants re-program their gene expression when responding to changing environmental conditions. Besides differential gene expression, extensive alternative splicing (AS) of pre-mRNAs and changes in expression of long non-coding RNAs (lncRNAs) are associated with stress responses. RNA-sequencing of a diel time-series of the initial response of Arabidopsis thaliana rosettes to low temperature showed massive and rapid waves of both transcriptional and AS activity in protein-coding genes. We exploited the high diversity of transcript isoforms in AtRTD2 to examine regulation and post-transcriptional regulation of lncRNA gene expression in response to cold stress. We identified 135 lncRNA genes with cold-dependent differential expression (DE) and/or differential alternative splicing (DAS) of lncRNAs including natural antisense RNAs, sORF lncRNAs, and precursors of microRNAs (miRNAs) and trans-acting small-interfering RNAs (tasiRNAs). The high resolution (HR) of the time-series allowed the dynamics of changes in transcription and AS to be determined and identified early and adaptive transcriptional and AS changes in the cold response. Some lncRNA genes were regulated only at the level of AS and using plants grown at different temperatures and a HR time-course of the first 3 h of temperature reduction, we demonstrated that the AS of some lncRNAs is highly sensitive to small temperature changes suggesting tight regulation of expression. In particular, a splicing event in TAS1a which removed an intron that contained the miR173 processing and phased siRNAs generation sites was differentially alternatively spliced in response to cold. The cold-induced reduction of the spliced form of TAS1a and of the tasiRNAs suggests that splicing may enhance production of the siRNAs. Our results identify candidate lncRNAs that may contribute to the regulation of expression that determines the physiological processes essential for acclimation and freezing tolerance

    Method and Apparatus for Fabricating Ceramic and Metal Components via Additive Manufacturing with Uniform Layered Radiation Drying

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    A freeform extrusion fabrication process for producing three - dimensional ceramic, metal and functionally gradient composite objects, including the steps of filling a plurality of paste sources with a respective plurality of aqueous paste compositions, operationally connecting respective syringes containing respective aqueous paste compositions to a mix ing chamber, moving a first aqueous paste composition from a first respective paste source into the mixing chamber, moving a second aqueous paste composition from a second respective paste source into the mixing chamber, mixing the first and second aqueous paste compositions to define a first admixture having a first admixture composition, extruding the first admixture onto a surface to define an extruded layer having a first admixture composition, surrounding the sides of the extruded layer with an oil bath, radiatively drying the extruded layer

    A Modeling Study of the Responses of Mesosphere and Lower Thermosphere Winds to Geomagnetic Storms at Middle Latitudes

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    Thermosphere Ionosphere Mesosphere Electrodynamics General Circulation Model (TIMEGCM) simulations are diagnostically analyzed to investigate the causes of mesosphere and lower thermosphere (MLT) wind changes at middle latitudes during the 17 April 2002 storm. In the early phase of the storm, middle‐latitude upper thermospheric wind changes are greater and occur earlier than MLT wind changes. The horizontal wind changes cause downward vertical wind changes, which are transmitted to the MLT region. Adiabatic heating and heat advection associated with downward vertical winds cause MLT temperature increases. The pressure gradient produced by these temperature changes and the Coriolis force then drive strong equatorward meridional wind changes at night, which expand toward lower latitudes. Momentum advection is minor. As the storm evolves, the enhanced MLT temperatures produce upward vertical winds. These upward winds then lead to a decreased temperature, which alters the MLT horizontal wind pattern and causes poleward wind disturbances at higher latitudes

    How does temperature affect splicing events? Isoform switching of splicing factors regulates splicing of <i>LATE ELONGATED HYPOCOTYL</i> (<i>LHY</i>)

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    One of the ways in which plants can respond to temperature is via alternative splicing (AS). Previous work showed that temperature changes affected the splicing of several circadian clock gene transcripts. Here we investigated the role of RNA‐binding splicing factors (SFs) in temperature‐sensitive alternative splicing (AS) of the clock gene LATE ELONGATED HYPOCOTYL (LHY). We characterised, in wild type plants, temperature‐associated isoform switching and expression patterns for SF transcripts from a high‐resolution temperature and time series RNA‐seq experiment. In addition we employed quantitative RT‐PCR of SF mutant plants to explore the role of the SFs in cooling‐associated AS of LHY. We show that the splicing and expression of several SFs responds sufficiently rapidly and sensitively to temperature changes to contribute to the splicing of the 5’UTR of LHY. Moreover the choice of splice site in LHY was altered in some SF mutants. The splicing of the 5’UTR region of LHY has characteristics of a molecular thermostat, where the ratio of transcript isoforms is sensitive to temperature changes as modest as 2°C and is scalable over a wide dynamic range of temperature. Our work provides novel insight into SF‐mediated coupling of the perception of temperature to post‐transcriptional regulation of the clock
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